Heavy-ion beam-induced sterile mutants of verbena (Verbena×hybrida) with an improved flowering habit

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概要

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• Sterile mutants of Verbena×hybrida were isolated at high frequency from nodal cultures of developed plants irradiated with heavy-ion beams. Sixty four in vitro-cultured nodes of fertile cultivars ‘Temari Sakura’ (FS), ‘Temari Coral Pink’ (FC) and ‘Temari White’ (FW) were irradiated with 1 to 10Gy of 14N-ion beam (1890MeV). Lateral shoot development of FS, FC and FW was not affected by irradiation with up to 10Gy. After open-pollination, shoots with inflorescence forming unenlarged ovaries were selected and propagated several times by cutting. Shoots were grown to flowering and the selection process for isolating stable sterile mutants was carried out by the same method. Finally, one mutant out of 104 FS lateral shoots (5Gy), one mutant out of 115 FC shoots (5Gy) and 3 mutants out of 108 FC shoots (10Gy) were successfully isolated. With the exception of sterility all these mutants showed normal morphology. Two sterile mutants SS and SC, which were isolated from 5Gy-irradiated FS and FC, respectively, were characterized by their flowering habits. These two mutants grew well, had a larger number of inflorescences, and a better longevity compared with their parental cultivars. These results show that heavy-ion beam irradiation is an excellent tool for isolating sterile mutants without alterations in others important traits at a high frequency. In addition, the characterization of SS and SC indicated that they have different sterile phenotypes: male and female gametes of SS are non-functional, in contrast, SC exhibits self-incompatibility, which results in mutants unable to produce seeds.

• 日本植物細胞分子生物学会の論文
• 2008-03-01

著者

• SAITO Hiroyuki

  Radiation Biology Team, Nishina Accelerator Research Center, RIKEN

• RYUTO Hiromichi

  Beam Technology Team, Nishina Accelerator Research Center, RIKEN

• FUKUNISHI Nobuhisa

  Beam Technology Team, Nishina Accelerator Research Center, RIKEN

• ABE Tomoko

  Radiation Biology Team, Nishina Accelerator Research Center, RIKEN

• Abe Tomoko

  Radiation Biology Team Nishina Accelerator Res. Center Riken

• Abe Tomoko

  Radiation Biology Team Nishina Accelerator Research Center Riken

• Kanaya Takeshi

  R&d Department Suntory Flowers Ltd.

• RYUTO Hiromichi

  RIKEN Nishina Center

• HIGUCHI Yohei

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Fukunishi Nobuhisa

  Beam Technology Team Nishina Accelerator Research Center Riken

• Saito Hiroyuki

  Radiation Biology Team Nishina Accelerator Research Center Riken

• Suzuki Ken-ichi

  Production Department Suntory Flowers Ltd.

• Higuchi Youhei

  Univ. Tsukuba Ibaraki Jpn

• Hayashi Yoriko

  Riken Saitama Jpn

• Kusumi Takaaki

  R&d Department Suntory Flowers Ltd.

• Higuchi Youhei

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• KANAYA Takeshi

  R&D Department, Suntory Flowers Ltd.

• HAYASHI Yoriko

  Radiation Biology Team, RIEKN Nishina Center

• FUKUNISHI Nobuhisa

  Radiation Biology Team, RIEKN Nishina Center

• RYUTO Hiromichi

  Radiation Biology Team, RIEKN Nishina Center

• MIYAZAKI Kiyoshi

  R&D Department, Suntory Flowers Ltd.

• KUSUMI Takaaki

  R&D Department, Suntory Flowers Ltd.

• Miyazaki Kiyoshi

  R&d Department Suntory Flowers Ltd.

• Hayashi Yoriko

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Kusumi T

  Institute For Fundamental Research Suntory Ltd

• Higuchi Youhei

  National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)

• Murai Koji

  Fukui Prefectural Univ. Fukui Jpn

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