Effect of heavy ion-beam irradiation on plant growth and mutation induction in Nicotiana tabacum

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概要

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• Ion-beam mutagenesis is a highly effective way to rapidly create new cultivars. To optimize conditions for heavy ion mutagenesis, we irradiated tobacco (Nicotiana tabacum L.) tissues at various developmental stages with heavy ion beams of various doses and examined the effects of irradiation by monitoring plant growth and mutation induction. The effects differed among irradiated tissues. Sensitivity to heavy ion-beam irradiation increased in the following order: dry seeds, imbibed seeds, and culture tissues. We isolated three white flower mutants. One, BWF1, was found to be a novel mutant, in which the synthesis of proanthocyanidin was up-regulated. The others may have a mutation in some regulatory genes involved in the flavonoid biosynthetic pathway. These results suggest that the developmental state of plant tissues is critical for efficient plant mutagenesis, and that the broad spectrum of mutations may be induced by heavy ion-beam irradiation at molecular level.

• 日本植物細胞分子生物学会の論文
• 2008-03-01

著者

• MISHIBA Kei-ichiro

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• 風間 裕介

  理化学研究所仁科加速器研究センター生物照射チーム

• SAITO Hiroyuki

  Radiation Biology Team, Nishina Accelerator Research Center, RIKEN

• ABE Tomoko

  Radiation Biology Team, Nishina Accelerator Research Center, RIKEN

• KAZAMA Yusuke

  Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo

• Bae Chang-hyu

  Division Of Plant Production Science Sunchon National University

• Abe Tomoko

  Radiation Biology Team Nishina Accelerator Research Center Riken

• Abe Tomoko

  Riken Saitama Jpn

• Kanaya Takeshi

  R&d Department Suntory Flowers Ltd.

• Kazama Yusuke

  RIKEN Nishina Center

• Saito Hiroyuki

  RIKEN Nishina Center

• MII Masahiro

  Graduate School of Horticulture, Chiba University

• MIYAZAWA Yutaka

  Graduate School of Life Sciences, Tohoku University

• SUZUKI Kenichi

  Suntory Flowers, Ltd.

• ICHIDA Hiroyuki

  RIKEN Nishina Center

• Takehisa Hinako

  Riken Nishina Center

• MIYOSHI Kazumitsu

  Faculty of Bioresource Sciences, Akita Pref. University

• Mishiba Kei-ichiro

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Saito Hiroyuki

  Radiation Biology Team Nishina Accelerator Research Center Riken

• Suzuki Ken-ichi

  Production Department Suntory Flowers Ltd.

• Suzuki Kenichi

  Suntory Flowers Ltd.

• Abe Tomoko

  Riken

• Mii Masahiro

  Graduate School Of Horticulture Chiba University

• Mii Masahiro

  Plant Cell Technol. Lab. Fac. Of Horticulture Chiba Univ.

• Kazama Yusuke

  Department Of Integrated Biosciences Graduate School Of Frontier Sciences University Of Tokyo

• Kazama Yusuke

  Univ. Tokyo Chiba Jpn

• MIYAGAI Mashu

  RIKEN Nishina Center

• KANAYA Takeshi

  Suntory Flowers Ltd.

• Miyazaki Kiyoshi

  R&d Department Suntory Flowers Ltd.

• Miyoshi Kazumitsu

  Faculty Of Bioresource Sciences Akita Pref. University

• Miyazawa Yutaka

  Graduate School Of Life Sciences Tohoku University

• Murai Koji

  Fukui Prefectural Univ. Fukui Jpn

• Saito Hiroyuki

  Riken

• Mii Masahiro

  Graduate School Of Horticulture Chiba Univ.

• 風間 裕介

  理化学研究所イノベーション推進センター

• Kazama Yusuke

  RIKEN Innovation Center

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