An Effective Method for Detection and Analysis of DNA Damage Induced by Heavy-Ion Beams
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概要
- 論文の詳細を見る
We have developed an efficient system to detect and analyze DNA mutations induced by heavy-ion beams in Arabiopsis thaliana. In this system, a stable transgenic Arabidopsis line that constitutively expresses a yellow fluorescent protein (YFP) by a single-copy gene at a genomic locus was constructed and irradiated with heavy-ion beams. The YFP gene is a target of mutagenesis, and its loss of function or expression can easily be detected by the disappearance of YFP signals in planta under microscopy. With this system, a 12C6+-induced mutant with single deletion and multiple base changes was isolated.
- 社団法人 日本農芸化学会の論文
- 2007-11-23
著者
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風間 裕介
理化学研究所仁科加速器研究センター生物照射チーム
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SAITO Hiroyuki
Radiation Biology Team, Nishina Accelerator Research Center, RIKEN
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RYUTO Hiromichi
Beam Technology Team, Nishina Accelerator Research Center, RIKEN
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FUKUNISHI Nobuhisa
Beam Technology Team, Nishina Accelerator Research Center, RIKEN
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ABE Tomoko
Radiation Biology Team, Nishina Accelerator Research Center, RIKEN
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KAZAMA Yusuke
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
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Abe Tomoko
Radiation Biology Team Nishina Accelerator Research Center Riken
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Abe Tomoko
Riken Saitama Jpn
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Kazama Yusuke
RIKEN Nishina Center
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Saito Hiroyuki
RIKEN Nishina Center
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Fujiwara Makoto
RIKEN Nishina Center
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MATSUYAMA Tomoki
Cellular Biochemistry Laboratory, RIKEN
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HAYASHI Yoriko
RIKEN Nishina Center
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RYUTO Hiromichi
RIKEN Nishina Center
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FUKUNISHI Nobuhisa
RIKEN Nishina Center
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HIGUCHI Yohei
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Fukunishi Nobuhisa
Beam Technology Team Nishina Accelerator Research Center Riken
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Saito Hiroyuki
Radiation Biology Team Nishina Accelerator Research Center Riken
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Higuchi Youhei
Univ. Tsukuba Ibaraki Jpn
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Abe Tomoko
Riken
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Kazama Yusuke
Department Of Integrated Biosciences Graduate School Of Frontier Sciences University Of Tokyo
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Kazama Yusuke
Univ. Tokyo Chiba Jpn
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Hayashi Yoriko
Riken Saitama Jpn
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Higuchi Youhei
Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Hayashi Yoriko
Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Fujiwara Makoto
Riken
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Higuchi Youhei
National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)
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Matsuyama Tomoki
Cellular Biochemistry Laboratory
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Saito Hiroyuki
Riken
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風間 裕介
理化学研究所イノベーション推進センター
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Kazama Yusuke
RIKEN Innovation Center
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