An Effective Method for Detection and Analysis of DNA Damage Induced by Heavy-Ion Beams

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概要

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• We have developed an efficient system to detect and analyze DNA mutations induced by heavy-ion beams in Arabiopsis thaliana. In this system, a stable transgenic Arabidopsis line that constitutively expresses a yellow fluorescent protein (YFP) by a single-copy gene at a genomic locus was constructed and irradiated with heavy-ion beams. The YFP gene is a target of mutagenesis, and its loss of function or expression can easily be detected by the disappearance of YFP signals in planta under microscopy. With this system, a 12C6+-induced mutant with single deletion and multiple base changes was isolated.

• 社団法人 日本農芸化学会の論文
• 2007-11-23

著者

• 風間 裕介

  理化学研究所仁科加速器研究センター生物照射チーム

• SAITO Hiroyuki

  Radiation Biology Team, Nishina Accelerator Research Center, RIKEN

• RYUTO Hiromichi

  Beam Technology Team, Nishina Accelerator Research Center, RIKEN

• FUKUNISHI Nobuhisa

  Beam Technology Team, Nishina Accelerator Research Center, RIKEN

• ABE Tomoko

  Radiation Biology Team, Nishina Accelerator Research Center, RIKEN

• KAZAMA Yusuke

  Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo

• Abe Tomoko

  Radiation Biology Team Nishina Accelerator Research Center Riken

• Abe Tomoko

  Riken Saitama Jpn

• Kazama Yusuke

  RIKEN Nishina Center

• Saito Hiroyuki

  RIKEN Nishina Center

• Fujiwara Makoto

  RIKEN Nishina Center

• MATSUYAMA Tomoki

  Cellular Biochemistry Laboratory, RIKEN

• HAYASHI Yoriko

  RIKEN Nishina Center

• RYUTO Hiromichi

  RIKEN Nishina Center

• FUKUNISHI Nobuhisa

  RIKEN Nishina Center

• HIGUCHI Yohei

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Fukunishi Nobuhisa

  Beam Technology Team Nishina Accelerator Research Center Riken

• Saito Hiroyuki

  Radiation Biology Team Nishina Accelerator Research Center Riken

• Higuchi Youhei

  Univ. Tsukuba Ibaraki Jpn

• Abe Tomoko

  Riken

• Kazama Yusuke

  Department Of Integrated Biosciences Graduate School Of Frontier Sciences University Of Tokyo

• Kazama Yusuke

  Univ. Tokyo Chiba Jpn

• Hayashi Yoriko

  Riken Saitama Jpn

• Higuchi Youhei

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Hayashi Yoriko

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Fujiwara Makoto

  Riken

• Higuchi Youhei

  National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)

• Matsuyama Tomoki

  Cellular Biochemistry Laboratory

• Saito Hiroyuki

  Riken

• 風間 裕介

  理化学研究所イノベーション推進センター

• Kazama Yusuke

  RIKEN Innovation Center

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