Efficient production of novel floral traits in torenia by collective transformation with chimeric repressors of Arabidopsis transcription factors
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概要
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Molecular breeding with genetic modification enables the production of novel floral traits in floricultural plants that could not be obtained by traditional breeding. To facilitate novel flower production, we collectively introduced 2 sets of 42 and 50 chimeric repressors of Arabidopsis transcription factors into Agrobacterium and then used these to co-transform torenia (Torenia fournieri). We generated 750 transgenic torenias, and identification of the transgenes revealed that more than 80% of the transgenic torenias had a single transgene. A total of 264 plants showed phenotypic modification, and 91.2% displayed modified flower colors and/or shapes, such as altered color patterns, curled petal margins, and wavy petals. These results indicated that the collective transformation system can be applied to molecular breeding of flowers. Detailed analysis of the phenotypes revealed that PETAL LOSS could control blotch sizes and that modification of cell shape could change the texture of petals. We found that the chimeric repressors of functionally unknown transcription factors also induced novel floral traits, and therefore, the transgenic torenias provide an understanding of the functions of transcription factors that could not be revealed by previous studies in Arabidopsis.
著者
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Aida Ryutaro
National Institute Of Floricultural Science National Agriculture And Food Research Organization
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OHTSUBO Norihiro
National Institute of Crop Science
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Yamaguchi Hiroyasu
National Institute Of Floricultural Science
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SASAKI Katsutomo
National Institute of Floricultural Science
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Nakatsuka Takashi
Iwate Biotechnology Res. Center
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Shikata Masahito
National Institute of Floricultural Science, National Agriculture and Food Research Organization (NA
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Narumi Takako
Faculty of Agriculture, Kagawa University
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Oshima Yoshimi
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (
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Takiguchi Yuko
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (
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Ohme-Takagi Masaru
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (
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Mitsuda Nobutaka
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (
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Higuchi Youhei
National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)
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