Chimeric AGAMOUS repressor induces serrated petal phenotype in Torenia fournieri similar to that induced by cytokinin application

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概要

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• The homeotic protein AGAMOUS (AG) terminates the floral meristem and promotes the development of stamens and carpels in Arabidopsis. Disruption of its function or expression of the chimeric AG repressor (AGSRDX) results in redundant petals, known as a double flower phenotype. To investigate whether this morphological change in Arabidopsis is applicable to ornamental flowers to increase their horticultural value, we introduced AGSRDX into torenia (Torenia fournieri Lind.) plants. Transgenic torenia plants expressing AGSRDX showed no redundancy in petal number, although they exhibited serration in petal margins, anthocyanin accumulation and morphological change in the stigma surface, and formation of extra vascular bundles in petals and styles. Anatomical observation of petals and styles revealed that these phenotypes are highly similar to those of forchlorfenuron (CPPU)-treated torenia plants especially in the derangement of vascular bundles. Phenotypes similar to AGSRDX transgenic torenia plants were also observed when the chimeric repressors for torenia C-function genes TfFAR or TfPLE1, homologs of Antirrhinum FARINELLI and PLENA respectively, were expressed. These results suggest that the morphological changes in AGSRDX transgenic torenia plants are induced by the disruption of C-function. These novel phenotypes might be caused by the modification of cytokinin-dependent regulation in vascular bundle formation and ectopic expression of the chimeric repressors in all whorls by the cauliflower mosaic virus (CaMV) 35S promoter.

• 日本植物細胞分子生物学会の論文
• 2008-03-01

著者

• Niki Tomoya

  National Institute of Agrobiological Resources

• Aida Ryutaro

  National Institute Of Floricultural Science National Agriculture And Food Research Organization

• Aida Ryutaro

  National Institute Of Floricultural Science National Agricultural Research Organization

• Ohme Takagi

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• OHTSUBO Norihiro

  National Institute of Crop Science

• NISHIJIMA Takaaki

  National Research Institute of Vegetables, Ornamental Plants, and Tea, Ministry of Agriculture, Fore

• Ohtsubo Norihiro

  National Institute Of Floricultural Science National Agriculture And Food Research Organization

• NARUMI Takako

  National Institute of Floricultural Science

• MITSUDA Nobutaka

  Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and

• HIRATSU Keiichiro

  Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and

• Niki Tomoya

  National Institute Of Floricultural Science

• TSUKATANI Nobue

  Iwate Biotechnology Research Center

• Ohme Takagi

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• Nakatsuka Takashi

  Iwate Biotechnology Res. Center

• Ohtsubo Norihiro

  National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)

• Nishijima Takaaki

  National Research Institute Of Vegetables Ornamental Plants And Tea

• Mitsuda Nobutaka

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• Hiratsu Keiichiro

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• Mitsuda Nobutaka

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology

• Aida Ryutaro

  National Institute Of Floricultural Science

• Ohtsubo Norihiro

  National Inst. Of Floricultural Sci. (nifs) National Agriculture And Food Res. Organization (naro)

• Nishijima Takaaki

  National Institute Of Floricultural Science National Agriculture And Food Research Organization

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