Flower color modification of gentian plants by RNAi-mediated gene silencing
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概要
- 論文の詳細を見る
RNA interference (RNAi) is an efficient and powerful technique for gene silencing compared with antisense and sense suppression. Here we report adaptation of RNAi technology to modify flower colors in gentian, targeted for suppression of three anthocyanin biosynthetic genes; chalcone synthase (CHS), anthocyanidin synthase (ANS) and flavonoid 3′,5′-hydroxylase (F3′5′H). The petals of transgenic gentian plants with a suppressed CHS gene exhibited pure white to pale-blue color, while those with a suppressed ANS gene showed only pale-blue. The suppression of the F3′5′H gene decreased delphinidin derivatives and increased cyanidin derivatives, and led to magenta flower colors. Northern blot analyses confirmed that all transgenic gentian plants showing typical phenotypes had strongly suppressed transcriptions of the targeted genes, corresponding with a change in anthocyanin accumulation and composition in their petals. Some rolCpro-CHSir transgenic gentians exhibited bicolor phenotypes with reduced anthocyanin accumulation along the vascular bundles. These data demonstrated that the suppression of anthocyanin biosynthetic genes by RNAi was successfully applied to gentian plants to change flower color, and this could be useful for designing novel flower color and patterns. Transgenic gentian plants produced in this study might be utilized as elite materials in the breeding of gentian plants in the near future.
- 日本植物細胞分子生物学会の論文
- 2008-03-01
著者
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箕作 和彦
大阪府立大学院生命環境科学研究科
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KIBA Akinori
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
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YAMAMURA Saburo
Iwate Biotechnology Research Center
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Nakatsuka Takashi
Iwate Biotechnology Research Center
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Mitsukuri Kazuhiko
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Mishiba Keiichirou
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Nishihara Masahiro
Iwate Biotechnology Research Center
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Abe Yoshiko
Iwate Biotechnology Research Center
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Kiba Akinori
Iwate Biotechnology Research Center
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TSUKATANI Nobue
Iwate Biotechnology Research Center
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KAKIZAKI Yuko
Iwate Biotechnology Research Center
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MISHIBA Kei-ichiro
Iwate Biotechnology Research Center
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Nakata Masaru
Bioproduction Res. Inst. National Inst. Of Advanced Industrial Sci. And Technol. (aist)
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Nakatsuka Takashi
Iwate Biotechnology Res. Center
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KUBOTA Akiko
Iwate Biotechnology Research Center
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Nishihara Masahiro
Iwate Biotechnology Res. Center
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Nishihara Masahiro
Bioproduction Res. Inst. National Inst. Of Advanced Industrial Sci. And Technol. (aist)
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Yamamura Saburo
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Yamasaki Satoshi
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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