Induction of Defense Responses by Synthetic Glycopeptides that Have a Partial Structure of the Elicitor in the Spore Germination Fluid of Mycosphaerella Pinodes

概要

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A high molecular weight elicitor (>70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of β-D-Glc-(1→6)-α-D-Man-(1→6)-D-Man, which is Ο-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including β-D-Glc-(1→6)-α-D-Man-(1→6)-D-Man-Ο-Ser(No.1) to [β-D-Glc-(1→6)-α-D-Man-(1→6)-D-Man]_3-Ο-Ser_3-Pro_3 (No.9) on the infection by M.pinodes, superoxide generation and ATPase activity were measured. The glycopeptides [β-D-Glc-(1→6)-α-D-Man-(1→6)-D-Man]-Ο-Ser_2-Pro_2 (No.3) to No.9 induced rejection reaction of pea tissue against M.pinodes. The glycopeptides No.3 to No.9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No.3 to No.9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No.3 to No.9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.
日本植物生理学会の論文