Suppression of Pisatin Production and ATPase Activity in Pea Plasma Membranes by Orthovanadate, Verapamil and a Suppressor from Mycosphaerella pinodes
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概要
- 論文の詳細を見る
A pea pathogen, Mycosphaerella pinodes, secretes both an elicitor and a suppressor for the accumulation of pisatin, a major phytoalexin of pea, into the spore germination fluid. The effects of the elicitor and the suppressor on the ATPase activity in pea plasma membranes was examined. The ATPase was sensitive to orthovanadate and dicyclohexylcarbodiimide but insensitive to nitrate and azide; it was unaffected by the elicitor but was markedly inhibited by the suppressor (50 μg・ml^<-1>, bovine serum albumin equivalents) or verapamil (100 μM). The accumulation of pisatin induced by the elicitor was delayed for 3 to 6 h in the presence of orthovanadate or verapamil to an extent similar to that in the presence of the suppressor. The relationship between the inhibition of plasma membrane ATPase activity and the suppression of the active defense reaction that involves the production of pisatin in the pea plant is discussed.
- 日本植物生理学会の論文
著者
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Yamada T
Laboratory Of Plant Pathology & Genetic Engineering Faculty Of Agriculture Okayama University
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YOSHIOKA Hirofumi
Grad. Sch. Bioagr. Sci., Nagoya Univ.
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ICHINOSE Yuki
Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
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SHIRAISHI Tomonori
Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University
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YAMADA Tetsuji
Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University
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Oku Hachiro
Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University
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Yamada Tetsuji
Laboratory Of Genetic Engineering Faculty Of Agriculture Okayama University
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Shiraishi Tomonori
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
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Yoshioka Hirofumi
Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University Ok
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Oku Hachiro
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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Ichinose Yuki
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
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Yoshioka H
Grad. Sch. Bioagr. Sci. Nagoya Univ.
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Yoshioka Hirofumi
Laboratory Of Defense In Plant-pathogen Interactions Graduate School Of Bioagricultural Sciences Nag
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ICHINOSE Yuki
Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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SHIRAISHI Tomonori
Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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