Functional Analysis of the Promoters of Phenylalanine Ammonia-Lyase Gene in Pea : ENVIRONMENTAL AND STRESS RESPONSES : GENES STRUCTURE AND EXPRESSION
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概要
- 論文の詳細を見る
Pycnospore germination fluid of Mycosphaerella pinodes, a fungus pathogenic on pea, contains both an elicitor and a suppressor of the accumulation of pisatin, a major phytoalexin of pea. Transcription of the genes encoding key enzymes in the biosynthesis of pisatin, namely PAL (a gene encoding phenylalanine ammonia-lyase) and CHS (a gene encoding chalcone synthase), was shown to be activated upon the treatment of pea epicotyl tissues with the fungal elicitor and suppressed upon treatment with the fungal suppressor. To investigate the mechanisms underlying activation and suppression of plant defense genes by signal molecules secreted by a fungal pathogen and other stresses, such as ultraviolet (UV) light, we constructed chimeric genes composed of the 5'-flanking regions of two members of the PSPAL family (the genes encoding phenylalanine ammonia-lyase in Pisum sativum) fused to a bacterial gene for chloramphenicol acetyltransferase. Then, the cis-regulatory elements necessary for elicitor-mediated activation and sup-pressor-mediated suppression were examined in pea protoplasts. Functional analysis of 5' nested deletions of PSPALI and PSPAL2 suggested that an enhancer-like element is located in the TATA-distal region (- 2,196 to -406) in PSPAL2. A cis-acting element(s) responsible for elicitor-mediated activation was found in the TATA-proximal region ( - 340 to - 95 in PSPAL1; - 406 to - 1 58 in PSPAL2), in which the consensus sequence motifs known as box 1 , box 2 and box 4 [Yamada et al. (1992) Plant Cell Physiol. 33: 715, Lois et al. (1989) EMBO J. 8: 1641] were present in close proximity. Furthermore, both promoters were activated by UV light but were partially suppressed in response to the fungal suppressor.
- 日本植物生理学会の論文
著者
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Hashimoto T
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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Seki Hikaru
Laboratory Of Plant Pathology & Genetic Engineering Faculty Of Agriculture Okayama University
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Kunoh Hitoshi
Institute For Biological Process Research Akatsuka Garden Co. Ltd.
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Kunoh H
Mie Univ. Tsu‐city Jpn
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Yamada T
Laboratory Of Plant Pathology & Genetic Engineering Faculty Of Agriculture Okayama University
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Takahara H
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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SHIRAISHI Tomonori
Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University
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YAMADA Tetsuji
Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University
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Sriprasertsak Permpong
Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University
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Kato Hisaharu
Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University
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Hashimoto Tadaaki
Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University
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Shimizu Hisatsugu
Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University
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Permpong Srjprasertsak
岡山大農
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Kato H
Tokyo Metropolitan Univ. Tokyo Jpn
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Yamada Tetsuji
Laboratory Of Genetic Engineering Faculty Of Agriculture Okayama University
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Shiraishi Tomonori
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
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Sriprasertsak Permpong
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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Sriprasertsak Permpong
Cetral Laboratory And Green House Complex Kamphaengsaen Campus Kasetsart University
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Shimizu Hisatsugu
Laboratory Of Plant Pathology & Genetic Engineering Faculty Of Agriculture Okayama University
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SHIRAISHI Tomonori
Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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