Expression of Atropa belladonna Putrescine N-Methyltransferase Gene in Root Pericycle
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概要
- 論文の詳細を見る
The cDNAs encoding putrescine N-methyltransferase (PMT), which catalyzes the S-adenosylmethionine-dependent N-methylation of putrescine at the first committed step in the biosynthetic pathways of tropane alkaloids, were isolated from Atropa belladonna and Hyoscyamus niger. These PMTs, however, lacked the N-terminal tandem repeat arrays previously found in Nicotiana PMTs. AbPMT1 RNA was much more abundant in the root of A.belladonna than was AbPMT2 RNA. The 5'-flanking region of the AbPMT2 gene was fused to the β-glucuronidase (GUS) reporter gene and transferred to A.belladonna. Histochemical analysis showed that GUS is expressed specifically in root pericycle cells and that the 0.3-kb 5'-upstream region was sufficient for pericycle-specific expression. Treatment of A.belladonna roots with methyl jasmonate did not up-regulate the expression of GUS or endogenous AbPMT genes. The regulation of tropane alkaloid biosynthesis is discussed and compared with that of nicotine biosynthesis.
- 日本植物生理学会の論文
著者
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Hashimoto T
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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Hashimoto Takashi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Hiramoto Toshirou
Device Development Center Hitachi Ltd.
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Yamada Yasuyuki
Grad. School Of Biol. Sci. Naist
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Yamada Yasuyuki
Naist
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Yamada Yasuyuki
Graduate School Of Biological Science Nara Institute Of Science And Technoiogy
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Yamada Yasuyuki
Present Address: Graduate School Of Biological Science Nara Institute Of Science And Technology
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Hashimoto Takashi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Suzuki Ken-ichi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Yamada Y
Present Address: Graduate School Of Biological Science Nara Institute Of Science And Technology
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Hashimoto T
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Suzuki Kaoru
Plant Molecular Biology Laboratory Molecular Biology Department National Institute Of Bioscience And
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Hashimoto Takashi
Graduate School Of Biol. Sci.
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Hashimoto Takashi
Graduate School of Agricultural Science, Kobe University
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