Harpin_<psta> from Pseudomonas syringae pv. tabaci Is Defective and Deficient in Its Expression and HR-inducing Activity
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概要
- 論文の詳細を見る
To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P.s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using recombinant harpin indicated harpin_<psta> was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P.s. pathovars in hrp-inducing medium, the truncated harpin_<psta> was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant of the host-parasite specificity in P.s. pv. tabaci. (Received August 10, 2000 ; Accepted December 21, 2000)
- 日本植物病理学会の論文
- 2001-05-25
著者
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Yamada T
Laboratory Of Plant Pathology & Genetic Engineering Faculty Of Agriculture Okayama University
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TAGUCHI Fumiko
Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
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Toyoda Kazuhiro
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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Yamada Tetsuji
Laboratory Of Genetic Engineering Faculty Of Agriculture Okayama University
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Shiraishi Tomonori
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
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Andi Salamah
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
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Ichinose Yuki
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
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Takeuchi Kasumi
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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Imura Yoshiyuki
Laboratory of Plant Pathology&Genetic Engineering, College of Agriculture, Okayama University
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Toyoda K
Laboratory Of Plant Pathology And Genetic Engineering Graduate School Of Natural Science And Technol
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Taguchi F
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
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Taguchi Fumiko
Laboratory Of Plant Pathology & Genetic Engineering Faculty Of Agriculture Okayama University
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Tanaka Rui
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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KINOSHITA Sayuri
Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University
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Kinoshita Sayuri
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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ICHINOSE Yuki
Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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SHIRAISHI Tomonori
Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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