Effect of Harpin from Four Pathovars of Pseudomonas syringae on Pea Defense Responses (Short Communication)
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概要
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To investigate the role of the proteinaceous elicitor, harpin, on host and nonhost plants, we isolated the harpin-coding gene, hrpZ, from Pseudomonas syringae pvs. pisi, glycinea, tabaci and tomato. Effects of the recombinant harpin proteins on pea plants were analyzed and compared with the effects of the corresponding bacterial treatment. After inoculation of pea with pea pathogen P. syringae pv. pisi, the bacterial population increased and the accumulation of PAL-mRNA and pisatin was inhibited. The nonpathogenic pathovars, glycinea, tabaci and tomato induced both defense responses in pea. However, none of the harpins induced the hypersensitive reaction or accumulation of PAL-mRNA and pisatin in pea. Harpins from P. syringae pvs. glycinea, tomato and pisi did induce these defense responses in tobacco, however, suggesting that externally applied harpins either are not recognized or are nonfunctional in pea plants. (Received July 27, 2000 ; Accepted February 21, 2001)
- 2001-05-25
著者
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Yamada T
Laboratory Of Plant Pathology & Genetic Engineering Faculty Of Agriculture Okayama University
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TAGUCHI Fumiko
Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
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Toyoda Kazuhiro
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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Yamada Tetsuji
Laboratory Of Genetic Engineering Faculty Of Agriculture Okayama University
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Shiraishi Tomonori
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
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Ichinose Yuki
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
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Takeuchi Kasumi
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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Toyoda K
Laboratory Of Plant Pathology And Genetic Engineering Graduate School Of Natural Science And Technol
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Taguchi F
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
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Taguchi Fumiko
Laboratory Of Plant Pathology & Genetic Engineering Faculty Of Agriculture Okayama University
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Tanaka Rui
Laboratory Of Plant Pathology And Genetic Engineering College Of Agriculture Okayama University
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ICHINOSE Yuki
Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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SHIRAISHI Tomonori
Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
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