Temporal and Spatial Pattern of Expression of the Pea Phenylalanine Ammonia-Lyase Gene1 Promoter in Transgenic Tobacco
スポンサーリンク
概要
- 論文の詳細を見る
Genes encoding phenylalanine ammonia-lyase (PAL) form a small multigene family with at least three members in pea. Tissue-specific expression of the promoter of a member of PAL gene family (PSPAL1) was investigated in the transgenic tobacco transformants carrying the different modes of chimeric fusion between the PSPAL1 promoter and a bacterial β-glucuronidase (GUS) gene. In stems, at least, strict correlation was found between steady-state levels of Gus-mRNA and enzyme activity. Significantly high level of GUS activity was observed in roots, particularly in meristematic tissues and the pigmented region of petals of transgenic tobacco carrying the translational fusion type B (-1,394 to +140 of PSPAL1 connected to Gus), followed by moderately high level of GUS activity carrying the translational fusion type A (-1,394 to +117). GUS expression in tissues of mature leaves, however, was very low in these constructs. Extremely low GUS activity was observed in the transformants of transcriptional fusion type (-1,394 to +5), whilst no activity was detected carrying non-transcription fusion type (-1,394 to -27). Furthermore, the pattern of the PSPAL1 expression was characterized in response to pathogen ingress and woundings in transgenic tobacco carrying the translational fusion type B. Woundings itself triggered marked expression of PSPAL1-driven GUS expression at the wounded sites. Inoculation of nonpathogens, Phytophthora capsici. P. boehmeriae and Erisiphe graminis f. sp. hordei, both caused rapid and very clear GUS expression zone along with the development of hypersensitive cell death area where callose was accumulated, however, the inoculation of a pathogen, P. nicotiana caused slow and hazy GUS expression zone along with the lesion development. These results suggest that the expression of pea PSPAL1 promoter is regulated in a similar fashion, at least in a part, in pea and transgenic tobacco, under the plant development and various environmental cues.
- 日本植物生理学会の論文
著者
-
Kunoh Hitoshi
Institute For Biological Process Research Akatsuka Garden Co. Ltd.
-
Kunoh H
Mie Univ. Tsu‐city Jpn
-
Yamada T
Laboratory Of Plant Pathology & Genetic Engineering Faculty Of Agriculture Okayama University
-
ICHINOSE Yuki
Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
-
SHIRAISHI Tomonori
Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University
-
YAMADA Tetsuji
Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University
-
Saitoh K
Nippon Steel Corp. Kawasaki Jpn
-
Yamada Tetsuji
Laboratory Of Genetic Engineering Faculty Of Agriculture Okayama University
-
Shiraishi Tomonori
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
-
OZAKI Miho
Laboratory of Plant Pathology and GeneticEngineering, College of Agriculture, Okayama University
-
Ozaki Miho
Laboratory Of Plant Pathology&genetic Engineering College Of Agriculture Okayama University
-
Ichinose Yuki
Laboratory Of Plant Pathology And Genetic Engineering Faculty Of Agriculture Okayama University
-
Kawamata Shinji
Takasago Perfumery Inc.
-
Shimoharai Koji
Nihon Medi+physics
-
Imura Yoshiyuki
Laboratory of Plant Pathology&Genetic Engineering, College of Agriculture, Okayama University
-
Kunoh Hitoshi
College of Biological Resources, Mie University
-
Shimoharai Koji
Laboratory Of Plant Pathology And Geneticengineering College Of Agriculture Okayama University
-
ICHINOSE Yuki
Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
-
SHIRAISHI Tomonori
Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
関連論文
- Two Bioassay Methods to Evaluate Root-Accelerating Activity of Streptomyces sp. MBR52 Metabolites
- The floral inoculating protocol: a simplified Arabidopsis thaliana transformation method modified from floral dipping
- The Ceramic Bead That Is Suitable for A Carrier of Plant-Rooting Accelerator, Streptomyces sp. MBR-52
- An Endophytic Actinomycete, Streptomyces sp. MBR-52, That Accelerates Emergence and Elongation of Plant Adventitious Roots
- The floral inoculating protocol : a simplified Arabidopsis thaliana transformation method modified from floral dipping
- Catalog of Micro-Tom tomato responses to common fungal, bacterial, and viral pathogens
- A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms
- Identification of genes expressed during spore germination of Mycosphaerella pinodes
- Agrobacterium tumefaciens-mediated transformation as a tool for random mutagenesis of Colletotrichum trifolii
- Gramine Increase Associated with Rapid and Transient Systemic Resistance in Barley Seedlings Induced by Mechanical and Biological Stresses
- SYSTEMIC ACQUIRED RESISTANCE IN BARLEY SEEDLINGS
- Functional Analysis of the Promoters of Phenylalanine Ammonia-Lyase Gene in Pea : ENVIRONMENTAL AND STRESS RESPONSES : GENES STRUCTURE AND EXPRESSION
- Phenylalanine Ammonia-Lyase Genes from Pisum sativum : Structure, Organ-Specific Expression and Regulation by Fungal Elicitor and Suppressor : GENE STRUCTURE AND EXPRESSION
- High-throughput screening for plant defense activators using a β-glucuronidase-reporter gene assay in Arabidopsis thaliana
- Phylogenetic Classification of Dof- type Transcription Factors in Pea (Pisum sativum)
- Molecular Cloning of cDNA for a Novel Pea Dof Protein, PsDof1, and Its DNA-Binding Activity to the Promoter of PsDof1 Gene
- Suppressors : Determinants of Specificity Produced by Plant Pathogens : MEMBRANES AND BIOENERGETICS : CELL-CELL INTERACTION
- Inhibition of ATPase Activity in Pea Plasma Membranes by Fungal Suppressors from Mycosphaerella pinodes and Their Peptide Moieties
- Two Suppressors, Supprescins A and B, Secreted by a Pea Pathogen, Mycosphaerella pinodes : CELL-CELL INTERACTION : NATURAL PRODUCTS
- Functional Analysis of Retrotransposons in Pea
- Changes in in vivo DNA-protein Interactions in Pea Phenylalanine Ammonia-lyase and Chalcone Synthase Gene Promoter Induced by Fungal Signal Molecules
- In vivo FOOTPRINTING ANALYSIS OF cis-REGULATORY ELEMENTS INVOLVED IN THE ELICITOR-MEDIATED ACTIVATION AND SUPPRESSOR-MEDIATED DEACTIVATION OF PEA PAL AND CHS
- ISOLATION AND CHARACTERIZATION OF ELICITOR-RESPONSIVE GENES IN PEA
- Combined Effects of Multiple cis-Acting Elements in Elicitor-Mediated Activation of PSCHS1 Gene
- ANALYSIS OF cis-REGULATORY ELEMENTS INVOLVED IN THE REGULATION OF CHALCONE SYNTHASE GENE IN PEA BY ELICITOR.
- TRANSCRIPTIONAL REGULATION OF THE GENEENCODING CHALCONE SYNTHASE IN PEA BY FUNGAL ELICITOR
- Fistupyrone, a Novel Inhibitor of the Infection of Chinese Cabbage by Alternaria brassicicola, from Streptomyces sp. TP-A0569
- Multiplication of isolate R-5 of Streptomyces galbus on rhododendron leaves and its production of cell wall-degrading enzymes
- Disease Resistance of Tissue-cultured Seedlings of Rhododendron after Treatment with Streptomyces sp. R-5
- Association of Induced Disease Resistance of Rhododendron Seedlings with Inoculation of Streptomyces sp. R-5 and Treatment with Actinomycin D and Amphotericin B to the Tissue-culture Medium
- Studies on Endophytic Actinomycetes (I) : Streptomyces sp. Isolated from Rhododendron and Its Antifungal Activity
- REGULATION OF THE GENE ENCODING PHENYLALANINE AMMONIA-LYASE OF PEA IN TRANSGENIC TOBACCO
- Inhibition of ATPase Activity in Pea Plasma Membranes In Situ by a Suppressor from a Pea Pathogen, Mycosphaerella pinodes
- REGULATION OF PAL GENES IN PEA
- Isolation and Characterization of Double-Stranded RNAs from the Pea Pathogen, Mycosphaerella pinodes
- Induction of Defense Responses by Synthetic Glycopeptides that Have a Partial Structure of the Elicitor in the Spore Germination Fluid of Mycosphaerella Pinodes
- EFFECTS OF SYNTHETIC GLYCOPEPTIDE-ELICITORS ON DEFENSE RESPONSES OF PEA PLANTS
- A binding protein for fungal signal molecules in the cell wall of Pisum sativum
- dad-1, A Putative Programmed Cell Death Suppressor Gene in Rice
- INDEPENDENT PATHWAYS OF DEFENSE RESPONSES IN ELICITIN-TREATED TOBACCO BY-2 CELLS
- Characterization of a Family of Tandemly Repeated Sequences from Loderus genucinctus insulicola(Hymenoptera:Tenthredinidae)
- Post-Translational Modification of Flagellin Determines the Specificity of HR Induction
- The injurious effect of pisatin on the plasma membrane of pea
- Temporal and Spatial Pattern of Expression of the Pea Phenylalanine Ammonia-Lyase Gene1 Promoter in Transgenic Tobacco
- Orthovanadate Suppresses Accumulation of Phenylalanine Ammonia-Lyase mRNA and Chalcone Synthase mRNA in Pea Epicotyls Induced by Elicitor from Mycosphaerella pinodes
- Biological roles of monoterpene volatiles derived from rough lemon (Citrus jambhiri Lush) in citrus defense
- Pea extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen
- In Vitro Translation of α-Amylase mRNA from Dry Wheat Embryos
- A Family of Tandemly Repetitive DNA Sequences from the Fern Sawfly, Strongylogaster osmundae (Hymenoptera: Tenthredinidae)
- A Family of Highly Repeated DNA Sequences of the Fern Sawfly Hemitaxonus japonicus Complex(Hymenoptera:Tenthredinidae)
- Identification of endophytic Streptomyces sp. R-5 and analysis of its antimicrobial metabolites
- Enhanced Defense Responses in Arabidopsis Induced by the Cell Wall Protein Fractions from Pythium oligandrum Require SGT1, RAR1, NPR1 and JAR1
- Effect of Harpin from Four Pathovars of Pseudomonas syringae on Pea Defense Responses (Short Communication)
- Plant Lectins Induce the Production of a Phytoalexin in Pisum sativum : CELL-CELL INTERACTION : NATURAL PRODUCTS
- Rapid Changes in Polyphosphoinositide Metabolism in Pea in Response to Fungal Signals
- Localization and responsiveness of a cowpea apyrase VsNTPase1 to phytopathogenic microorganisms
- RECOGNITION OF FUNGAL SUPPRESSOR BY PEA PLANTS -SIGNIFICANCE OF CELL WALL-
- An Endogenous Suppressor of the Defense Response in Pisum sativum : CELL-CELL INTERACTION : NATURAL PRODUCTS
- Regulation of Polyphosphoinositide Metabolism in Pea Plasma Membranes by Elicitor and Suppressor from a Pea Pathogen, Mycosphaerella pinodes
- Suppression of Pisatin Production and ATPase Activity in Pea Plasma Membranes by Orthovanadate, Verapamil and a Suppressor from Mycosphaerella pinodes
- Agrobacterium tumefaciens-mediated transformation for random insertional mutagenesis in Colletotrichum lagenarium
- Expression pattern of melanin biosynthesis enzymes during infectious morphogenesis of Colletotrichum lagenarium
- Expression of the 12-Oxophytodienoic Acid 10,11-Reductase Gene in the Compatible Interaction between Pea and Fungal Pathogen
- Rapid pregermination and germination responses of Erysiphe pisi conidia to contact and light
- Release of the extracellular matrix from conidia of Blumeria graminis in relation to germination
- Endophytic Actinomycetes and Their Interactions with Host Plants
- Disease resistance induced by nonantagonistic endophytic Streptomyces spp. on tissue-cultured seedlings of rhododendron
- Drought Tolerance of Tissue-cultured Seedlings of Mountain Laurel (Kalmia latifolia L.) Induced by an Endophytic Actinomycete : II. Acceleration of Callose Accumulation and Lignification
- Visualization of Infection of an Endophytic Actinomycete Streptomyces galbus in Leaves of Tissue-cultured Rhododendron
- Induction of Disease Resistance in Tissue-Cultured Seedlings of Mountain Laurel after Treatment with Streptomyces padanus AOK-30
- Drought Tolerance of Tissue-cultured Seedlings of Mountain Laurel (Kalmia latifolia L.) Induced by an Endophytic Actinomycete : I. Enhancement of Osmotic Pressure in Leaf Cells
- An Endophytic Actinomycete,Sterptomyces sp.AOK-30,Isolated from Mountain Laurel and Its Antifungal Activity
- Endophytic Actinomycetes : Attractive Biocontrol Agents(Presidential Address)
- RECOGNITION OF FUNGAL SUPPRESSOR BY PEA PLANTS II
- Association between Ion Fluxes and Defense Responses in Pea and Cowpea Tissues
- RELATIONSHIP BETWEEN DEFENSE RESPONSESAND ION FLUX
- Harpin_ from Pseudomonas syringae pv. tabaci Is Defective and Deficient in Its Expression and HR-inducing Activity
- Importance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression Induced by Nonpathogenic Attack
- Cytochalasin A Inhibits the Binding of Phenylalanine Ammonia-Lyase mRNA to Ribosomes during Induction of Phytoalexin in Pea Seedlings
- STRUCTURAL AND FUNCTIONAL ANALYSES OF HARPINS FROM SEVERAL PATHOVARS OF PSEUDOMONAS SYRINGAE
- PLANT CELL WALL RECOGNIZES AND RESPONDS TO SIGNALS FROM PHYTOPATHOGENS
- Interaction between Cell Wall and Plasma Membrane via RGD Motif is Implicated in Plant Defense Responses
- SPECIFIC REGULATION OF CELL WALL-BOUND ATPASE BY THE SUPPRESSOR FROM MYCOSPHAERELLA PINODES(3) -IDENTIFICATION OF ELICITOR- AND SUPPRESSOR-BINDING PROTEINS IN CELL WALL-BOUND ATPASE FRACTION FROM PEA PLANT-
- PURIFICATION AND ANALYSIS OF ELICITOR BINDING-PROTEIN IN CELL WALL FRACTION FROM PEA
- Functional Analysis of the Putative cis-Elements Involved in Activation by Elicitor or UV and Deactivation by Suppressor in the Promoter of a Pea Gene for Phenylalanine Ammonia-lyase (PSPAL1)
- MOLECULAR EVOLUTION OF DEFENSE RELATED CHALCONE SYNTHASE GENES IN PEA
- Genomic structure of the NtPDR1 gene, harboring the two miniature inverted-repeat transposable elements, NtToya1 and NtStowaway101
- PURIFICATION AND CHARACTERIZATION OF A BINDING PROTEIN FOR CONCANAVALIN A THAT ELICITS PHYTOALEXIN PRODUCTION IN PEA(PISUM SATIVUM L. )
- THE SIGNIFICANCE OF ACTIVE OXYGEN SPEACIES IN ELICITATION OF DEFENSE RESPONSES IN PEA PLANTS
- PLANT CELL WALL - THE PRIMARY APPARATUS RECOGNIZING AND RESPONDING TO FUNGAL SIGNALS
- Specific Response of Partially Purified Cell Wall-Bound ATPases to Fungal Suppressor
- SUPEROXIDE GENERATION IN CELL WALL DURING A PLANT-PATHOGEN INTERACTION
- Specific Inhibition of Cell Wall-Bound ATPases by Fungal Suppressor from Mycosphaerella pinodes : ENVIRONMENTAL AND STRESS RESPONSES : CELL-CELL INTERACTION
- SPECIFIC INHIBITION OF CELL WALL-BOUNDATPASE BY THE SUPPRESSOR FROM PEA PATHOGEN NYCOSHAERELLA PINODES(II)
- ASSOCIATION BETWEEN DEFENSE RESPONSES AND ION-FLUX(II)
- REGULATION OF THE GENE ENCODING PHENYLALANINE AMMONIA-LYASE IN PEA BY FUNGUL ELICITOR AND SUPPRESSOR
- PLANT LECTINS INDUCE PHYTOALEXINPRODUCTION IN PISUM SATIVUM.
- Characterization of a family of tandemly repetitive DNA sequences from the fern sawfly, Strongylogaster osmundae (Hymenoptera: Tenthredinidae).
- Repetitive DNA sequence families in Hemitaxonus minomensis and H. athyrii (Hymenoptera; Tenthredinidae).
- Molecular characterization of a family of tandemly repetitive DNA sequences (pYS family) in the genus Hemitaxonus (Hymenoptera: Tenthredinidae).