Improved translation efficiency in chrysanthemum and torenia with a translational enhancer derived from the tobacco alcohol dehydrogenase gene
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概要
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We investigated the efficiency of the 5′-untranslated region (UTR) of the tobacco alcohol dehydrogenase gene (NtADH-5′UTR) as a translational enhancer in chrysanthemum (Chrysanthemum morifolium) and torenia (Torenia fournieri). Three constructs were introduced: control, “ADH−,” cauliflower mosaic virus 35S RNA (CaMV 35S) promoter::β-glucuronidase (GUS); NtADH-5′UTR with a spacer, “ADH+S,” CaMV 35S promoter::NtADH-5′UTR::25-bp spacer::GUS; and NtADH-5′UTR with no spacer, “ADH+,” CaMV 35S promoter::NtADH-5′UTR::GUS. The highest GUS activity in ADH+S and ADH+ for chrysanthemum was about 45 and 190 times, respectively, and for torenia was 12 and 22 times, respectively, than that for the ADH− plants. NtADH-5′UTR enhanced translational efficiency in both species. With the lowest translational efficiency set to 1, the relative translational efficiencies were 1 to 15 (ADH−), 47 to 568 (ADH+S), and 267 to 1360 (ADH+) for chrysanthemum, and 1 to 3 (ADH−), 47 to 114 (ADH+S), and 85 to 226 (ADH+) for torenia. NtADH-5′UTR would facilitate practical breeding and fundamental genetic research in chrysanthemum and torenia.
- 日本植物細胞分子生物学会の論文
- 2008-03-01
著者
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Kato Ko
Nara Institute Of Science And Technology
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Aida Ryutaro
National Institute Of Floricultural Science National Agriculture And Food Research Organization
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Aida Ryutaro
National Institute Of Floricultural Science National Agricultural Research Organization
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Shinmyo Atsuhiko
Nara Inst. Sci. And Technol. Nara Jpn
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Shinmyo Atsuhiko
Nara Institute Of Science And Technology
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OHTSUBO Norihiro
National Institute of Crop Science
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Shibata Michio
National Institute Of Floricultural Science:graduate School Of Life And Environmental Sciences Unive
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Shibata Michio
National Institute Of Floricultural Science National Agricultural Research Organization
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HIGUCHI Yohei
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Yamaguchi Hiroyasu
National Institute Of Floricultural Science
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Ohtsubo Norihiro
National Institute Of Floricultural Science National Agriculture And Food Research Organization
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NARUMI Takako
National Institute of Floricultural Science
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Higuchi Youhei
Univ. Tsukuba Ibaraki Jpn
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TSUKATANI Nobue
Iwate Biotechnology Research Center
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Nakatsuka Takashi
Iwate Biotechnology Res. Center
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Yamaguchi Hiroyasu
National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)
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Hayashi Yoriko
Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Ohtsubo Norihiro
National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)
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Higuchi Youhei
National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)
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Shibata Michio
National Institute Of Floricultural Science National Agriculture And Food Research Organization
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Kato Ko
Nara Inst. Sci. And Technol. Nara Jpn
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Aida Ryutaro
National Institute Of Floricultural Science
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Ohtsubo Norihiro
National Inst. Of Floricultural Sci. (nifs) National Agriculture And Food Res. Organization (naro)
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Shibata Michio
National Institute of Floricultural Science
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