Production of(S)-4-Chloro-3-Hydroxybutyrate by Microbial Resolution Using Hydrolase from Rhizobium sp. DS-S-51(BIOCHEMICAL ENGINEERING)
スポンサーリンク
概要
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(S)-4-Chloro-3-hydroxybutyrate(CHB) is essential for the synthesis of biologically and pharmacologically important compounds. Rhizobium sp. DS-S-51 isolated from soil samples showed hydrolytic activity toward (R)-CHB in the racemate to (R)-3-hydroxy-γ-butyrolactone(HL) under a simple composition of the reaction. Residual (S)-CHB was obtained with high optical purity. The gene encoding the enzyme concerned, designated CHB hydrolase, was isolated from DS-S-51, and the gene was highly expressed in Escherichia coli JM109. When the resolution of racemic methyl CHB(CHBM) as a substrate was performed using this recombinant cell, JM109(pKK-R1), the hydrolytic activity was found to be 40-fold greater than that of DS-S-51, and the maximum concentration of the substrate added increased 2-fold. Moreover, (R)-HL was also obtained with- out decreasing the optical purity compared with that when (R)-CHBM was used as a substrate.
- 2008-04-25
著者
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Kato Ko
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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SHINMYO ATSUHIKO
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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NAKAGAWA Atsushi
Research Laboratory, New Japan Radio Co., Ltd.
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Kato Ko
Graduate School Of Biological Science Nara Institute Of Science And Technology
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Shinmyo Atsuhiko
Nara Inst. Sci. And Technol. Nara Jpn
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Shinmyo Atsuhiko
Graduate School Of Biological Science Nara Institute Of Science And Technology
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SUZUKI Toshio
Research Institute for Fundamental Physics Kyoto University
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Suzuki Takahiro
Research Laboratories of DAISO Co., Ltd.
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Suzuki Toshio
Research Laboratories Of Daiso Co. Ltd.
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Suzuki Toshio
Research Insitute For Fundamental Physics Kyoto University
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Nakagawa Atsushi
Research Laboratories Of Daiso Co. Ltd.
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Nakagawa Atsushi
Research Laboratories Aquaculture Center Kyowa Hakko Kogyo Co. Ltd
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Nakagawa Atsushi
Research Laboratories Aquaculture Center Kyowa Hakko Kogyo
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Suzuki Toshio
Research And Development Department Daiso Co. Ltd.
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