Expression of a Foreign Gene in Chlamydomonas reinhardtii Chloroplast
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概要
- 論文の詳細を見る
Chimeric genes for expression of a foreign gene in the Chlamydomonas reinhardtii chloroplast were constructed. These chimeric genes are composed of the promoter from chloroplast genes, rbcL, psbA, and atpA, 5'- and 3'-untranslated regions, and the Escherichia coli β-glucuronidase(GUS)structural gene(uidA)as a foreign gene. Three types of chloroplast transformants(RG, PG, and AG), which contained the rbcL-uidA, psbA-uidA, and atpA-uidA chimeric genes integrated in the chloroplast genome, were generated by particle bombardment. The AG transformant grown under photoautotrophic conditions showed the highest GUS activity(130nmol/min/mg protein)so far reported in C.reinhardtii, and the accumulated GUS protein accounted for 0.08% of the total soluble proteins. GUS activity in RG was 12% of that in AG, and no activity was detected in PG. We also measured the GUS activity from transformants grown under heterotrophic conditions, but the culture conditions made little difference in activity levels. The difference in the amount of accumulated GUS protein in the transformants was paralleled by the difference in the level of transcripts, and the pattern of gene expression was not the same as that of the endogenous genes in the chloroplast.
- 社団法人日本生物工学会の論文
- 1999-03-25
著者
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Kato Ko
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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SHINMYO ATSUHIKO
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Kato K
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Kato Ko
Graduate School Of Biological Science Nara Institute Of Science And Technology
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Shinmyo Atsuhiko
Graduate School Of Biological Science Nara Institute Of Science And Technology
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Yoshida Kazuya
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Takaoka Yasuko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Takaoka Yasuko
Fine Chemicals Research Laboratories Kaneka Corporation
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Kawamura Kazue
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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関根 政実
奈良先端大・バイオ
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Sekine Masami
Graduate school of Biological sciences, Nara Institute of science and Technology
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ISHIKURA KIYOHIDE
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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関根 政実
Department Of Bioproduction Science Faculty Of Bioresources And Environmental Sciences Ishikawa Pref
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Sekine M
Waseda Univ. Tokyo Jpn
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Sekine Masami
Grad. Sch. Biol. Sci. Nara Inst. Sci. Tech.
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Ichikawa Katsuhiko
Nara Inst. Of Sci. And Technol. Nara Jpn
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