Transgenic Lettuce Producing a Candidate Protein for Vaccine against Edema Disease
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概要
- 論文の詳細を見る
Pig edema disease is a bacterial disease caused by Shiga toxin 2e-producing Escherichia coli belonging mainly to serotypes O138, O139, and O141. The B subunit of Shiga toxin 2e (Stx2eB) is a candidate protein for use in a vaccine against edema disease. We produced this protein in transgenic lettuce (Lactuca sativa), an edible plant that can be cultivated in a factory setting. In a transient expression system, we found that NtADH 5′-untranslated region (5′-UTR) functions as a translational enhancer in lettuce cells, and that Stx2eB accumulates most efficiently in the endoplasmic reticulum (ER) of lettuce cells. Stx2eB was produced in stable transgenic lettuce plants expressing a modified Stx2eB gene fused with the NtADH 5′-UTR and sequence encoding ER localization signals.
- 社団法人 日本農芸化学会の論文
- 2009-07-23
著者
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Kato K
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Kato Ko
Graduate School Of Biological Science Nara Institute Of Science And Technology
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ASAO Hiroshi
Nara Prefectural Agricultural Experiment Station
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Tamoi Masahiro
Department Of Advanced Bioscience Faculty Of Agriculture Kinki University
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MATSUI Takeshi
Advanced Technology Research Laboratories, Idemitsu Kosan Co., Ltd.
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SAWADA Kazutoshi
Advanced Technology Research Laboratories, Idemitsu Kosan Co., Ltd.
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Ki Misa
Advanced Technology Research Laboratories Idemitsu Kosan Co. Ltd.
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Kato Ko
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol. (naist)
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Maruta Takanori
Department Of Food And Nutrition Faculty Of Agriculture Kinki University
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Sawada Kazutoshi
Advanced Technology Research Laboratories Idemitsu Kosan Co. Ltd.
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Matsui Takeshi
Advanced Technology Research Laboratories Idemitsu Kosan Co. Ltd.
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Asao Hiroshi
Production Technic Division Nara Prefectural Agricultural Experiment Station
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