5'Untranslated Region of the HSP 18.2 Gene Contributes to Efficient Translation in Plant Cells(PLANT BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Maximizing the amount of protein translated per unit mRNA is an important goal in establishing expression systems. The 5' untranslated region (5'-UTR) of mRNA is known to play an important role in determining the rate of translation. The full length 5'-UTR from the Arabidopsis thaliana heat shock protein (HSP) gene, HSP18.2 gene, was inserted into the cloning site between the cauliflower mosaic virus 35S RNA promoter and β-glucuronidase (GUS) gene in the pBI221 plasmid. When this construct was transfected into Nicotiana tabacum (tobacco) BY-2 protoplasts, the level of protein was about 10-fold higher than that of unmodified pBI221. The accumulation of each transcript was the same level. We also demonstrated that the 5'-UTR of the HSP18.2 gene enhances the rate of translation in stable transgenic BY-2 clones and Arabidopsis T87 protoplasts. The 5'-UTRs of the other Arabidopsis HSP genes-HSP17.4, HSP81-1, HSP81-2, and HSP81-3-also conferred efficient translation. These 5'-UTRs of HSP genes may be of use in increasing the expression of foreign proteins. In combination with a strong promoter, it can be used in the development of efficient protein production systems.
- 社団法人日本生物工学会の論文
- 2003-01-25
著者
-
Kato Ko
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
SHINMYO ATSUHIKO
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
SATOH JUNKO
Research Institute for Hepatology, Toranomon Branch Hospital
-
Kato K
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
Kato Ko
Graduate School Of Biological Science Nara Institute Of Science And Technology
-
Satoh Junko
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
Shinmyo Atsuhiko
Graduate School Of Biological Science Nara Institute Of Science And Technology
-
Yoshida Kazuya
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
Sato Junko
Research Institute For Hepatology Toranomon Branch Hospital
-
Kawamura Kazue
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
Shirai M
Ibaraki Univ. Ibaraki Jpn
-
Sekine Masami
Graduate school of Biological sciences, Nara Institute of science and Technology
-
Dansako Tomoko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
Satoh Junko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
関根 政実
Department Of Bioproduction Science Faculty Of Bioresources And Environmental Sciences Ishikawa Pref
-
Sekine M
Waseda Univ. Tokyo Jpn
-
新名 惇彦
奈良先端大
-
Sekine Masami
Grad. Sch. Biol. Sci. Nara Inst. Sci. Tech.
関連論文
- Transgenic Lettuce Producing a Candidate Protein for Vaccine against Edema Disease
- Floricultural Salvia plants have a high ability to eliminate bisphenol A(ENVIRONMENTAL BIOTECHNOLOGY)
- Improving salt tolerance in plant cells
- Introduction of the Archaebacterial Geranylgeranyl Pyrophosphate Synthase Gene into Chlamydomonas reinhardtii Chloroplast
- Efficacy of lamivudine treatment in Japanese patients with hepatitis B virus-related cirrhosis
- Efficacy of 6-month interferon therapy in chronic hepatitis B virus infection in Japan
- Infection with hepatitis B virus genotype A in Tokyo, Japan during 1976 through 2001
- Peripheral CD8+/CD25+ lymphocytes may be implicated in hepatocellular injuries in patients with acute-onset autoimmune hepatitis
- Hepatocellular carcinoma in noncirrhotic young adult patients with chronic hepatitis B viral infection
- Cadmium-tolerance of transgenic Ipomoea aquatica expressing serine acetyltransferase and cysteine synthase
- Simultaneous expression of serine acetyltransferase and cysteine synthase results in enhanced sulfate uptake and increased biomass in Ipomaea aquatica
- Efficient Plant Regeneration of Ipomoea aquatica by Direct Shoot Formation from Cotyledon Segments
- Floricultural Salvia plants have a high ability to eliminate bisphenol A
- Effect of the sequence context of the AUG initiation codon on the rate of translation in dicotyledonous and monocotyledonous plant cells(PLANT BIOTECHNOLOGY)
- The 5'-Untranslated Region of the Oryza sativa Alcohol Dehydrogenase Gene Functions as a Translational Enhancer in Monocotyledonous Plant Cells(PLANT BIOTECHNOLOGY)
- Preferential Translation Mediated by Hsp81-3 5'-UTR during Heat Shock Involves Ribosome Entry at the 5'-end Rather than an Internal Site in Arabidopsis Suspension Cells(PLANT BIOTECHNOLOGY)
- Low rate of YMDD motif mutations in polymerase gene of hepatitis B virus in chronically infected patients not treated with lamivudine
- Enzymatic Synthesis of α-Arbutin by α-Anomer-Selective Glucosylation of Hydroquinone Using Lyophilized Cells of Xanthomonas campestris WU-9701
- α-Anomer-Selective Glucosylation of (+)-Catechin by the Crude Enzyme, Showing Glucosyl Transfer Activity, of Xanthomonas campestris WU-9701
- α-Anomer-Selective Glucosylation of Menthol with High Yield through a Crystal Accumulation Reaction Using Lyophilized Cells of Xanthomonas campestris WU-9701
- Selective α-Glucosylation of Eugenol by α-Glucosyl Transfer Enzyme of Xanthomonas campestris WU-9701(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Flocculating Activity of Cross-linked Poly-γ-Glutamic Acid against Bentonite and Escherichia coli Suspension Pretreated with FeCl_3 and Its Interaction with Fe^(ENVIRONMENTAL BIOTECHNOLOGY)
- Proposals for Wastewater Treatment by Applying Flocculating Activity of Cross-linked Poly-γ-Glutamic Acid(Environmental Biotechnology)
- Physicochemical Properties of Cross-linked Poly-γ-Glutamic Acid and Its Flocculating Activity against Kaolin Suspension (Environmental Biotechnology)
- Artificial Control of Transgene Expression in Chlamydomonas reinhardtii Chloroplast Using the lac Regulation System from Escherichia coli(PLANT BIOTECHNOLOGY)
- Distinct DNase I hypersensitive sites are absent from promoters of transcriptionally incompetent genes in Arabidopsis
- Mapping and Characterization of DNase I Hypersensitive Sites in Arabidopsis Chromatin
- Tobacco RETINOBLASTOMA-RELATED protein is phosphorylated by different types of cyclin-dependent kinases during the cell cycle
- Analysis of chromatin condensation states by DNase I sensitivity assay at 500-base resolution in Arabidopsis
- REGULATION OF TRANSCRIPTION OF PLASTID GENES BY LIGHT AND CIRCADIAN RHYTHM
- ANALYSIS OF LIGHT RESPONSE OF psbD/C-3 PROMOTER ACTIVITY IN MATURE WHEAT CHLOROPLASTS
- ANALYSIS OF LIGHT REGULATION OF psbD/C-3PROMOTER ACTIVITY IN MATURE WHEAT CHLOROPLASTS
- Clinical characteristics of patients infected with hepatitis B virus genotypes A, B, and C
- Molecular Cloning and Characterization of a cDNA Clone That Encodes a Cdc2 Homolog from Nicotiana tabacum
- Expression of OsHAK genes encoding potassium ion transporters in rice
- Functions of HKT transporters in sodium transport in roots and in protecting leaves from salinity stress
- High-Efficiency Secretory Production of Peroxidase Cla Using Vesicular Transport Engineering in Transgenic Tobacco(PLANT BIOTECHNOLOGY)
- TWO TYPES OF HKT HOMOLOGUES WITH DIFFERENT PROPERTIES OF Na^+ AND K^+ TRANSPORTATION IN Oryza sativa
- TRANSCRIPTIONAL REGULATION OF RICE HKT1 GENE BY K^+, Na^+
- Resistance to insects in transgenic Solanum plants expressing a peroxidase gene from horseradish
- Phylogenetic profiling of Arabidopsis genes by monitoring spatial gene expression using cDNA microarrays
- Expression of Randomly Integrated Single Complete Copy Transgenes Does not Vary in Arabidopsis thaliana
- Systematic Transient Assays of Promoter Activities for Leaf-Specific Genes Identified by Gene-Expression Profiling with cDNA Microarrays in Arabidopsis thaliana(PLANT BIOTECHNOLOGY)
- Enhanced Resistance to Blast Fungus and Bacterial Blight in Transgenic Rice Constitutively Expressing OsSBP, a Rice Homologue of Mammalian Selenium-binding Proteins
- Efficient Construction of cDNA Microarrays Utilizing Normalized cDNA Libraries of Arabidopsis thaliana
- Wound-responsive cis-Element in the 5'-Upstream Region of Cucumber Ascorbate Oxidase Gene(Biochemistry & Molecular Biology)
- 5'Untranslated Region of the HSP 18.2 Gene Contributes to Efficient Translation in Plant Cells(PLANT BIOTECHNOLOGY)
- Plant Biotechnology : Genetic Engineering to Enhance Plant Salt Tolerance(PLANT BIOTECHNOLOGY)
- 144 Tobacco LIM protein binds to PAL box and regulates the expression of wound-inducible peroxidase gene
- Isolation of Growth-Phase-Specific Promoters from Cultured Tobacco Cells
- Expression of a Foreign Gene in Chlamydomonas reinhardtii Chloroplast
- Heat-shock dependent translational regulation of Arabidopsis thaliana HSP18.2 gene
- The LIM motif of tobacco Ntlim1 protein as a DNA-binding domain
- GROWTH STIMULATION OF HYBRID ASPEN BY INTRODUCTION OF PEROXIDASE GENE
- THE EFFECTS OF CODING REGION ON ITS GENE EXPRESSION IN THE Chlamydomonas CHLOROPLAST
- A Short Period of Mannitol Stress but Not LiCl Stress Led to Global Translational Repression in Plants
- Transgene Expression Systems in Plant, a Natural Bioreactor
- Effect of Coding Regions on Chloroplast Gene Expression in Chlamydomonas reinhardtii
- Efficient Transgene Expression in Chrysanthemum, Dendranthema grandiflorum (Ramat.) Kitamura, by Using the Promoter of a Gene for Chrysanthemum Chlorophyll-a/b-binding Protein
- Improvement on Production of (R)-4-Chloro-3-Hydroxybutyrate and (S)-3-Hydroxy-γ-Butyrolactone with Recombinant Escherichia coli Cells
- Streptomyces-Derived Induction System for Gene Expression in Cultured Plant Cells(PLANT BIOTECHNOLOGY)
- Genome-Wide Analyses of Early Translational Responses to Elevated Temperature and High Salinity in Arabidopsis thaliana
- The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells
- Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit Translation is Regulated in a Small Subunit-Independent Manner in the Expanded Leaves of Tobacco
- Efficient Translation Destabilizes Transcripts in Chloroplasts of Chlamydomonas reinhardtii (PLANT BIOTECHNOLOGY)
- Production of(S)-4-Chloro-3-Hydroxybutyrate by Microbial Resolution Using Hydrolase from Rhizobium sp. DS-S-51(BIOCHEMICAL ENGINEERING)
- An E2F-Regulated Reporter Construct is Transcriptionally Activated Following the Transient Expression of Cyclin D in Plants
- The 5'-Untranslated Region of the Tobacco Alcohol Dehydrogenase Gene Functions as an Effective Translational Enhancer in Plant(PLANT BIOTECHNOLOGY)
- CHARACTERIZATION OF TOBACCO CYCLIN D IN CULTURED BY2 CELLS
- Transcription factors on abiotic stress responses in tobacco
- Determination of the in vivo distribution of nuclear matrix attachment regions using a polymerase chain reaction-based assay in Arabidopsis thaliana(GENETICS, MOLECULAR BIOLOGY AND GENE ENGINEERING)
- CHARACTERIZATION OF PLASTID-ENCODED RNA POLYMERASES WHICH SHOW DIFFERENT PROMOTER RECOGNITION PATTERNS IN WHEAT SEEDLINGS
- Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells(PLANT BIOTECHNOLOGY)
- A Short Period of Mannitol Stress but Not LiCl Stress Led to Global Translational Repression in Plants
- TRANSCRIPTIONAL MECHANISM OF THE CHLOROPLAST rbcL GENE IN Chlamydomonas reinhardtii
- Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants
- Transgenic Lettuce Producing a Candidate Protein for Vaccine against Edema Disease
- Molecular cloning and expression of a cDNA clone for Rb from Nicotiana tabacum
- Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants
- Improving salt tolerance in plant cells
- Cloning and characterization of cyclin D from Nicotiana tabacum
- Novel vector systems to accelerate functional analysis of transcription factors using chimeric repressor gene-silencing technology (CRES-T)
- Plant Biotechnology required on the earth of the 21st century
- 水生植物パックブン(Ipomoea aquatica)節組織からの植物体の再生
- CO-TRANSLATIONAL DEGRADATION OF THE TRANSCRIPTS IN CHLOROPLAST.
- Characterization of the Rice Blast Fungal Elicitor-Responsive Gene OsSBP Encoding a Homolog to the Mammalian Selenium-binding Proteins
- Plant biotechnology : a key technology in the 21st century
- An E2F-Regulated Reporter Construct is Transcriptionally Activated Following the Transient Expression of Cyclin D in Plants.
- Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells
- Activity of the C-terminal-Dependent Vacuolar Sorting Signal of Horseradish Peroxidase C1a is Enhanced by its Secondary Structure
- High level expression of transgenes by use of 5'-untranslated region of the Arabidopsis thaliana arabinogalactan-protein 21 gene in dicotyledons
- Isolation of polyphenol oxidase genes from Portulaca oleracea and evaluation of their ability to metabolize endocrine-disrupting chemicals
- Characterization of Bisphenol A Metabolites Produced by Portulaca oleracea cv. by Liquid Chromatography Coupled with Tandem Mass Spectrometry
- Effects of Air Flow on Production of Vaccine Protein against Swine Edema Disease in Transgenic Lettuce
- Effects of Air Flow on Production of Vaccine Protein against Swine Edema Disease in Transgenic Lettuce
- N-Glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana T87 cells
- Genome-Wide Analyses of Changes in Translation State Caused by Elevated Temperature in Oryza sativa
- Isolation of polyphenol oxidase genes from Portulaca oleracea and evaluation of their ability to metabolize endocrine-disrupting chemicals
- Evaluation of Recombinant Forms of the Shiga Toxin Variant Stx2eB Subunit and Non-Toxic Mutant Stx2e as Vaccine Candidates against Porcine Edema Disease
- A Computational and Experimental Approach Reveals that the 5'-Proximal Region of the 5'-UTR has a Cis-Regulatory Signature Responsible for Heat Stress-Regulated mRNA Translation in Arabidopsis