Streptomyces-Derived Induction System for Gene Expression in Cultured Plant Cells(PLANT BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
We have constructed an induction system for plant gene expression using an operator/represser gene pair of Streptomyces virginiae. In this system, the represser protein BarA dissociates from the operator sequence BARE in the presence of an inducer virginiae butanolide (VB), resulting in the induction of the transcription of the operator's downstream genes required for virginiamycin biosynthesis [Kinoshita et al, J. Bacteriol., 179, 6986-6993 (1997)]. Two vectors were constructed: one was an effector plasmid, in which BarA was driven by plant promoters, and the other was a reporter plasmid, in which the BARE sequence was incorporated into the cauliflower mosaic virus 35S promoter to express the Escherichia coli β-glucuronidase gene (GUS). An electroporationmediated gene expression assay with cultured tobacco cells showed that GUS expression from the reporter plasmid was repressed upon coexpression with the effector plasmid and that the repression was relieved by VB. The result of electroporation to insert the reporter plasmid with various numbers and positions of BAREs into tobacco cells that had been transformed with the effector plasmid showed that the GUS induction by derepression increases with the number of BAREs and with BAREs downstream rather than upstream of the TATA box. Double transformants with the effector and reporter plasmids showed 30-fold induction with VB. The induction appeared within 8h after VB addition, maximum induction being observed with 1μM VB.
- 社団法人日本生物工学会の論文
- 2006-12-25
著者
-
Yamada Yasuhiro
Department Of Biotechnology Osaka University
-
Kato Ko
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
SHINMYO ATSUHIKO
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
Nihira Takuya
International Center for Biotechnology, Osaka University
-
Kato Ko
Graduate School Of Biological Science Nara Institute Of Science And Technology
-
Kato Ko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
-
Shinmyo Atsuhiko
Graduate School Of Biological Science Nara Institute Of Science And Technology
-
Nihira Takuya
International Center For Biotechnology Osaka University
-
Shinmyo Atsuhiko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
-
Shindo Takuya
Life Science Research Laboratories, Life Science RD Center, Kaneka Corporation
-
Takahashi Tamio
Life Science Research Laboratories, Life Science RD Center, Kaneka Corporation
-
Shindo Takuya
Life Science Research Laboratories Life Science Rd Center Kaneka Corporation
-
Yamada Yasuhiro
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo
-
Yamada Yasuhiro
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Address) Depart
-
Takahashi Tamio
Life Science Research Laboratories Life Science Rd Center Kaneka Corporation
-
Nihira Takuya
International Center For Biotechnology Osaka Univ.
関連論文
- Transgenic Lettuce Producing a Candidate Protein for Vaccine against Edema Disease
- Evaluation of Migration Energy of Lithium Ions in Chalcogenides and Halides by First Principles Calculation
- Mossbauer and Infrared Studies of Reactions of Laser-Evaporated Iron Atoms with Methane
- Identification of Novel Derivative of Helvolic Acid from Metarhizium anisopliae Grown in Medium with Insect Component(BIOCHEMICAL ENGINEERING)
- Tumor formation is correlated with expression of β-catenin-accumulated crypts in azoxymethane-induced colon carcinogenesis in mice
- Carcinoid tumor in the female urethral orifice : Rare case report and a review of the literature
- Floricultural Salvia plants have a high ability to eliminate bisphenol A(ENVIRONMENTAL BIOTECHNOLOGY)
- β-Catenin (Ctnnb1) Gene Mutations in Diethylnitrosamine (DEN)-induced Liver Tumors in Male F344 Rats
- Improving salt tolerance in plant cells
- Suppression of Occurrence and Advancement of β-Catenin-accumulated Crypts, Possible Premalignant Lesions of Colon Cancer, by Selective Cyclooxygenase-2 Inhibitor, Celecoxib
- Introduction of the Archaebacterial Geranylgeranyl Pyrophosphate Synthase Gene into Chlamydomonas reinhardtii Chloroplast
- Cadmium-tolerance of transgenic Ipomoea aquatica expressing serine acetyltransferase and cysteine synthase
- Simultaneous expression of serine acetyltransferase and cysteine synthase results in enhanced sulfate uptake and increased biomass in Ipomaea aquatica
- Efficient Plant Regeneration of Ipomoea aquatica by Direct Shoot Formation from Cotyledon Segments
- SUPPRESSIVE EFFECTS OF CHLOROGENIC ACID ON N-METHYL-N-NITROSOUREA-INDUCED GLANDULAR STOMACH CARCINOGENESIS IN MALE F344 RATS
- Inhibitory Effect of NS-398, a Selective Cyclooxygenase-2 Inhibitor, on Azoxymethane-induced Aberrant Crypt Foci in Colon Carcinogenesis of F344 Rats
- Improved Methods for the Preparation of N^6-(2-Carboxyethyl)-NAD and Poly(Ethylene Glycol)-Bound NAD(H)
- Floricultural Salvia plants have a high ability to eliminate bisphenol A
- Effect of the sequence context of the AUG initiation codon on the rate of translation in dicotyledonous and monocotyledonous plant cells(PLANT BIOTECHNOLOGY)
- The 5'-Untranslated Region of the Oryza sativa Alcohol Dehydrogenase Gene Functions as a Translational Enhancer in Monocotyledonous Plant Cells(PLANT BIOTECHNOLOGY)
- Preferential Translation Mediated by Hsp81-3 5'-UTR during Heat Shock Involves Ribosome Entry at the 5'-end Rather than an Internal Site in Arabidopsis Suspension Cells(PLANT BIOTECHNOLOGY)
- Absolute Configuration of Allosamizoline, an Aminocyclitol Derivative of the Chitinase Inhibitor Allosamidin(Organic Chemistry)
- PD33 BIOSYNTHESIS OF MACROLIDE ANTIBIOTIC PATULOLIDES BY Penicillium urticae S11R59 : IDENTIFICATION OF THE ORIGIN OF CARBON ATOMS BY ^C NMR SPECTROSCOPY
- PC88 THE STRUCTURES OF INDUCING FACTORS FOR VIRGINIAMYCIN PRODUCTION IN Streptomyces virginiae
- Chemopreventive Effect of N-(2-Cyclohexyloxy-4-nitrophenyl)methane Sulfonamide (NS-398), a Selective Cyclooxygenase-2 Inhibitor, in Rat Colon Carcinogenesis Induced by Azoxymethane
- Nucleotide Sequences of Genes Encoding Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces(Biochemistry & Molecular Biology)
- A Null Mutant of the Streptomyces virginiae barA Gene Encoding a Butyrolactone Autoregulator Receptor and Its Phenotypic and Transcriptional Analysis
- Micafungin-induced immune hemolysis attacks
- Features of elderly patients admitted to a general hospital psychiatric unit
- Structure of a Novel Disulfide of 2-(N-Acetylcysteinyl)amido-2-deoxy-α-D-glucopyran-osyl-myo-inositol Produced by Streptomyces sp.
- Purification of Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces
- New Inducing Factors for Virginiamycin Production from Streptomyces antibioticus
- IM-2,a Butyrolactone Autoregulator, Induces Production of Several Nucleoside Antibiotics in Streptomyces sp. FRI-5
- 517 Oxidases Involved in the Biosynthesis of Macrolide Antibiotics Patulolides from Penicillium urticae S11R59
- 216 Biosynthesis of Patulolides : Incorporation Pattern of ^C Labeded Acetate into the Macrolide Skeleton.
- Artificial Control of Transgene Expression in Chlamydomonas reinhardtii Chloroplast Using the lac Regulation System from Escherichia coli(PLANT BIOTECHNOLOGY)
- Mapping and Characterization of DNase I Hypersensitive Sites in Arabidopsis Chromatin
- Tobacco RETINOBLASTOMA-RELATED protein is phosphorylated by different types of cyclin-dependent kinases during the cell cycle
- Reaction of Glutaraldehyde with Amino and Thiol Compounds
- Analysis and Improvement of the Synthesis of N^6-(2-Carboxyethyl)-NAD
- Preparation and Coenzymic Properties of 3-(N-Methyl)Carbamoylpyridine-Adenine Dinucleotide and 3-[N-(2-Aminoetyl)]Carbamoylpyridine-Adenine Dinucleotide
- Active Lactonizing Lipase (LipL) Efficiently Overproduced by Pseudomonas Strains as Heterologous Expression Hosts(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Modulator-Mediated Synthesis of Active Lipase of Pseudomonas sp.109 by Escherichia coli Cell-Free Coupled Transcription/Translation System
- Purification and Characterization of a Novel Extracellular Lipase Catalyzing Hydrolysis of Oleyl Benzoate from Acinetobacter nov. sp. Strain KM109
- Increased Production of Lactonizing Lipase (LipL) from Pseudomonas sp. strain 109 by Lipids and Detergents
- Requirement in trans of the Downstream limL Gene for Activation of Lactonizing Lipase form Pseudomonas sp. 109
- Giant Photo-Induced Dielectricity in SrTiO_3(Condensed Matter : Electric Structure, Electical, Magnetic and Optical Properties)
- Giant Photo-Induced Dielectricity in SrTiO_3
- Scutellariae Radix augments ulceration but attenuates proinflammatory cytokine and chemokine gene induction in the small intestine during indomethacin-induced enteropathy in mice
- Liver injury induced by lipopolysaccharide is mediated by TNFR-1 but not by TNFR-2 or Fas in mice
- Molecular Cloning and Characterization of a cDNA Clone That Encodes a Cdc2 Homolog from Nicotiana tabacum
- Expression of OsHAK genes encoding potassium ion transporters in rice
- Functions of HKT transporters in sodium transport in roots and in protecting leaves from salinity stress
- High-Efficiency Secretory Production of Peroxidase Cla Using Vesicular Transport Engineering in Transgenic Tobacco(PLANT BIOTECHNOLOGY)
- TWO TYPES OF HKT HOMOLOGUES WITH DIFFERENT PROPERTIES OF Na^+ AND K^+ TRANSPORTATION IN Oryza sativa
- TRANSCRIPTIONAL REGULATION OF RICE HKT1 GENE BY K^+, Na^+
- Resistance to insects in transgenic Solanum plants expressing a peroxidase gene from horseradish
- Phylogenetic profiling of Arabidopsis genes by monitoring spatial gene expression using cDNA microarrays
- Expression of Randomly Integrated Single Complete Copy Transgenes Does not Vary in Arabidopsis thaliana
- Systematic Transient Assays of Promoter Activities for Leaf-Specific Genes Identified by Gene-Expression Profiling with cDNA Microarrays in Arabidopsis thaliana(PLANT BIOTECHNOLOGY)
- Enhanced Resistance to Blast Fungus and Bacterial Blight in Transgenic Rice Constitutively Expressing OsSBP, a Rice Homologue of Mammalian Selenium-binding Proteins
- Efficient Construction of cDNA Microarrays Utilizing Normalized cDNA Libraries of Arabidopsis thaliana
- Wound-responsive cis-Element in the 5'-Upstream Region of Cucumber Ascorbate Oxidase Gene(Biochemistry & Molecular Biology)
- 5'Untranslated Region of the HSP 18.2 Gene Contributes to Efficient Translation in Plant Cells(PLANT BIOTECHNOLOGY)
- Isolation of Growth-Phase-Specific Promoters from Cultured Tobacco Cells
- Expression of a Foreign Gene in Chlamydomonas reinhardtii Chloroplast
- A Short Period of Mannitol Stress but Not LiCl Stress Led to Global Translational Repression in Plants
- Transgene Expression Systems in Plant, a Natural Bioreactor
- Effect of Coding Regions on Chloroplast Gene Expression in Chlamydomonas reinhardtii
- B-1-5 Recent Problems on Chrysanthemic Acids(Terpenoids)
- Image Analysis of Mycelial Morphology in Virginiamycin Production by Batch Culture of Streptomyces virginiae
- Improvement on Production of (R)-4-Chloro-3-Hydroxybutyrate and (S)-3-Hydroxy-γ-Butyrolactone with Recombinant Escherichia coli Cells
- Promotion of Antibiotic Production by High Ethanol, High NaCl Concentration, or Heat Shock in Pseudomonas fluorescens S272
- Correlation between Autoinducers and Rhomnolipids Production by Pseudomonas aeruginosa IFO 3924
- Preparation of a Demethylallosamidin Isomer Having an N,N'-Diacetylchitobiosyl Moiety and Its Potent Inhibition against Yeast Chitinase
- Synthesis of a New Allosamidin Analog, N,N'-Diacetyl-β-Chitobiosyl Allosamizoline, and Its Inhibitory Activity against Some Chitinases
- Preparation of N-Monoalkyl and O-Acyl Derivatives of Allosamidin, and Their Chitinase Inhibitory Activities
- Distribution of Virginiae Butanolides and IM-2 in the Genus Streptomyces
- Ioslation and Structure of a New Butyrolactone Autoregulator from Streptomyces sp. FRI-5
- Streptomyces-Derived Induction System for Gene Expression in Cultured Plant Cells(PLANT BIOTECHNOLOGY)
- Glutathione as an Essential Factor for Chaperon-Mediated Activation of Lactonizing Lipase(LipL)from Pseudomonas sp.109
- Anti-Allergic Phlorotannins from the Edible Brown Alga, Eisenia Arborea
- Isolation of a New Anti-Allergic Phlorotannin, Phlorofucofuroeckol-B, from an Edible Brown Alga, Eisenia arborea
- Genome-Wide Analyses of Early Translational Responses to Elevated Temperature and High Salinity in Arabidopsis thaliana
- The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells
- Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit Translation is Regulated in a Small Subunit-Independent Manner in the Expanded Leaves of Tobacco
- Efficient Translation Destabilizes Transcripts in Chloroplasts of Chlamydomonas reinhardtii (PLANT BIOTECHNOLOGY)
- Production of(S)-4-Chloro-3-Hydroxybutyrate by Microbial Resolution Using Hydrolase from Rhizobium sp. DS-S-51(BIOCHEMICAL ENGINEERING)
- Effects of Demethylallosamidin, a Potent Yeast Chitinase Inhibitor, on the Cell Division of Yeast(Biological Chemistry)
- An E2F-Regulated Reporter Construct is Transcriptionally Activated Following the Transient Expression of Cyclin D in Plants
- The 5'-Untranslated Region of the Tobacco Alcohol Dehydrogenase Gene Functions as an Effective Translational Enhancer in Plant(PLANT BIOTECHNOLOGY)
- Global DNA hypomethylation suppresses squamous carcinogenesis in the tongue and esophagus
- Development of a Transformation System in Streptomyces virginiae
- 1019 Cloning and characterization of Δ 9-fatty acid desaturase genes from Pichia, Yarrowia and Kluyveromyces
- Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells(PLANT BIOTECHNOLOGY)
- A Short Period of Mannitol Stress but Not LiCl Stress Led to Global Translational Repression in Plants
- Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants
- Improving salt tolerance in plant cells
- Effects of Air Flow on Production of Vaccine Protein against Swine Edema Disease in Transgenic Lettuce
- A Computational and Experimental Approach Reveals that the 5'-Proximal Region of the 5'-UTR has a Cis-Regulatory Signature Responsible for Heat Stress-Regulated mRNA Translation in Arabidopsis