Preparation and Coenzymic Properties of 3-(N-Methyl)Carbamoylpyridine-Adenine Dinucleotide and 3-[N-(2-Aminoetyl)]Carbamoylpyridine-Adenine Dinucleotide

スポンサーリンク

概要

• 論文の詳細を見る

• Methods for the synthesis of 3-(N-methyl)carbamoylpyridine-adenine dinucleotide (I) and 3-[N-(2-aminoethyl)]carbamoylpyridine-adenine dinucleotide (II) are described. Derivative I shows considerable relative cofactor activity (NAD=100%) for Thermus thermophilus malate dehydrogenase (59%) and for beef liver glutamate dehydrogenase (14%), but less than 3% for the other enzymes tested. Derivative II can only be reduced by beef liver glutamate dehydrogenase and Bacillus stearothermophilus malate dehydrogenase of the enzymes tested, with the relative cofactor activities of 22% and less than 0.1%, respectively. These results indicate that the nicotinamide-binding sites of most dehydrogenases are so strictly delimited that the addition of a methyl group to the carboxamide group causes great loss of the cofactor activity of NAD. The nicotinamide-binding sites of beef liver glutamate dehydrogenase and T. thermophilus malate dehydrogenase, on the other hand, seem to have open spaces where the extra alkyl groups can enter.

• 社団法人日本生物工学会の論文
• 1985-10-25

著者

• Yamada Yasuhiro

  Department Of Biotechnology Osaka University

• Yamada Yasuhiro

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• INOUE Takuya

  Department of Cardiovascular Medicine, Toho University Ohashi Medical Center

• Urabe I

  Osaka Univ. Osaka Jpn

• Urabe Itaru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Urabe Itaru

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Urabe Itaru

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Okada H

  Kansai Univ. Osaka Jpn

• Okada H

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• Okada H

  Nagaoka Univ. Technol. Niigata Jpn

• Okada H

  Nagoya City Univ. School Of Medicine Nagoya Jpn

• Okada H

  Department Of Fermentation Technology Facutly Of Enginering Osaka Univeristy

• Okada Hirosuke

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• Okada Hirosuke

  Deparment Of Fermentation Technology Osaka University

• Urabe Itaru

  Department Of Biotechnology Osaka University

• Yamada Yuzo

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• Yamada Y

  Department Of Physics Faculty Of Science Kyoto University

• Yamada Yasuhiro

  Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo

• Inoue Takuya

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

関連論文

• Evaluation of Migration Energy of Lithium Ions in Chalcogenides and Halides by First Principles Calculation
• Mossbauer and Infrared Studies of Reactions of Laser-Evaporated Iron Atoms with Methane
• 2P438 Strategy to evaluate the effect of individual E. coli protein on the protein translation machinery(48. Bioinformatics, genomics and proteomics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Tumor formation is correlated with expression of β-catenin-accumulated crypts in azoxymethane-induced colon carcinogenesis in mice
• Carcinoid tumor in the female urethral orifice : Rare case report and a review of the literature
• β-Catenin (Ctnnb1) Gene Mutations in Diethylnitrosamine (DEN)-induced Liver Tumors in Male F344 Rats
• Relationship between spontaneous echo contrast in the thoracic aorta and plasma von Willebrand factor
• PE-190 Mitral Annulus Velocity by the Tissue Doppler Method Is Associated With B-Type Natriuretic Peptide Levels(Echo/Doppler 8 (I) : PE32)(Poster Session (English))
• 2P456 The changing cell state of the transition from the predator-prey to symbiotic relationship between E. coli and D. discoideum(49. Ecology,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Suppression of Occurrence and Advancement of β-Catenin-accumulated Crypts, Possible Premalignant Lesions of Colon Cancer, by Selective Cyclooxygenase-2 Inhibitor, Celecoxib
• Escherichia coli Transformant Expressing the Glucose Dehydrogenase Gene from Bacillus megaterium as a Cofactor Regenerator in a Chiral Alcohol Production System
• SUPPRESSIVE EFFECTS OF CHLOROGENIC ACID ON N-METHYL-N-NITROSOUREA-INDUCED GLANDULAR STOMACH CARCINOGENESIS IN MALE F344 RATS
• Inhibitory Effect of NS-398, a Selective Cyclooxygenase-2 Inhibitor, on Azoxymethane-induced Aberrant Crypt Foci in Colon Carcinogenesis of F344 Rats
• Heme Content of Catalase I from Bacillus stearothermophilus
• Experimental Sketch of Landscapes in Protein Sequence Space
• 205 Conversion of catalase into peroxidase by random mutagenesis
• Revised Sequence and Activity of Bacillus stearothermophilus Catalase I (Formerly Peroxidase)
• 376. statistical Analysis of Random Mutation on the Catalase Gene
• Improved Methods for the Preparation of N^6-(2-Carboxyethyl)-NAD and Poly(Ethylene Glycol)-Bound NAD(H)
• 563 Random Mutagenesis of a Thermostable Peroxidase from ___- ___-: Distribution of Mutants.
• Characterization of Hybrid Enzymes between 6-Aminohexanoate-Dimer Hydrolase and Its Analogous Protein
• Alteration of Catalytic Function of 6-Aminohexanoate-Dimer Hydrolase by Site-Directed Mutagenesis
• Absolute Configuration of Allosamizoline, an Aminocyclitol Derivative of the Chitinase Inhibitor Allosamidin(Organic Chemistry)
• PD33 BIOSYNTHESIS OF MACROLIDE ANTIBIOTIC PATULOLIDES BY Penicillium urticae S11R59 : IDENTIFICATION OF THE ORIGIN OF CARBON ATOMS BY ^C NMR SPECTROSCOPY
• PC88 THE STRUCTURES OF INDUCING FACTORS FOR VIRGINIAMYCIN PRODUCTION IN Streptomyces virginiae
• Expression of the Xylan-Degrading Genes of Bacillus pumilus IPO in Saccharomyces cerevisiae
• Mathematical Model Allowing the Coexistence of Closely Related Competitors at the Initial Stage of Evolution
• Fate of a Mutant Emerging at the Initial Stage of Evolution
• How Small Can the Difference among Competitors Be for Coexistence to Occur
• Chemopreventive Effect of N-(2-Cyclohexyloxy-4-nitrophenyl)methane Sulfonamide (NS-398), a Selective Cyclooxygenase-2 Inhibitor, in Rat Colon Carcinogenesis Induced by Azoxymethane
• Multi-level Disruption of the Spinal Nerve Root Sleeves in Spontaneous Spinal Cerebrospinal Fluid Leakage
• Construction and Characterization of N-Terminally Truncated DNA Polymerase from Thermus thermophilus
• Nucleotide Sequences of Genes Encoding Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces(Biochemistry & Molecular Biology)
• A Null Mutant of the Streptomyces virginiae barA Gene Encoding a Butyrolactone Autoregulator Receptor and Its Phenotypic and Transcriptional Analysis
• A Novel Versatility of Catalase I as a Dioxygenase for Indole - ring - opening Dioxygenation
• Operational Stability of a Continuous Enzyme Reactor Containing Poly(ethylene glycol)-Bound NAD and Thermostable Dehydrogenases
• Effective Selection System for Experimental Evolution of Random Polypeptides Towards DNA-Binding Protein(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Synthesis of Functional Protein in Liposome
• Construction and Characterization of Phage Libraries Displaying Artificial Proteins with Random Sequences
• Kinetic Equation for Template-Dependent Polymerase Reactions and Its Application to Thermus thermophilus DNA Polymerase
• 408 The Role of Asp21 of the Xylanase from Bacillus pumilus IPO
• Micafungin-induced immune hemolysis attacks
• 2P134 Nascent chain, RNA, and ribosome complexes generated by reconstituted in vitro translation system(32. Evolutionary molecular engineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Quantification of Structural Properties of Cell-Sized Individual Liposomes by Flow Cytometry(MISCELLANEOUS)
• Functional Qβ Replicase Genetically Fusing Essential Subunits EF-Ts and EF-Tu with β-Subunit(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Kinetic Properties of Qβ Replicase, an RNA Dependent RNA Polymerase
• Random Mutagenesis of Glutamine Synthetase from Escherichia coli; Correlation between Structure, Activity, and Fitness
• Features of elderly patients admitted to a general hospital psychiatric unit
• Structure of a Novel Disulfide of 2-(N-Acetylcysteinyl)amido-2-deoxy-α-D-glucopyran-osyl-myo-inositol Produced by Streptomyces sp.
• Purification of Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces
• New Inducing Factors for Virginiamycin Production from Streptomyces antibioticus
• IM-2,a Butyrolactone Autoregulator, Induces Production of Several Nucleoside Antibiotics in Streptomyces sp. FRI-5
• 517 Oxidases Involved in the Biosynthesis of Macrolide Antibiotics Patulolides from Penicillium urticae S11R59
• 216 Biosynthesis of Patulolides : Incorporation Pattern of ^C Labeded Acetate into the Macrolide Skeleton.
• Crystal Structure of Glucose Dehydrogenase from Bacillus megaterium IWG3 at 1.7 A Resolution^1
• Characterization of Endo-Type 6-Aminohexanoate-Oligomer Hydrolase from Flavobacterium sp.
• Structure of Isozyme Genes of Glucose Dehydrogenase from Bacillus megaterium IAM1030
• Purification and Characterization of a New Glucose Dehydrogenase from Vegetative Cells of Bacillus megaterium
• Cloning, Nucleotide Sequence, and Expression in Escherichia coli of DNA Polymerase Gene (polA) from Thermus thermophilus HB8
• Reaction of Glutaraldehyde with Amino and Thiol Compounds
• Analysis and Improvement of the Synthesis of N^6-(2-Carboxyethyl)-NAD
• Preparation and Coenzymic Properties of 3-(N-Methyl)Carbamoylpyridine-Adenine Dinucleotide and 3-[N-(2-Aminoetyl)]Carbamoylpyridine-Adenine Dinucleotide
• Active Lactonizing Lipase (LipL) Efficiently Overproduced by Pseudomonas Strains as Heterologous Expression Hosts(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Modulator-Mediated Synthesis of Active Lipase of Pseudomonas sp.109 by Escherichia coli Cell-Free Coupled Transcription/Translation System
• Purification and Characterization of a Novel Extracellular Lipase Catalyzing Hydrolysis of Oleyl Benzoate from Acinetobacter nov. sp. Strain KM109
• Increased Production of Lactonizing Lipase (LipL) from Pseudomonas sp. strain 109 by Lipids and Detergents
• Requirement in trans of the Downstream limL Gene for Activation of Lactonizing Lipase form Pseudomonas sp. 109
• Giant Photo-Induced Dielectricity in SrTiO_3(Condensed Matter : Electric Structure, Electical, Magnetic and Optical Properties)
• Giant Photo-Induced Dielectricity in SrTiO_3
• Scutellariae Radix augments ulceration but attenuates proinflammatory cytokine and chemokine gene induction in the small intestine during indomethacin-induced enteropathy in mice
• Tetrazolium Salt Method Coupled with a Phenazinium Salt Gives Higher Rates for Dehydrogenase Reactions
• Liver injury induced by lipopolysaccharide is mediated by TNFR-1 but not by TNFR-2 or Fas in mice
• Deoxyribonucleic Acid Homologies in Strains of Gluconobacter Species : Taxonomic Studies on Acetic Acid Bacteria and Allied Organisms. Part VIII.
• B-1-5 Recent Problems on Chrysanthemic Acids(Terpenoids)
• Image Analysis of Mycelial Morphology in Virginiamycin Production by Batch Culture of Streptomyces virginiae
• Promotion of Antibiotic Production by High Ethanol, High NaCl Concentration, or Heat Shock in Pseudomonas fluorescens S272
• Correlation between Autoinducers and Rhomnolipids Production by Pseudomonas aeruginosa IFO 3924
• Preparation of a Demethylallosamidin Isomer Having an N,N'-Diacetylchitobiosyl Moiety and Its Potent Inhibition against Yeast Chitinase
• Synthesis of a New Allosamidin Analog, N,N'-Diacetyl-β-Chitobiosyl Allosamizoline, and Its Inhibitory Activity against Some Chitinases
• Preparation of N-Monoalkyl and O-Acyl Derivatives of Allosamidin, and Their Chitinase Inhibitory Activities
• Distribution of Virginiae Butanolides and IM-2 in the Genus Streptomyces
• Ioslation and Structure of a New Butyrolactone Autoregulator from Streptomyces sp. FRI-5
• Streptomyces-Derived Induction System for Gene Expression in Cultured Plant Cells(PLANT BIOTECHNOLOGY)
• Glutathione as an Essential Factor for Chaperon-Mediated Activation of Lactonizing Lipase(LipL)from Pseudomonas sp.109
• Anti-Allergic Phlorotannins from the Edible Brown Alga, Eisenia Arborea
• Isolation of a New Anti-Allergic Phlorotannin, Phlorofucofuroeckol-B, from an Edible Brown Alga, Eisenia arborea
• Effects of Amino Acid Substitution on the Physicochemical Properties of Artificial Proteins with Random Sequences
• Acute Subdural Hematoma Originating From the Lacerated Intracranial Internal Carotid Arteries : Case Report
• Effects of Demethylallosamidin, a Potent Yeast Chitinase Inhibitor, on the Cell Division of Yeast(Biological Chemistry)
• Global DNA hypomethylation suppresses squamous carcinogenesis in the tongue and esophagus
• Autofluorescence imaging videoendoscopy in the diagnosis of chronic atrophic fundal gastritis
• Development of a Transformation System in Streptomyces virginiae
• 1019 Cloning and characterization of Δ 9-fatty acid desaturase genes from Pichia, Yarrowia and Kluyveromyces
• DNA TYPING OF HLA IN THE PATIENTS WITH MOYAMOYA DISEASE
• Membrane-Bound Cell Surface Peptidases in Reproductive Organs
• Properties of Glucose-Dehydrogenase-Poly(Ethylene Glycol)-NAD Conjugate as an NADH-Regeneration Unit in Enzyme Reactors
• A Case of Marked Hyperlipoprotein(a)emia Associated with Nephrotic Syndrome and Advanced Atherosclerosis
• SURVEILLANCE COLONOSCOPY USING A TRANSPARENT HOOD AND IMAGE-ENHANCED ENDOSCOPY
• Giant Cell Granulomatous Hypophysitis Manifesting as an Intrasellar Mass with Unilateral Ophthalmoplegia : Case Report
• Changes in the Serum Levels of Human Chorionic Gonadotropin and the Pulsatility Index of Uterine Arteries during Conservative Management of Retained Adherent Placenta

もっと見る 閉じる

スポンサーリンク