Quantification of Structural Properties of Cell-Sized Individual Liposomes by Flow Cytometry(MISCELLANEOUS)

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概要

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• We describe a new high-throughput method of quantifying the structural properties of individual cell-sized liposomes. An internal aqueous solution of liposomes was labeled with a green fluorescent marker and the membrane with a red marker. The double-labeled liposomes were analyzed using flow cytometry, and the internal aqueous volume and lipid membrane volume of each liposome were measured. The experimental results indicate that both the internal aqueous and lipid membrane volumes positively correlate with the intensity of forward-scatter (FS) and side-scatter (SS) signals in a logarithmic scale. In addition, liposomes in 18 small areas gated by log(FS) and log(SS) were sorted by fluorescence-activated cell sorting (FACS), and observed by optical microscopy. Structural characteristics observed in the microscopy images of heterogeneous liposomes correlated with FACS data. Because this method does not employ any particular assumption about the shape and structure of liposomes, flow cytometry is a powerful tool for estimating the internal and membrane volumes of individual cell-sized liposomes with heterogeneous shapes and structures.

• 社団法人日本生物工学会の論文
• 2006-09-25

著者

• SUGAWARA Tadashi

  Department of Basic Science, Graduate School of Arts and Science, The University of Tokyo

• Yomo Tetsuya

  Department of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osa

• Yomo T

  Osaka Univ. Osaka Jpn

• Yomo Tetsuya

  Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka

• Urabe I

  Osaka Univ. Osaka Jpn

• Urabe Itaru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Urabe I

  Graduate School Of Engineering Osaka University

• Urabe Itaru

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Urabe Itaru

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Yomo Tetsuya

  Department Of Bioinformatics Engineering Graduate School Of Information Science And Technology Osaka

• Yomo Tetsuya

  Osaka University

• Urabe Itaru

  Department Of Biotechnology Osaka University

• SATO KANETOMO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Obinata Kei

  Department of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osa

• Obinata Kei

  Department Of Bioinformatics Engineering Graduate School Of Information Science And Technology Osaka

• Sato K

  Department Of Bioinformatics Engineering Graduate School Of Information Science And Technology Osaka

• Shiobara Katsumi

  Satake Chemical Equipment Mfg. Ltd.

• Urabe Itaru

  Graduate School Of Engineering Osaka University

• Yomo Tetsuya

  Erato Jst:graduate School Of Information Science And Technology Osaka University:graduate School Of

• Sugawara Tadashi

  Department Of Basic Science Graduate School Of Arts And Sciences The University Of Tokyo

• Sugawara Tadashi

  Department Of Pure And Applied Sciences Graduate School Of Arts And Sciences The University Of Tokyo

• Yomo Tetsuya

  Department Of Bioinformatics Engineering Graduate School Of Information Science And Technology Osaka

• Sugawara Tadashi

  Department Of Basic Science Graduate School Of Arts & Sciences The University Of Tokyo

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