Purification and Characterization of a New Glucose Dehydrogenase from Vegetative Cells of Bacillus megaterium

スポンサーリンク

概要

• 論文の詳細を見る

• A new type of glucose dehydrogenase was purified from vegatevi cells of Bacillus megaterium IAM1030. The characteristics of the vegetative-cell enzyme were investigated and compared with the enzyme from sporulating cells of B. megaterium IWG3. They are very similar in the following points : molecular size (M_r 120,000), subunit composition (homo tetramer), pH-activity profile with an optimum pH at around 8,pH-stability profile with a stable pH range of 6.0-7.5 (at 25℃, for 30 min), substrate specificity (specific for D-glucose and 2-deoxy-D-glucose), and the affinity for glucose (a K_m value of 11-12 mM at pH 8.0,2.5 mM NAD). They are a little different in the folloewing points : slower mobility for the vegetative-cell enzyme in polyacrylamide-gel electrophoresis at pH 8,immunological determinants (some of them are common), and higher teat resistance for the vegatative-cell enzyme at pH 6.5. They are quite different in their affinity for NAD and NADP. The K_m values for NAD are 0.1 mM for the vegatative-cell enzyme and 1.0 mM for the spore enzyme, while the values for NADP are 7.1 mM for the vegatative-cell enzyme and 0.09 mM for the spore enzyme, at pH 8.0,0.1 M D-glucose. These results suggest that B. megaterium has at least two types of glucose dehydrogenase.

• 社団法人日本生物工学会の論文
• 1989-06-25

著者

• Negoro Seiji

  Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo

• Negoro Seiji

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Negoro Seiji

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

• Urabe I

  Osaka Univ. Osaka Jpn

• Urabe Itaru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Urabe Itaru

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Urabe Itaru

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• MAKINO Yasutaka

  Department of Biology, Faculty of Science, Chiba University

• Negoro Seiji

  Department Of Biotechnology Osaka University

• Okada H

  Kansai Univ. Osaka Jpn

• Okada H

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• Okada H

  Nagaoka Univ. Technol. Niigata Jpn

• Okada H

  Nagoya City Univ. School Of Medicine Nagoya Jpn

• Okada H

  Department Of Fermentation Technology Facutly Of Enginering Osaka Univeristy

• Okada Hirosuke

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• Okada Hirosuke

  Deparment Of Fermentation Technology Osaka University

• Urabe Itaru

  Department Of Biotechnology Osaka University

• DING JIU-YUAN

  (Present address)Department of Microbial Metanolism, Institute of Microbiology, Academia Sinica, Bei

• Makino Y

  Osaka Univ. Osaka Jpn

• Ding Jiu-yuan

  (present Address)department Of Microbial Metanolism Institute Of Microbiology Academia Sinica Beijin

• Makino Yasutaka

  Department Of Biology Faculty Of Science Chiba University

関連論文

• Enzyme-linked Immunosorbent Assay for Nonylphenol Using Antibody-Bound Microfluid Filters in Vertical Fluidic Operation(METHODS)
• Immunoassay Using Microfluid Filters Constructed by Deep X-Ray Lithography
• 2P438 Strategy to evaluate the effect of individual E. coli protein on the protein translation machinery(48. Bioinformatics, genomics and proteomics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Mutational Analysis of the Metabolism of 2,6-Naphthalenedisulfonate by Pigmentiphaga sp. NDS-2
• フロック形成菌Pigmentiphaga sp. NDS-1による2,6-ナフタレンジスルホン酸の生物分解
• 2P456 The changing cell state of the transition from the predator-prey to symbiotic relationship between E. coli and D. discoideum(49. Ecology,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Escherichia coli Transformant Expressing the Glucose Dehydrogenase Gene from Bacillus megaterium as a Cofactor Regenerator in a Chiral Alcohol Production System
• Heme Content of Catalase I from Bacillus stearothermophilus
• Experimental Sketch of Landscapes in Protein Sequence Space
• 205 Conversion of catalase into peroxidase by random mutagenesis
• Revised Sequence and Activity of Bacillus stearothermophilus Catalase I (Formerly Peroxidase)
• 376. statistical Analysis of Random Mutation on the Catalase Gene
• Improved Methods for the Preparation of N^6-(2-Carboxyethyl)-NAD and Poly(Ethylene Glycol)-Bound NAD(H)
• 563 Random Mutagenesis of a Thermostable Peroxidase from ___- ___-: Distribution of Mutants.
• Characterization of Hybrid Enzymes between 6-Aminohexanoate-Dimer Hydrolase and Its Analogous Protein
• Alteration of Catalytic Function of 6-Aminohexanoate-Dimer Hydrolase by Site-Directed Mutagenesis
• Expression of the Xylan-Degrading Genes of Bacillus pumilus IPO in Saccharomyces cerevisiae
• Mathematical Model Allowing the Coexistence of Closely Related Competitors at the Initial Stage of Evolution
• Fate of a Mutant Emerging at the Initial Stage of Evolution
• Distribution of AP-2 subtypes in the adult mouse brain
• How Small Can the Difference among Competitors Be for Coexistence to Occur
• 1P042 Molecular dynamics studies on Mutational structures of a Nylon-6 Byproduct-degrading Enzyme(Protein:Structure & Function,The 48th Annual Meeting of the Biophysical Society of Japan)
• Construction and Characterization of N-Terminally Truncated DNA Polymerase from Thermus thermophilus
• A Novel Versatility of Catalase I as a Dioxygenase for Indole - ring - opening Dioxygenation
• Operational Stability of a Continuous Enzyme Reactor Containing Poly(ethylene glycol)-Bound NAD and Thermostable Dehydrogenases
• Analysis of a Promoter-Like Region in Flavobacterium Which Controls 6-Aminohexanoic Acid Linear Oligomer Hydrolase Gene Expression in Escherichia coli
• Effective Selection System for Experimental Evolution of Random Polypeptides Towards DNA-Binding Protein(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Synthesis of Functional Protein in Liposome
• Construction and Characterization of Phage Libraries Displaying Artificial Proteins with Random Sequences
• Kinetic Equation for Template-Dependent Polymerase Reactions and Its Application to Thermus thermophilus DNA Polymerase
• 408 The Role of Asp21 of the Xylanase from Bacillus pumilus IPO
• 2P134 Nascent chain, RNA, and ribosome complexes generated by reconstituted in vitro translation system(32. Evolutionary molecular engineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Quantification of Structural Properties of Cell-Sized Individual Liposomes by Flow Cytometry(MISCELLANEOUS)
• Functional Qβ Replicase Genetically Fusing Essential Subunits EF-Ts and EF-Tu with β-Subunit(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Kinetic Properties of Qβ Replicase, an RNA Dependent RNA Polymerase
• Random Mutagenesis of Glutamine Synthetase from Escherichia coli; Correlation between Structure, Activity, and Fitness
• Purification and Properties of Aminopeptidase from Flavobacterium sp.
• Genetic Organization of Nylon-Oligomer-Degrading Enzymes from Alkalophilic Bacterium, Agromyces sp. KY5R(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Purification and Characterization of Alkylcatechol 2,3-Dioxygenase from Butylphenol Degradation Pathway of Pseudomonas putida MT4(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Characterization of Alkylphenol Degradation Gene Cluster in Pseudomonas putida MT4 and Evidence of Oxidation of Alkylphenols and Alkylcatechols with Medium-Length Alkyl Chain(ENVIRONMENTAL BIOTECHNOLOGY)
• Crystal Structure of Glucose Dehydrogenase from Bacillus megaterium IWG3 at 1.7 A Resolution^1
• Characterization of Endo-Type 6-Aminohexanoate-Oligomer Hydrolase from Flavobacterium sp.
• Structure of Isozyme Genes of Glucose Dehydrogenase from Bacillus megaterium IAM1030
• Purification and Characterization of a New Glucose Dehydrogenase from Vegetative Cells of Bacillus megaterium
• Cloning, Nucleotide Sequence, and Expression in Escherichia coli of DNA Polymerase Gene (polA) from Thermus thermophilus HB8
• Reaction of Glutaraldehyde with Amino and Thiol Compounds
• Analysis and Improvement of the Synthesis of N^6-(2-Carboxyethyl)-NAD
• Preparation and Coenzymic Properties of 3-(N-Methyl)Carbamoylpyridine-Adenine Dinucleotide and 3-[N-(2-Aminoetyl)]Carbamoylpyridine-Adenine Dinucleotide
• Tetrazolium Salt Method Coupled with a Phenazinium Salt Gives Higher Rates for Dehydrogenase Reactions
• Simultaneous Degradation of 4-Nitrophenol and Picric Acid by Two Different Mechanisms of Rhodococcus sp. PN1
• Cloning and Characterization of a 4-Nitrophenol Hydroxylase Gene Cluster from Rhodococcus sp. PN1
• Properties of (Ti, Al) N films prepared by ion beam assisted deposition(Physics, Process, Instrument & Measurements)
• Purification and Characterization of Catechol 2,3-Dioxygenase from the Aniline Degradation Pathway of Acinetobacter sp. YAA and Its Mutant Enzyme, Which Resists Substrate Inhibition
• Interaction of the Escherichia coli Lipoprotein NlpI with Periplasmic Prc (Tsp) Protease
• Effects of Amino Acid Substitution on the Physicochemical Properties of Artificial Proteins with Random Sequences
• A Cost-Effective Dynamic Content Migration Method in CDNs(Network Management/Operation)
• Secretive Expression of the Aspergillus aculeatus Cellulase (FI-CMCase) by Saccharomyces cerevisiae
• Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Saccharomyces cerevisiae
• Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Escherichia coli
• L-015 A Knowledge-Based File Allocation Method for Real-Time Environments
• Load Fluctuation-Based Dynamic File Allocation with Cost-Effective Mirror Function
• Reliability-Based Mirroring of Servers in Distributed Networks
• Sensitivity Analysis in Optimal Design for Distributed File Allocation Systems
• A Simple and Rapid Extraction of High Molecular Weight Chromosomal DNA from Bacillus subtilis Protoplasts for Cosmid Cloning and Interspecific Transformation
• File Allocation Designs for Distributed Multimedia Information Networks(Special Issue on Multimedia Communications in Heterogeneous Network Environments)
• Simple Detection of Xylosidase Activity in Single Colonies, Using 1-Naphthyl-β-D-xylopyranoside
• A Simple Assay for Xylanase Using o-Nitrophenyl-β-D-xylobioside
• A Large Capacity Photonic ATM Switch Based on Wavelength Division Multiplexing Technology
• A Photonic ATM Switch Architecture for WDM Optical Networks
• Performance Study of the Multi-Cell Transfer Scheme in the Optical Backbone Network
• Influence of Ion Irradiation on Crystalline Phases of Oxide Films(Materials, Metallurgy & Weldability)
• Ti-Al Alloy Films Synthesized by Ion-Beam-Enhanced Deposition(Physics, Process, Instrument & Measurements)
• Reaction and Interfacial Characterization on Joining of Zirconia to Carbon Steel(Materials, Metallurgy & Weldability)
• Reaction of Zirconia with Carbon Steel in a Vacum Condition
• 枯草菌Marburg株の生産するα-アミラーゼの性質(ノート)〔英文〕
• Biodegradability of 3-Methyl-4-Nitrophenol by 4-Nitrophenol-Degrading Bacteria
• Purification and Gene Cloning of an Enantioselective Thioesterification Enzyme from Brevibacterium ketoglutamicum KU1073, a Deracemization Bacterium of 2-(4-Chlorophenoxy)propanoic Acid
• Immunoassay Using Microfluid Filters Constructed by Deep X-Ray Lithography
• 枯草菌Marburg株のα-アミラーゼ生産能の研究-1-高生産株の分離とそれらの酵素の比較〔英文〕
• Purification and Characterization of Catechol 2,3-Dioxygenase from the Aniline Degradation Pathway of Acinetobacter sp. YAA and Its Mutant Enzyme, Which Resists Substrate Inhibition
• Identification and characterization of another 4-nitrophenol degradation gene cluster, nps, in Rhodococcus sp. strain PN1(ENVIRONMENTAL BIOTECHNOLOGY)
• Comparison of Acyl-CoA Synthetic Activities and Enantioselectivity toward 2-Arylpropanoic Acids in Firefly Luciferases
• Properties of Glucose-Dehydrogenase-Poly(Ethylene Glycol)-NAD Conjugate as an NADH-Regeneration Unit in Enzyme Reactors
• Kinetics of α-glucoside fermentation controlled by substrater permeation (Proceedings of the U.S.Japan seminar on dynamics of microbial populations(特集))
• Kinetic Studies on Enzyme Production by Microbes : (II) Process Kinetics of α-Amylase Production by Bacillus subtilis
• Protoplast Formation from Conidia of Trichoderma reesei by Cell Wall-Lytic Enzymes of a Strain of Trichoderma viride
• Comparison of Acyl-CoA Synthetic Activities and Enantioselectivity toward 2-Arylpropanoic Acids in Firefly Luciferases
• Inherent characteristics of gene expression for buffering environmental changes without the corresponding transcriptional regulations
• Hydration of Squalene and Oleic Acid by Corynebacterium sp. S-401
• Purification and Properties of Endoxylanase Produced by Bacillus pumilus
• Physical Map of Nylon Oligomer Degradative Plasmid pOAD2 Harbored in Flavobacterium sp. KI72
• Asymmetric Synthesis of (S)-(-) Squalene-2, 3-epoxide

もっと見る 閉じる

スポンサーリンク