Directional migration of neuronal PC12 cells in a ratchet wheel shaped microchamber(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
Directional migration of neuronal cells over long distances is critical for the developing and regenerating nervous system. A scaffold, which includes radial glia, provides a route between the germinal zone and the destination, with the direction of migration being attributed mainly to chemotaxis. However, the decrease in chemokine concentration with distance complicates cell guidance control over long distances. Our working hypothesis is that neuronal cells migrate directionally on an anisotropic and periodic scaffold. We tested this in a model involving neuronal PC12 cells cultured in a ratchet wheel-shaped microchamber. The microchamber was constructed by printing a patterned, thin film of polydimethylsiloxane (PDMS), to which the cells adhere weakly, onto a collagen-coated dish, to which the cells adhere strongly, using a microcontact printing technique. The cells can attach, extend neurites, and migrate on the anisotropic and periodic collagen-coated area between the ratchet wheel-shaped outer frame and round inner frame of the PDMS. We found that the microchamber geometry affected the direction of migration, even though the mean length of the longest neurite was independent of microchamber geometry. The time-course trace of cell body migration and neurite tips showed that the neurite tips remained around the tips of the ratchet teeth. These results suggested that neuronal cells migrate directionally on a scaffold, even in the absence of chemokine, and reveal a new conceptual framework for neuronal migration.
- 社団法人日本生物工学会の論文
著者
-
SUGAWARA Tadashi
Department of Basic Science, Graduate School of Arts and Science, The University of Tokyo
-
ASASHIMA Makoto
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
-
Ohnuma Kiyoshi
Univ. Tokyo
-
Ohnuma Kiyoshi
Department Of Physics School Of Science Nagoya University
-
Toyota Taro
Department of Basic Science, Research Center for Complex Systems Biology, Graduate School of Arts an
-
Toyota Taro
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering Chiba University:de
-
Toyota Taro
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering Chiba University
-
Asashima Makoto
Department Of Life Sciences (biology) Graduate School Of Arts And Sciences The University Of Tokyo:i
-
Ariizumi Takashi
Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo
-
Ariizumi Takashi
Department Of Life Sciences (biology) Graduate School Of Arts And Sciences The University Of Tokyo
-
Sugawara Tadashi
Department Of Basic Science Graduate School Of Arts And Sciences The University Of Tokyo
-
Ariizumi Takashi
Department Of Life Science (biology) Graduate School Of Arts And Sciences The University Of Tokyo
-
Asashima Makoto
Department Of Biological Science Graduate School Of Science University Of Tokyo
-
Sugawara Tadashi
Department Of Basic Science Graduate School Of Arts & Sciences The University Of Tokyo
-
Ohnuma Kiyoshi
Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo
関連論文
- Indication of Tunneling State of Hydrogen Atom in Hydrogen-Bonded Material 5-Bromo-9-hydroxyphenalenon Studied by X-ray and Neutron Diffractions(Condensed matter: structure and mechanical and thermal properties)
- Phase Transition Scheme of Isolated Hydrogen-bonded Material h-MeHPLN Studied by Neutron and X-ray Diffraction(Condensed Matter : Structure, Mechanical and Thermal Properties)
- Screening for novel pancreatic genes from in vitro-induced pancreas in Xenopus
- 1P-181 遠心沈降法によりジャイアントベシクルに内包されたカタラーゼの振動反応(生体膜・人工膜-構造・物性,第47回日本生物物理学会年会)
- 1P-180 遠心沈降によるジャイアントベシクル融合(生体膜・人工膜-構造・物性,第47回日本生物物理学会年会)
- Cloning and expression pattern of Xenopus prx-1 (Xprx-1) during embryonic development
- Expression of BMP-2,4 Genes during Early Development in Xenopus
- Facilitation of neurotransmitter release at the spiny lobster neuromuscular junction
- The Zoological Society of Japan 125th Anniversary SymposiumPROCEEDING OF THE 75^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
- Negative Magneto-resistance Observed on an Ion-radical Salt of a TTF-based Spin-polarized Donor
- Cloning a novel developmental regulating gene, Xotx5 : Its potential role in anterior formation in Xenopus laevis
- Evidence that far infrared radiation promotes growth of Xenopus laevis
- Immunocytochemical study of activin type IB receptor (XALK4) in Xenopus oocytes
- Global and temporal regulation of gene expression in Xenopus kidney cells in response to presumed microgravity generated by 3D clinostats
- Restricted Expression of Xenopus Midkine Gene during Early Development
- Synthesis and release of activin and noggin by cultured human amniotic epithelial cells
- The Na^+, K^+-ATPase α subunit requires gastrulation in the Xenopus embryo
- Role of the thrombopoietin (TPO)/Mpl system : c-Mpl-like molecule/TPO signaling enhances early hematopoiesis in Xenopus laevis
- Characterization of a gene respondent to clinorotation in Xenopus A6 cells
- Effects of rat Axin domains on axis formation in Xenopus embryos
- Quantification of Structural Properties of Cell-Sized Individual Liposomes by Flow Cytometry(MISCELLANEOUS)
- Blood Cell and Vessel Formation Following Transplantation of Activin-Treated Explants in Xenopus(Molecular and Cell Biology)
- Induction of Cells Expressing Vascular Endothelium Markers from Undifferentiated Xenopus Presumptive Ectoderm by Co-treatment with Activin and Angiopoietin-2(Developmental Biology)
- In vitro induction of the pronephric duct in Xenopus explants
- ROLE OF CXCR4/SDF-1 SIGNALING IN XENOPUS GASTRULATION(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- MODIFICATION OF GENE EXPRESSION BY ACTIVIN-A IN XENOPUS PRESUMPTIVE ECTODERM(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- 2P250 セルソーターを用いた巨大ベシクルの融合検出(生体膜・人工膜-ダイナミクス,第48回日本生物物理学会年会)
- 3P231 細胞サイズリポソーム内での分子演算反応(生体膜・人工膜-ダイナミクス,第48回日本生物物理学会年会)
- Midkine Counteracts the Activin Signal in Mesoderm Induction and Promotes Neural Formation^1
- Hydrogen-Bond Nature Studied by X-ray and Neutron Structure Analyses of MeHPLN at Room Temperature
- Characterization of myeloid cells derived from the anterior ventral mesoderm in the Xenopus laevis embryo
- Incommensurate-Commensurate Phase Transition of Intramolecular Hydrogen-Bonded System : 5-Bromo-9-Deuteroxyphenalenone (d-BrHPLN)
- 2P-139 異方的周期構造の微細パターンにおける細胞運動のマイクロ干渉イメージング(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- Directional migration of neuronal PC12 cells in a ratchet wheel shaped microchamber(CELL AND TISSUE ENGINEERING)
- INDUCTION OF MAXILLOFACIAL CARTILAGE FROM XENOPUS PRESUMPTIVE ECTODERM BY ACTIVIN A(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- INDUCTION OF BLOOD VESSELS FROM UNDIFFERENTIATED XENOPUS EXTODERM (ANIMAL CAP) BY ACTIVIN A(Taxonomy and Systematics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- EFFECT OF TGF-β ON MORPHOGENESIS IN RAT SALIVARY GLAND-DERIVED RSMG-1 CELLS
- Endoderm differentiation and inductive effect of activin-treated ectoderm in Xenopus
- Cytochalasin B inhibits morphogenetic movement and muscle differentiation of activin-treated ectoderm in Xenopus
- Neural induction in embryos
- Patterns of gene expression in the core of Spemann's organizer and activin-treated ectoderm in Cynops pyrrhogaster
- Activin-treated ectoderm has complete organizing center activity in Cynops embryos
- Isotope Effect on the Dielectric Phase Transitions of 5-Bromo-9-Hydroxyphenalenone
- IN VITRO INDUCTION AND TRANSPLANTATION OF EYE DURING EARLY XENOPUS DEVELOPMENT(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- In vitro induction and transplantation of eye during early Xenopus development
- IN VITRO INDUCTION AND TRANSPLANTATION OF EYEBALL DURING EARLY XENOPUS DEVELOPMENT(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Electron Microscopical and Histochemical Studies of the Spontaneous Tumors of Xenopus laevis : Cell Biology
- COMPARISON OF INDUCTION DURING DEVELOPMENT BETWEEN XENOPUS TROPICALIS AND XENOPUS LAEVIS(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Determination, induction and pattern formation in early amphibian embryos
- Sequential gene expression durnig pronephric tubule formation in vitro in Xenopus ectoderm
- Tumors in Amphibia
- Xenopus tropicalis as an ideal laboratory animal of Amphibia
- ESTABLISHMENT OF RSMG-2 CELL LINE DERIVED FROM MALE RAT SUBMANDIBULAR GLAND IN SERUM-FREE DEFINED CULTURE
- Charge Transfer Assisted Ferromagnetic Coupling Observed in an Ion-Radical Salt of a Cross-Cyclophane TTF-Based Donor
- Three-Dimensional Sulfur-Based π-Donor: A Novel Dimeric TTF Derivative of a Cross-Cyclophane Type
- Chromosome mapping of Xenopus tropicalis using the G- and Ag-bands : Tandem duplication and polyploidization of larvae heads
- Cloning and characterization of the Xenopus laevis p8 gene
- TWO DISTINCT ROLES OF PCP GENES IN XENOPUS GASTRULATION(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Ground State Triplet Cation Diradicals Generated from N, N-Dimethylamino Nitronyl Nitroxide and Its Homologues through One-Electron Oxidation
- Structure and Physical Properties of Two Perchlorate Salts Derived from a Novel Twin Donor. Bis(4', 5'-ethylenedithio-5-methylthiotetrathiafulvalenyl-4-thio)methane
- Listeria-like Motion of Oil Droplets
- Morphological Change of Giant Vesicles Triggered by Dehydrocondensation Reaction
- Effects of High and Very Low Magnetic Fields on the Growth of Hairy Roots of Daucus carota and Atropa belladonna
- Changes in the adhesive properties of dissociated and reaggregated Xenopus laevis embryo cells
- Onset of competence to respond to activin A in isolated eight-cell stage Xenopus animal blastomeres
- Claudin5 genes encoding tight junction proteins are required for Xenopus heart formation
- 2P471 A liposome containing an autonomous DNA computing system inside(50. Non-equilibrium and complex system,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Magnetic Property of 1,3,5-Tris(4',4',5',5'-tetramethylimidazolin-2'-yl)benzene 3',3'',3'''-Trioxide 1',1'',1'''-Trioxyl in 1 : 1 Mixed Crystals with 1,3,5-Trinitrobenzene
- Helical Aggregate of Oleic Acid and Its Dynamics in Water at pH 8
- Crystal Structure of Buckminsterfullerene (C_) Incorporated by a U-Shaped Twin Donor
- Regulation of the Xmyf-5 and XmyoD Expression Pattern during Early Xenopus Development
- Development of Ca^ signaling mechanisms and cell motility in presumptive ectodermal cells during amphibian gastrulation
- I-2 EMBYROGENESIS OF ALIMENTARY TRACT DUPLICATION AND ENTERIC CYST : CONSIDERATION OF SPLIT NOTOCHORD SYNDROME
- Transformation between Monovalent and Divalent Ionic Solids : An Ionic(I)-Ionic(II) Phase Transition in a Biferrocene-F_1TCNQ Charge-Transfer Complex(Cross-disciplinary Physics and Related Areas of Science and Technology)
- Chemical Consequences of Arylnitrenes in the Crystalline Environment
- Preparation and Characterization of Gold Nano-Particles Chemisorbed by π-Radical Thiols
- Tautomerizaton of 2-Carboxy-1,3-dibenzo[a,c]tropolone in the Solid State. Possibility of a Symmetrical Structure of the Enol Form
- 1P-190 高頻度に分裂するベシクル自己複製系の構築とマイクロチャンバーを用いたその形態変化ダイナミクス計測(生体膜・人工膜-ダイナミクス,第47回日本生物物理学会年会)
- Formation of Self-Assembled Monolayer of Phenylthiol Carring Nitronyl Nitorxide on Gold Surface
- Multifunctionality of the Activin Molecule(Messages from Comparative Endocrinology: Diverse Hormone Function,Symposium,PROCEEDING OF THE 75^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
- A CELL CYCLE REGULATOR, XBtg2 IS REQUIRED FOR XENOPUS NEURAL DEVELOPMENT(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- History and Current Role of the Academic Journal of the Zoological Society of Japan : From Zool. Mag. and Annat. Zool. to Zoological Science(Symposium On the 20^ Anniversary of Publishing Zoological Science,PROCEEDING OF THE 74^ ANNUAL MEETING OF
- 2P-162 新規細胞モデルとしての分子コンピュータ封入ベシクル(生体膜・人工膜-構造・物性,第47回日本生物物理学会年会)
- Temporal Emergence of Giant Vesicles Accompanied by Hydrolysis of Ammonium Amphiphiles with a Schiff-base Segment
- Embryonic Induction and Cell Diffierentiation During the Amphibian Development : THE ZOOLOGICAL SOCIETY PRIZE
- Control of the Embryonic Body Plan by Activin during Amphibian Development
- Volatile Plant Constituents Cause Behavioural Changes Ultimately Leading to Death in the Newt (Cynops pyrrhogaster)
- Impurity Resistivity in Platinum
- Identification and characterization of Xenopus OMP25
- In vitro control of organogenesis and fundamental embryonic form by the peptide growth factor activin
- Successive Fusion of Vesicles Aggregated by DNA Duplex Formation in the Presence of Triton X-100
- Development of the embryonic kidney
- Transformation between Monovalent and Divalent Ionic Solids : An Ionic(I)-Ionic(II) Phase Transition in a Biferrocene-F_1TCNQ Charge-Transfer Complex(Cross-disciplinary Physics and Related Areas of Science and Technology)
- Small-Angle X-Ray Scattering Method to Determine the Size Distribution of Gold Nanoparticles Chemisorbed by Thiol Ligands
- Morphological changes in newt epitheliomas caused by controlled temperature.
- Anomeric Effect in Sulfur Heterocycles Carrying Sulfur Substituents
- Spontaneous Progressive Skin Papilloma in (NewtsCynops pyrrhogaster)
- Histological and electron microscopical studies on neoplasia subcutaneously occurring in Xenopus laevis.
- Giant Vesicle Formation of Novel Polymerizable Amphiphile Associated with Its Polymerization and Hydrolysis in Water
- Giant Vesicles Containing Microspheres with High Volume Fraction Prepared by Water-in-oil Emulsion Centrifugation