Expression of the Xylan-Degrading Genes of Bacillus pumilus IPO in Saccharomyces cerevisiae

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概要

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• Xylanase(xynA)and β-xylosidase(xynB)genes of Bacillus pumilus were expressed in Saccharomyces cerevisiae by using the GAP(glyceraldehyde-3-phosphate dehydrogenase)promoter of S. cerevisiae. Yeast cells harboring a plasmid pNAX2 containing xynA produced xylanase in the cytoplasm of the cell to an extent as much as 5% of the total soluble protein in the cell extract. Xylanase produced in yeast had an extra methionine at the N-terminus, but had the same specific activity as that produced by B. pumilus IPO. The xylanase in the yeast was not glycosylated and was immunologically identical to that of B. pumilus IPO. Yeast cells harboring a plasmid pYXB containing xynB produced β-xylosidase in the cytoplasm of the cell(3% of the total soluble protein). β-Xylosidase purified from the yeast strain exhibited specific activity nearly equal to the value of enzyme purified from B. pumilus, and had an N-terminal sequence identical to the sequence of the enzyme from B. pumilus.

• 社団法人日本生物工学会の論文
• 1991-05-25

著者

• Negoro Seiji

  Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo

• Negoro Seiji

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Negoro Seiji

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

• Urabe I

  Osaka Univ. Osaka Jpn

• Urabe Itaru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Urabe I

  Graduate School Of Engineering Osaka University

• Urabe Itaru

  Department Of Biotechnology Faculty Of Engineering Osaka University

• OKADA HIROSUKE

  (Present address)Department of Applied Microbial Technology, The Kumamoto Institute of Technology

• Urabe Itaru

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Xu W‐z

  Osaka Univ. Osaka Jpn

• Xu Wei-zhong

  Department Of Biotechnology Faculty Of Engineering Osaka University

• PAN CAI-XI

  Department of Fermentation Technology, Osaka University

• AKATSUKA HIROYUKI

  Department of Fermentation Technology, Osaka University

• SHIMA YASUFUMI

  Department of Fermentation Technology, Osaka University

• Negoro Seiji

  Department Of Biotechnology Osaka University

• Okada H

  Kansai Univ. Osaka Jpn

• Okada H

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• Okada H

  Nagaoka Univ. Technol. Niigata Jpn

• Okada H

  Nagoya City Univ. School Of Medicine Nagoya Jpn

• Okada H

  Department Of Fermentation Technology Facutly Of Enginering Osaka Univeristy

• Urabe Itaru

  Department Of Biotechnology Osaka University

• Pan Cai-xi

  Department Of Fermentation Technology Osaka University

• Shima Yasufumi

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• AKATSUKA HIROYUKI

  Discovery Research Laboratory, Tanabe Seiyaku Co.Ltd.

• Akatsuka Hiroyuki

  Discovery Research Laboratory Tanabe Seiyaku Co.ltd.

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