Purification and Characterization of Alkylcatechol 2,3-Dioxygenase from Butylphenol Degradation Pathway of Pseudomonas putida MT4(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
- 論文の詳細を見る
Alkylcatechol 2, 3-dioxygenase was purified from the cell extract of recombinant Escherichia coli JM109 harboring the alkylcatechol 2, 3-dioxygenase gene (bupB) cloned from the butylphenol-degrading bacterium Pseudomonas putida MT4. The purified enzyme (BupB) showed relative meta-cleavage activities for the following catechols : catechol (100%), 4-methylcatechol (572%), 4-n-butylcatechol (185%), 4-n-hexylcatechol (53%), 4-n-heptylcatechol (45%), 4-n-nonylcatechol (10%), 4-tert-butylcatechol (0%), and 3-methylcatechol (33%). The kinetic parameters, namely, K_m and V_<max>, for catechol, 4-methylcatechol, and 4-n-butylcatechol, were 23.4, 8.4, and 6.5μM and 25.8, 76.9, and 18.0U mg^<-1>, respectively. These results suggest that BupB has broad substrate specificity for 4-n-alkylcatechols.
- 社団法人日本生物工学会の論文
- 2007-10-25
著者
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Takeo Masahiro
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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Kato Dai-ichiro
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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Negoro Seiji
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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Negoro Seiji
Department Of Materials Science And Chemistry Graduate School Of Engineering University Of Hyogo
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Negoro Seiji
Department Of Biotechnology Faculty Of Engineering Osaka University
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Takeo Masahiro
Department Of Materials Science And Chemistry Graduate School Of Engineering University Of Hyogo
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Takeo Masahiro
Department Of Applied Chemistry Himeiji Institute Of Technology
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KITAMURA Chitoshi
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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Negoro Seiji
Graduate School Of Engineering Univ. Of Hyogo
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Nishimura Munehiro
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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Takahashi Hana
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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Takeo Masahiro
兵庫県立大学 工学研究科物質系工学専攻
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Negoro Seiji
兵庫県立大学 工学研究科物質系工学専攻
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Takahashi Hana
Department Of Materials Science And Chemistry Graduate School Of Engineering University Of Hyogo
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Kitamura Chitoshi
Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec
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Kato Dai-ichiro
Graduate School Of Engineering University Of Hyogo
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Kato Dai-ichiro
Department Of Materials Science And Chemistry Graduate School Of Engineering University Of Hyogo
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Nishimura Munehiro
Department Of Materials Science And Chemistry Graduate School Of Engineering University Of Hyogo
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