Cloning and Characterization of a 4-Nitrophenol Hydroxylase Gene Cluster from Rhodococcus sp. PN1

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概要

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• A 4-nitrophenol (4-NP)-degrading bacterium was isolated from activated sludge and identified as a Rhodococcus sp. This bacterium, designated as strain PN1, could utilize 4-NP as a sole carbon, nitrogen and energy source. Degradation tests of 4-NP using cell suspensions of strain PN1 revealed that the degradation was induced by 4-NP and that 4-nitrocatechol (4-NC) was one of the metabolites. A gene library was constructed from the total DNA of strain PN1 and introduced into Rhodococcus rhodochrous ATCC 12674. Two recombinant strains showed 4-NP hydroxylase activity, and a 9.1-kb DNA fragment encoding the activity was isolated from one of the strains. In addition, a 2.4-kb smaller fragment expressing the activity was subcloned from the 9.1-kb fragment and sequenced. The sequence analysis showed that the fragment encodes a two-component 4-NP hydroxylase, the predicted amino acid sequence of which exhibits significant similarity to those of phenol hydroxylases and 4-hydroxyphenylacetate 3-hydroxylases belonging to the two-component flavin diffusible monooxygenase (TC-FDM) family proposed by Galan et al. (J. Bacteriol., 182, 627-636, 2000).

• 社団法人日本生物工学会の論文
• 2003-02-25

著者

• Takeo Masahiro

  Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo

• Negoro Seiji

  Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo

• Negoro Seiji

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Negoro Seiji

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

• Takeo M

  Himeji Inst. Technol. Hyogo Jpn

• Takeo Masahiro

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

• Takeo Masahiro

  Dept.of Applied Chemistry Himeji Institute Of Technology

• Takeo Masahiro

  Department Of Applied Chemistry Himeji Institute Of Technology

• Takeo Masahiro

  Department Of Applied Chemistry Himeiji Institute Of Technology

• MAEDA YOSHIMICHI

  Department of Applied Chemistry, Himeji Institute of Technology

• Maeda Yoshimichi

  Department Of Applied Chemistry Faculty Of Engineering Himeji Institute Of Technology

• Negoro Seiji

  Department Of Biotechnology Osaka University

• ABE YOSHIKATSU

  Department of Materials Science and Chemistry, Graduate School of Engineering, Himeji Institute of T

• YASUKAWA TAKESHI

  Department of Applied Chemistry, Himeji Institute of Technology

• NIIHARA SANAE

  Department of Applied Chemistry, Himeji Institute of Technology

• Niihara Sanae

  Department Of Applied Chemistry Himeji Institute Of Technology

• Abe Yoshikatsu

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

• Yasukawa Takeshi

  Department Of Anesthesiology And Icm Kawasaki Medical School

• Yasukawa Takeshi

  Department Of Applied Chemistry Himeji Institute Of Technology

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