Characterization of Endo-Type 6-Aminohexanoate-Oligomer Hydrolase from Flavobacterium sp.

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概要

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• Cyclic oligomers of 6-aminohexanoate were fractionated from waste materials obtained from a nylon-6 factory by Sephadex LH-20 gel filtraitoin column chromatography, and two major fractions, a cyclic tetramer and cyclic dimer, were obtained. The 6-aminohexanoate-oligomer hydrolase (EIII) from Flavobacterium sp. KI72 was active toward the cyclic tetramer, but not toward the cyclic dimer. The M_r of the EIII enzyme was estimated to be 93,000 by high performance gel filtration chromatography (TSK-G3000SW), while that of the polypeptide as deduced from the nucleotide sequence was calculated to be 36,902,suggesting that the EIII enzyme has a dimeric or trimeric structure. The enzyme was stable between the pH 6.5 and 11 with an optimum pH of 7.0. The enzyme activity was stable up to 45℃ with an optimal temperature of 40℃.

• 社団法人日本生物工学会の論文
• 1995-07-25

著者

• Negoro Seiji

  Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo

• Negoro Seiji

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Negoro Seiji

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

• Urabe I

  Osaka Univ. Osaka Jpn

• Urabe Itaru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Urabe I

  Graduate School Of Engineering Osaka University

• Urabe Itaru

  Department Of Biotechnology Faculty Of Engineering Osaka University

• OKADA HIROSUKE

  (Present address)Department of Applied Microbial Technology, The Kumamoto Institute of Technology

• Urabe Itaru

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Negoro Seiji

  Department Of Biotechnology Osaka University

• Okada H

  Kansai Univ. Osaka Jpn

• Okada H

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• Okada H

  Nagaoka Univ. Technol. Niigata Jpn

• Okada H

  Nagoya City Univ. School Of Medicine Nagoya Jpn

• Okada H

  Department Of Fermentation Technology Facutly Of Enginering Osaka Univeristy

• Urabe Itaru

  Department Of Biotechnology Osaka University

• KAKUDO SHINJI

  Department of Biotechnology, Osaka University

• Urabe Itaru

  Graduate School Of Engineering Osaka University

• Kakudo S

  Department Of Biotechnology Osaka University

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