Simple Detection of Xylosidase Activity in Single Colonies, Using 1-Naphthyl-β-D-xylopyranoside
スポンサーリンク
概要
- 論文の詳細を見る
Since the xylosidase of Bacillus pumilus hydrolyzed 1-naphthyl-β-D-xylopyranoside (naphthyl-X) to produce xylose and 1-naphthol and a chromogenic azo compound is produced by coupling 1-naphthol and Fast Blue Salt B, a simple method for detection of xylosidase activity in single colonies was studied. Escherichia coli JM109 carrying the xylosidase gene of B. pumilus was cultivated at 37℃ for 18 h on an LB plate containing 0.5 mg/ml naphthyl-X, and then the plate was overlaid with 3 ml of a top layer containing 24 mg of agar and 6mg of Fast Blue Salt B. After incubation of the plate at 37℃ for 1 h, each colony became reddish-brown. Even a small colony with the xylosidase on the plate was easily distinguished from colonies without the enzyme.
- 社団法人日本農芸化学会の論文
- 1996-09-23
著者
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Taguchi Hisataka
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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Akamatsu Takashi
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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Okada H
Kansai Univ. Osaka Jpn
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Okada H
Department Of Fermentation Technology Faculty Of Engineering Osaka University
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Okada H
Nagaoka Univ. Technol. Niigata Jpn
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Okada H
Nagoya City Univ. School Of Medicine Nagoya Jpn
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Okada H
Department Of Fermentation Technology Facutly Of Enginering Osaka Univeristy
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Okada Hirosuke
Department Of Fermentation Technology Faculty Of Engineering Osaka University
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Okada Hirosuke
Deparment Of Fermentation Technology Osaka University
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Akamatsu Takashi
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Taguchi H
Department Of Bioresource Development Hiroshima Prefectural University
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Akamatsu T
Gifu Univ. Gifu Jpn
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Maeda Kazue
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Hamasaki T
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Akamatsu T
Sojo Univ. Kumamoto Jpn
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HAMASAKI Tohru
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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Hamasaki T
Muroran City General Hospital
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Taguchi Hisataka
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Hamasaki T
Hokkaido Social Insurance Hospital
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