Ethanol production from D-lactic acid by lactic acid-assimilating Saccharomyces cerevisiae NAM34-4C(BREWING AND FOOD TECHNOLOGY)
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概要
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The lactic acid-assimilating yeast Saccharomyces cerevisiae NAM34-4C grew rapidly in minimal D-lactate medium (pH 3.5) at 35℃, compared with minimal L-lactate medium. A laboratory strain, S. cerevisiae S288C, did not grow in either medium at pH 3.5. Strain NAM34-4C produced remarkably high levels of ethanol in YPDL medium at pH 3.5, but not at pH 5.5, when D-lactate was provided as the carbon source. Optimal cultivation conditions for ethanol production from D-lactate by strain NAM34-4C were as follows: shaking speed, 60 rpm; initial pH, 3.0; cultivation temperature, 35℃; yeast extract, 5 g/L; peptone, 10 g/L; and D-lactate, 30 g/L. Under these conditions, strain NAM34-4C produced 2.7 g/L ethanol, which is 18% of the theoretical maximal yield (0.51 × initial D-lactate concentration).
- 2013-07-00
著者
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Kida Kenji
Graduate School Of Science And Technology Kumamoto University
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Matsuoka Masayoshi
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Akamatsu Takashi
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Taguchi Hisataka
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Wakamatsu Makoto
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University
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Tani Tatsunori
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University
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