Accumulation of a Recombinant Aspergillus oryzae Lipase Artificially Localized on the Bacillus subtilis Cell Surface
スポンサーリンク
概要
- 論文の詳細を見る
cutL cDNA encoding awn extracellular lipase, L1, /from Aspergillus oryzae was fused to the cell wall-binding domain (CWB) region of a plasmid, pHCB3R. SDS-polyacrylamide gel electrophoresis (PAGE) and zymography of proteins extracted from the cell surface of Bacillus subtilis 168 harboring a fused lipase plasmid (pHCB3RCL) revealed that the fused gene product, CWB-CutL, was localized in the B. subtilis cell wall and retained lipase activity. B. subtilis WASD (wprA sigD), recently used for the accumulation of CWB-LipB (the CWB protein fused with B. subtilis lipase B), was also a suitable host for the accumulation of CWB-CutL, the amount being 10% of the total proteins extracted from the cell surface.
- 社団法人日本生物工学会の論文
- 2000-10-25
著者
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Akamatsu Takashi
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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YAMAMOTO Hiroki
Department of Biochemistry, Osaka University of Pharmaceutical Sciences
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SHIDA Toshio
Department of Applied Biology Faculty of Textile Science and Technology, Shinshu University
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Sekiguchi Junichi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Akamatsu Takashi
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Akamatsu Takashi
Department Of Applied Microbiology And Technology Sojo University
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Shida T
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Shida Toshio
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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YAMAMOTO Hiroyuki
Institute of High Polymer Research, Faculty of Textile Science and Technology, Shinshu University
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KOBAYASHI GOTA
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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TOIDA JUNICHI
Food Technology Research Institute of Nagano Prefecture
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Sekiguchi J
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Kobayashi Gota
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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TOIDA Jinichi
Food Technology Research Institute of Nagano Prefecture
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Yamamoto Hiroyuki
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Yamamoto Hiroki
Department Of Agro-environmental Sciences Graduate School Of Bioresource And Bioenvironmental Scienc
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