Simultaneous Display of Bacterial and Fungal Lipases on the Cell Surface of Bacillus subtilis
スポンサーリンク
概要
- 論文の詳細を見る
A small cell wall-binding domain (CWB_c) of the Bacillus subtilis peptidoglycan hydrolase CwlC fused to B.subtilis lipase B was able to be localized on the cell wall of B.subtilis. With the aim of developing an efficient lipid-hydrolyzing bacterium with two different lipases on the cell surface, plasmids possessing genes for LipB-CWB_c and CWB_b-CutL (the cell wall-binding domain of CwlB fused to the Aspergillus oryzae lipolytic enzyme CutL) were constructed. B.subtilis harboring these plasmids accumulated considerable amounts of both lipases on the cell surface.
- 公益社団法人日本生物工学会の論文
- 2002-01-25
著者
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Fujii Kaori
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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YAMAMOTO Hiroki
Department of Biochemistry, Osaka University of Pharmaceutical Sciences
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Serizawa Masakuni
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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YAMAMOTO Hiroyuki
Institute of High Polymer Research, Faculty of Textile Science and Technology, Shinshu University
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KOBAYASHI GOTA
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Sekiguchi J
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Kobayashi Gota
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Yamamoto Hiroyuki
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Yamamoto Hiroki
Department Of Agro-environmental Sciences Graduate School Of Bioresource And Bioenvironmental Scienc
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