Transformation of Penicillium urticae with Plasmids Containing the Hygromycin B Resistance Gene(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
A transformation system with efficiencies between 6 and 59 stable transformants per μg of DNA was developed for Penicillium urticae J1 (ATCC48163) using hygromycin B-resistant plasmids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random integration of vector DNA were observed. Although P. uricae was unable to grow in the presence of 200 μg/ml hygromycin B, the transformants were resistant to more than 5mg/ml of hygromycin B.
- 社団法人日本農芸化学会の論文
- 1991-12-23
著者
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YAMAMOTO Hiroki
Department of Biochemistry, Osaka University of Pharmaceutical Sciences
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Yamamoto H
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sekiguchi Junichi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Yamamoto H
Department Of Developmental Biology And Neurosciences Graduate School Of Life Sciences Tohoku Univer
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NARUSE Atsushi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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KIUCHI Naomi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Kiuchi Naomi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Naruse Atsushi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Yamamoto H
Department Of Mechanical Engineering Doshisha University
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Sekiguchi J
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Yamamoto Hiroki
Department Of Agro-environmental Sciences Graduate School Of Bioresource And Bioenvironmental Scienc
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