Intraspecific Hybridization of a Methanol-utilizing Yeast, Candida sp. N-16, through Protoplast Fusion(Microbilolgy & Fermentation Industry)

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概要

• 論文の詳細を見る

• Auxotrophic mutants of a methanol-utilizing yeast, Candida sp. N-16, were hybridized through protoplast fusion using polyethylene glycol and Ca^<2+> ions. The complementation frequency of fusion was about 2 to 5×10^<-4>. Some of the prototrophic fusants obtained were very stable and distinguishable from the original prototroph. The fusants were uninuclear and contained 3.8 to 4.2 mg of DNA per 10^<11> cells as compared with about 1.5mg of DNA in the parental strains. The cell volume of the fusants was greater than that of the wild and parental strains. The growth rate of the fusants was slightly lower than that of the wild strain. There was a difference between the fusants and the wild strain with respect to the levels of alcohol-dissimilating enzymes in cells grown on methanol, and one of the fusants possessed about 2.5 times higher activity of alcohol oxidase than that of the wild strain.

• 社団法人日本農芸化学会の論文
• 1988-07-23

著者

• ANDO Akikazu

  Department of Biotechnology, Graduate School of Science and Technology, Chiba University

• YAMAMOTO Hideki

  Department of Surgery, Tama-Nagayama Hospital, Nippon Medical School

• FUJII TAKAAKI

  Department of Bioresources Science, Graduate School of Science and Technology, Chiba University

• Yamamoto H

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• YABUKI Minoru

  Department of Agricultural Chemistry, Chiba University

• Ando Akikazu

  Department Of Agricultural Chemistry Chiba University

• Yabuki Minoru

  Department Of Agricultural Chemistry Chiba University

• Fujii T

  Department Of Agricultural Chemistry Chiba University

• Yamamoto Hideki

  Deparment Of Toxicology Faculty Of Veterinary Medicine Hokkaido University

• Yamamoto H

  Department Of Developmental Biology And Neurosciences Graduate School Of Life Sciences Tohoku Univer

• FUJINAMI Kazuko

  Department of Agricultural Chemistry, Chiba University

• TAKENAKA Eiji

  Department of Agricultural Chemistry, Chiba University

• Takenaka Eiji

  Department Of Agricultural Chemistry Chiba University

• Fujinami Kazuko

  Department Of Agricultural Chemistry Chiba University

• Yamamoto H

  Department Of Mechanical Engineering Doshisha University

• Fujii Takaaki

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• FUJII Takaaki

  Department of Agricultural Chemistry, Chiba University

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