Purification and Properties of Amylases Extracellularly Produced by an Imperfect Fungus, Fusidium sp. BX-1 in a Glycerol Medium

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概要

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• Amylases (I and II) extracellularly produced by an imperfect fungus, Fusidium sp. BX-1 in a medium containing glycerol as a carbon source, were purified as electrophoretically and isoelectrophoretically homogeneous proteins. The electrophoretical mobilities of amylase I and II on native and SDS-polyacrylamide gels exactly coincided with each other. Their molecular weights were estimated to be about 52,000. The sugar contents of amylase I and II were 3.4 and 4.7%, and the pIs were 8.70 and 8.55,respectively. The K_ms of the enzymes for soluble starch were 0.053 and 0.044%. The actions of the enzymes on soluble starch, short chain amyloses, and maltose were examined. Amylase I and II are identified as being to an α-amylase and a glucoamylase, respectively.

• 社団法人日本農芸化学会の論文
• 1992-03-23

著者

• OHNO Nobuko

  Department of Medicine, University of Pennsylvania School of Medicine

• ANDO Akikazu

  Department of Biotechnology, Graduate School of Science and Technology, Chiba University

• Fujii T

  Kyoto Univ. Kyoto

• SHINOYAMA HIROFUMI

  Department of Bioresources Science, Graduate School of Science and Technology, Chiba University

• FUJII TAKAAKI

  Department of Bioresources Science, Graduate School of Science and Technology, Chiba University

• Ohno Nobuko

  Department Of Health And Nutrition School Of Home Economics Wayo Woman's University

• Ohno Nobuhiro

  Department Of Health And Nutrition School Of Home Economics Wayo Woman's University

• Ando A

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Ando Akikazu

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Ando Akikazu

  Department Of Agricultural Chemistry Chiba University

• IJUIN Taeko

  Faculty of Literature and Home Economics, Wayo Women's University

• SONG Suk

  Department of Bioproduction Science, Faculty of Horticulture, Chiba University

• UCHIYAMA Shigeru

  Department of Bioproduction Science, Faculty of Horticulture, Chiba University

• Song Suk

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Ijuin Taeko

  Faculty Of Literature And Home Economics Wayo Women's University

• Uchiyama S

  Banyu Pharmaceutical Co. Ltd. Ibaraki Jpn

• Shinoyama H

  Department Of Advanced Bioresources Science Graduate School Of Science And Technology Chiba Universi

• Shinoyama Hirofumi

  Department Of Agricultural Chemistry Chiba University

• Fujii Takaaki

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Uchiyama S

  Gunma Univ. Gunma Jpn

• FUJII Takaaki

  Department of Agricultural Chemistry, Chiba University

• Shinoyama Hirofumi

  Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University

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