Isolation and Properties of Malic Enzyme and Its Gene in Rhodopseudomonas palustris No. 7
スポンサーリンク
概要
- 論文の詳細を見る
Malic enzyme (ME) was purified as an electrophoretically homogenous protein from Rhodopseudomonas palustris No. 7. The molecular weight of ME was estimated to be 650 kDa and that of its subunit, 86 kDa. ME activity was remarkably enhanced by di- and mono-valent cations, and the Ka values for Mg2+ and NH4+ were 0.26 and 0.56 mM respectively. Purified ME used both NAD+ and NADP+ as electron acceptors, with Km values of 0.11 and 1.8 mM. The Km value for L-malate was 1.7 mM using NAD+ as electron acceptor. Gene cloning of the ME indicated that the ME from R. palustris strain No. 7 was composed of 774 amino acids encompassing the ME and phosphotransacetylase domains, although purified ME displayed no phosphotransacetylase activity. ME activity was inhibited by acetyl-CoA, oxaloacetate, and fructose-6-phosphate. These results suggest that ME plays an important role in the metabolic regulation of R. palustris No. 7 under photoheterotrophic conditions.
- 社団法人 日本農芸化学会の論文
- 2010-01-23
著者
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Fujii T
Kyoto Univ. Kyoto
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Fujii T
Department Of Applied Biological Chemistry The University Of Tokyo
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Fujii Takeshi
National Institute for Agro-Environmental Sciences
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Amachi Seigo
Div. Of Bioresource Sci. Graduate School Of Horticulture Chiba Univ.
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Fujii T
Div. Of Bioresource Sci. Graduate School Of Horticulture Chiba Univ.
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Yoshikawa Jun
Mesoscopic Materials Research Center Kyoto University
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Fujii T
Yamaguchi Univ. Yamaguchi Jpn
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Yoshikawa J
Division Of Bioresource Science Graduate School Of Horticulture Chiba University
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Amachi Seigo
Division Of Bioresource Science Graduate School Of Horticulture Chiba University
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SATO Ikuo
Division of Bioresource Science, Graduate School of Horticulture, Chiba University
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YOSHIKAWA Jun
Division of Bioresource Science, Graduate School of Horticulture, Chiba University
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FURUSAWA Akiko
Division of Bioresource Science, Graduate School of Horticulture, Chiba University
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CHIKU Kazuhiro
Division of Bioresource Science, Graduate School of Horticulture, Chiba University
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FUJII Takaaki
Division of Bioresource Science, Graduate School of Horticulture, Chiba University
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Yoshikawa Jun
Division Of Bioresource Science Graduate School Of Horticulture Chiba University
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Chiku Kazuhiro
Division Of Bioresource Science Graduate School Of Horticulture Chiba University
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Chiku Kazuhiro
Department Of Advanced Bioresource Science Graduate School Of Science And Technology
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Furusawa Akiko
Division Of Bioresource Science Graduate School Of Horticulture Chiba University
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Sato Ikuo
Division Of Bioresource Science Graduate School Of Horticulture Chiba University
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