2,4-Dichrolophenoxyacetic Acid-degrading Genes from Bacteria Isolated from Soil in Japan : Spread of Burkholderia cepacia RASC-type Degrading Genes Harbored on Large Plasmids

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Fourteen strains of 2,4-dichrolophenoxyacetic acid (2,4-D)-degrading bacteria were isolated from soil samples from eight different sites in Japan and identified as members of the genera Burkholderia, Cupriavidus, and Sphingobium. To characterize them in view of the 2,4-D-degrading genes that they had, the strains were divided into three groups based on similarities of PCR-amplified partial nucleotide sequences of their 2,4-D-degrading genes to the sequences of well-characterized 2,4-D-degrading genes, tfdA, tfdB, and tfdC in Cupriavidus necator strain JMP134, Achromobacter xylosoxidans strain EST4002, and Sphingomonas sp. strain TFD44. The nucleotide sequences of the tfdA, tfdB, and tfdC gene homologs in eight of the 14 strains were highly similar to those in Burkholderia cepacia strain RASC. The eight strains were classified into the RASC subgroup in the EST4002 group. Southern hybridization indicated that at least one set of the putative tfdA, tfdB, and tfdC genes in each of the 14 strains was located on a replicon (ca. 90-600 kb). In particular, the RASC-type 2,4-D-degrading genes of the eight strains were located on plasmids of similar size (ca. 600 kb), and the hybridization experiments suggested that the 2,4-D-degrading genes of seven strains among the eight were located on restriction fragments of the same length. In view of the fact that the seven strains originated from six distant sites in Japan and had different 16S rRNA gene sequences, our results suggest that the RASC-type 2,4-D-degrading genes on similar plasmids were horizontally transferred among bacteria and were distributed throughout Japan.
日本微生物生態学会の論文

2,4-Dichrolophenoxyacetic Acid-degrading Genes from Bacteria Isolated from Soil in Japan : Spread of Burkholderia cepacia RASC-type Degrading Genes Harbored on Large Plasmids

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