Induction and Repression of a Streptomyces lividans Chitinase Gene Promoter in Response to Various Carbon Sources
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概要
- 論文の詳細を見る
Induction and repression of a gene for chitinase (chiA) in Streptomyces lividans was investigated using a catechol 2,3-dioxygenase gene (xylE) as the reporter gene. Of various substrates examined, expression of the promoter (PchiA) was observed after a delay when colloidal chitin or small chitin-oligosaccharides were added to the medium. N-acetylglucosamine completely repressed the chiA promoter. The duration of the delay in expression of PchiA differed with the inducer used, with chitobiose inducing the activity most rapidly. The minimum concentration of chitobiose needed for induction was 1 μM. It appears, therefore, that an efficient inducer of the gene for chitinase in S. lividans is chitobiose.
- 社団法人日本農芸化学会の論文
- 2000-01-23
著者
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Fujii T
Kyoto Univ. Kyoto
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Fujii T
Department Of Applied Biological Chemistry The University Of Tokyo
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Saito Akiko
Biotechnology Center Toyama Prefecture
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Saito Akiko
Department Of Applied Biological Chemistry The University Of Tokyo
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Fujii Takeshi
National Institute for Agro-Environmental Sciences
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Fujii T
Inst. Biol. Sci. Univ. Tsukuba
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Fujii T
Div. Of Bioresource Sci. Graduate School Of Horticulture Chiba Univ.
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SAITO Akihiro
National Institute for Agro-Environmental Sciences
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MIYASHITA Kiyotaka
National Institute for Agro-Environmental Sciences
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Miyashita K
National Institute For Agro-environmental Sciences
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Miyashita Kiyotaka
National Inst. For Agro-environmental Sciences
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Fujii Takeshi
National Inst. For Agro-environmental Sciences
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