Molecular Characterization of a Novel Family-46 Chitosanase from Pseudomonas sp. A-01
スポンサーリンク
概要
- 論文の詳細を見る
Pseudomonas sp. A-01, isolated as a strain with chitosan-degrading activity, produced a 28 kDa chitosanase. Following purification of the chitosanase (Cto1) and determination of its N-terminal amino acid sequence, the corresponding gene (cto1) was cloned by a reverse-genetic technique. The gene encoded a protein, composed of 266 amino acids, including a putative signal sequence (1-28), that showed an amino acid sequence similar to known family-46 chitosanases. Cto1 was successfully overproduced and was secreted by a Brevibacillus choshinensis transformant carrying the cto1 gene on expression plasmid vector pNCMO2. The purified recombinant Cto1 protein was stable at pH 5–8 and showed the best chitosan-hydrolyzing activity at pH 5. Replacement of two acidic amino acid residues, Glu23 and Asp41, which correspond to previously identified active centers in Streptomyces sp. N174 chitosanase, with Gln and Asn respectively caused a defect in the hydrolyzing activity of the enzyme.
- 社団法人 日本農芸化学会の論文
- 2008-08-23
著者
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Saito Akiko
Biotechnology Center Toyama Prefecture
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Saito Akiko
Department Of Applied Biological Chemistry The University Of Tokyo
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Ando A
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Nagata Y
Department Of Biotechnology University Of Tokyo
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Nagata Yuji
Department Of Applied And Environmental Chemistry Kitami Institute Of Technology
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Ando Akikazu
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Ando Akikazu
Graduate School Of Science And Technology Chiba University
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Nagata Yoshiho
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Nagata Yoshiho
Dep. Of Bioresources Chemistry Fac. Of Horticulture Chiba Univ.
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Saito Akihiro
Department Of Applied Biology And Chemistry Tokyo University Of Agriculture
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Shida Osamu
R&d Department Higeta Shoyu Co. Ltd.
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ARAI Sayaka
Graduate School of Science and Technology, Chiba University
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USUDA Sakiko
Graduate School of Science and Technology, Chiba University
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FURUNO Maiko
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
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KANEKO Naomi
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
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SHIDA Osamu
R&D Department, Higeta Shoyu, Co., Ltd.
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Arai Sayaka
Graduate School Of Science And Technology Chiba University
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Ando Akikazu
Dep. Of Nanobiology Graduate School Of Advanced Integration Sci.
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Furuno Maiko
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Usuda Sakiko
Graduate School Of Science And Technology Chiba University
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Saito Akihiro
Department Of Applied Biology And Chemistry Faculty Of Applied Bio-science Tokyo University Of Agric
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Kaneko Naomi
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Nagata Y
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Ando Akikazu
Graduate School of Advanced Integration Science, Chiba University
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