Domain Construction of Cherry-tomato Lectin : Relation to Newly Found 42-kDa Protein
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概要
- 論文の詳細を見る
In the early stage of ripening of cherry-tomato fruits (Lycopersicon esculentum var. cherry), the lectin activity increased logarithmically and reached a plateau at day 10 after flowering. During purification of lectin from ripe and unripe fruits, a 42-kDa protein was found abundantly in unripe fruits. The protein cross-reacted with anti-cherry-tomato-lectin serum, retained chitin-binding ability, but showed no lectin activity. Comparative studies between the structure of the lectin and the 42-kDa protein were done. N-Terminal amino acid sequences of the lectin, peptides derived from the S-pyridylethylated lectin, and fragments generated by limited proteolysis of the native lectin showed that the lectin was comprised of three domains, Hyp-rich, Cysrich, and Gln-rich, and the alignment of them was as this order from the N-terminus. Studies on the 42-kDa protein showed that it contained two of the three domains, Cys-rich and Gln-rich, but the amino acid sequence analysis showed that the protein should be a product of another gene.
- 社団法人日本農芸化学会の論文
- 2001-01-23
著者
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Ando A
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Nagata Y
Department Of Biotechnology University Of Tokyo
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Nagata Yuji
Department Of Applied And Environmental Chemistry Kitami Institute Of Technology
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Oguri S
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Ando Akikazu
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Ando Akikazu
Department Of Agricultural Chemistry Chiba University
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Nagata Yoshiho
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Oguri Suguru
Department Of Bioproduction Faculty Of Bioindustry Tokyo University Of Agriculture
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NAITO Yoshikazu
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
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MINAMIHARA Tomoyuki
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
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MARUTANI Tazunu
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
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Naito Yoshikazu
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Minamihara Tomoyuki
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Marutani Tazunu
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Nagata Y
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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