Molecular Structure and Properties of Lectin from Tomato Fruit
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概要
- 論文の詳細を見る
A cDNA encoding tomato fruit lectin was cloned from an unripe cherry-tomato fruit cDNA library. The isolated lectin cDNA contained an open reading frame encoding 365 amino acids, including peptides that were sequenced. The deduced sequence consisted of three distinct domains: (i) an N-terminal short extensin-like domain; (ii) a Cys-rich carbohydrate binding domain composed of four almost identical chitin-binding domains; (iii) an internal extensin-like domain of 101 residues containing 15 SerPro4 motifs inserted between the first and second chitin-binding domains. The molecular weight of the lectin was 65,633 and that of the deglycosylated lectin was 32,948, as determined by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). This correlated with the estimated molecular weight of the deduced sequence. Recombinant tomato lectin expressed in Pichia pastoris possessed chitin-binding but not hemagglutinating activity. These findings confirmed that the cDNA encoded tomato lectin.
- 社団法人 日本農芸化学会の論文
- 2008-10-23
著者
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Oguri Suguru
Department of Bioproduction, Faculty of Bioindustry, Tokyo University of Agriculture
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MOMONOKI Yoshie
Department of Bioproduction, Faculty of Bioindustry, Tokyo University of Agriculture
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Nagata Yoshiho
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Nakashita Hideo
Plant Acquired Immunity Research Unit Discovery Research Institute Riken
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Nakashita Hideo
Plant Acquired Immunity Research Unit Advanced Science Institute Riken
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Momonoki Yoshie
Department Of Bioproduction Faculty Of Bioindustry Tokyo University Of Agriculture
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Amano Koh
Research Center For Medical Glycoscience National Institute Of Advanced Industrial Science And Techn
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Oguri Suguru
Department Of Bioproduction Faculty Of Bioindustry Tokyo University Of Agriculture
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