Molecular Properties of Mycelial Aggregate-Specific Lectin of Pleurotus cornucopiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
By cloning and sequencing cDNA, the primary structure of a mycelial aggregate-specific lectin of Pleurotus cornucopiae was determined. The amino acid sequence was novel and elucidated unique properties of this lectin : It was composed of 373 amino acids, 33 of which constitute a signal sequence. The sequence of the mature lectin consisted of two homologous regions having five glycosylation recognition signals and six cysteine residues. However, the distribution of these elements in the two regions was biased. Expression of cDNA in Escherichia coli and Pichia pastoris revealed the requirement of glycosylation to produce the functional lectin. Gel filtration followed by gel electrophoretic analyses of the purified lectin showed that the active component moved faster than the bulk of the protein, suggesting that the most active lectin formed an oligomer of subunits through disulfide bonds. From these observations, a model for the structure of the active form of this lectin is proposed. Southern hybridization using the cDNA as a probe revealed the presence of several genes. The lectin gene was composed of five exons and five introns.
- 社団法人日本生物工学会の論文
- 2004-10-25
著者
-
ANDO Akikazu
Department of Biotechnology, Graduate School of Science and Technology, Chiba University
-
NAGATA Yoshiho
Department of Biotechnology, Graduate School of Science and Technology, Chiba University
-
Oguri Suguru
Department of Bioproduction, Faculty of Bioindustry, Tokyo University of Agriculture
-
Yamaguchi Hiroko
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
-
SUMISA FUTOSHI
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
-
ICHIJO NAOYA
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
-
NAKATSUMI HARUKO
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
-
IIJIMA NAOTO
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
-
UEHARA KOICHI
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
-
Ichijo Naoya
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
-
Uehara Koichi
Department Of Applied Chemistry School Of Science And Engineering Waseda University
-
Sumisa Futoshi
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
-
Nakatsumi Haruko
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
-
Ando A
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
-
Nagata Y
Department Of Biotechnology University Of Tokyo
-
Nagata Yuji
Department Of Applied And Environmental Chemistry Kitami Institute Of Technology
-
Iijima Naoto
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
-
Oguri S
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
-
Ando Akikazu
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
-
Ando Akikazu
Department Of Agricultural Chemistry Chiba University
-
Nagata Yoshiho
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
-
Oguri Suguru
Department Of Bioproduction Faculty Of Bioindustry Tokyo University Of Agriculture
-
Nagata Y
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
関連論文
- An Enzyme-Linked Immunosorbent Assay (ELISA) to Determine the Specificity of the Sugar-Binding Protein NgcE, a Component of the ABC Transporter for N-Acetylglucosamine in Streptomyces olivaceoviridis
- First Clinical Isolates of Nocardia carnea, Nocardia elegans, Nocardia paucivorans, Nocardia puris and Nocardia takedensis in Japan
- Transvalencin A, a Thiazolidine Zinc Complex Antibiotic Produced by a Clinical Isolate of Nocardia transvalensis : II. Structure Elucidation
- Transvalencin A, a Thiazolidine Zinc Complex Antibiotic Produced by a Clinical Isolate of Nocardia transvalensis : I. Taxonomy, Fermentation, Isolation and Biological Activities
- Isolation and Structure Elucidation of Two New Antibacterial Compounds Produced by Chrysosporium queenslandicum
- Asterobactin, a New Siderophore Group Antibiotic from Nocardia asteoides
- Queenslandon, a New Antifungal Compound Produced by Chrysosporium queenslandicum : Production, Isolation and Structure Elucidation
- Structures of ADP-Ribosylated Rifampicin and Its Metabolite : Intermediates of Rifampicin-ribosylation by Mycobacterium smegmatis DSM43756
- Identification of Insertion Sequence from a γ-Hexachlorocyclohexane Degrading Bacterium, Sphingomonas paucimobilis UT26
- Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis
- Isolation and Characterization of Tn5-Induced Mutants of Pseudomonas paucimobilis UT26 Defective in γ-Hexachlorocyclohexane Dehydrochlorinase (LinA)
- Dehydrochlorination of γ-Hexachlorocyclohexane (γ-BHC) by γ-BHC-Assimilating Pseudomonas paucimobilis(Microbiology & Fermentation Industry)
- Pseudomonas fluorescens KKL101, a Benzoic Acid Degrader in a Mixed Culture That Degrades Biphenyl and Polychlorinated Biphenyls
- Molecular Structure and Properties of Lectin from Tomato Fruit
- Molecular Properties of Mycelial Aggregate-Specific Lectin of Pleurotus cornucopiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Two Genes Encoding Fruit Body Lectins of Pleurotus cornucopiae : Sequence Similarity with the Lectin of a Nematode-Trapping Fungus(Biochemistry & Molecular Biology)
- Microbial Growth on a Medium Containing Methanol and Bicarbonate under Anaerobic Conditions in the Light
- Antitumor Activity of Brasilicardin A, a Novel Terpenoid Antibiotic from Nocardia brasiliensis
- Brasilicardin A, a New Terpenoid Antibiotic from Pathogenic Nocardia brasilienesis: Fermentation, Isolation, and Biological Activity
- Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
- Heterotactic Polymerization of Methacrylates Having C-3 Ester Group
- Isolation and Identification of a Novel Strain of the Genus Ochrobactrum with Phenol-Degrading Activity(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Isolation and Characterization of Phenol-catabolizing Bacteria from a Coking Plant(Microbiology & Fermentation Technology)
- Kinetic Rate Constant for Electron Transfer between Ferrous Ions and Novel Rusticyanin Isoform in Acidithiobacillus ferrooxidans
- Respiratory Isozyme, Two Types of Rusticyanin of Acidithiobacillus ferrooxidans(Microbiology & Fermentation Technology)
- Growth Inhibition by Metabolites of γ-Hexachlorocyclohexane in Sphingobium japonicum UT26
- Production of Conjugated Fatty Acids by Lactic Acid Bacteria
- A Lectin from an Ascomycete Mushroom, Melastiza chateri : No Synthesis of the Lectin in Mycelial Isolate
- Enhancement of ascospore germination from Aleuria aurantia after cold storage
- Synthesis of a Lectin in Both Mycelia and Fruit Bodies of the Ascomycete Mushroom Aleuria aurantia
- The lin Genes for γ-Hexachlorocyclohexane Degradation in Sphingomonas sp. MM-1 Proved to Be Dispersed across Multiple Plasmids
- Bacillus circulans MH-K1 Chitosanase : Amino Acid Residues Responsible for Substrate Binding
- Involvement of Tyrosines at Fucose-binding Sites of Aleuria aurantia Lectin : Non-equal Response to Site-directed Mutagenesis among Five Sites
- Structural Analysis of Conjugated Linoleic Acid Produced by Lactobacillus plantarum, and Factors Affecting Isomer Production(Microbiology & Fermentation Technology)
- Ricinoleic Acid and Castor Oil as Substrates for Conjugated Linoleic Acid Production by Washed Cells of Lactobacillus plantarum(Microbiology & Fermentation Technology)
- Increased Antioxidative Activity of Ovalbumin by Heat Treating in an Emulsion of Linoleic Acid
- Purification and Characterization of an Extracellular Chitosanase Produced by Amycolatopsis sp. CsO-2
- Purification and Properties of Amylases Extracellularly Produced by an Imperfect Fungus, Fusidium sp. BX-1 in a Glycerol Medium
- Purification and Properties of an Alcohol Dehydrogenase Isozyme from a Methanol-using Yeast, Candida sp. N-16
- Enzymatic Synthesis of Useful Alkyl β-Glucosides(Biological Chemistry)
- The Possibility of Enzymatic Synthesis of a Variety of β-Xylosides Using the Transfer Reaction of Aspergillus niger β-Xylosidase(Biological Chemistry)
- Purification and Characterization of Isocitrate Lyase from Rhodopseudomonas sp. No.7(Microbiology & Fermentation Industry)
- Growth and Levels of Isocitrate Lyase in Rhodopseudomonas sp. No. 7 Incubated in a Medium Containing Ethanol and Dicarboxylic Acid(Microbiology & Fermentation Industry)
- Isozyme Patterns of Alcohol Dehydrogenases in a Methanol-utilizing Yeast, Candida sp. N-16(Microbiology & Fermentation Industry)
- Intraspecific Hybridization of a Methanol-utilizing Yeast, Candida sp. N-16, through Protoplast Fusion(Microbilolgy & Fermentation Industry)
- Isolation and Characterization of Tn5-Induced Mutants of Sphingomonas paucimobilis Defective in 2,5-Dichlorohydroquinone Degradation
- Immunomagnetic Isolation of Cryptococcus neofarmans by Beads Coated with Anti-Cryptococcus Serum
- Unique and Convenient Use of Raney Nickel for the Reduction of Aryl Bromides, Benzyl Alcohols, Benzyl Ethers, and Benzylamines in an Acidic Medium
- Electrooxidative Conversion of Aldehyde and Ketone Phenylhydrazones into the Methoxy(phenylazo) alkanes
- Isolation, Crystallization, and Characterization of a 16.5-kDa Protein from Fruit Bodies of a Lectin-deficient Strain of Pleurotus cornucopiae
- Purification and Properties of Lectins from a Mushroom, Pleurotus cornucopiae
- Characterization, Occurrence, and Molecular Cloning of a Lectin from Grifola frondosa : Jacalin-Related Lectin of Fungal Origin
- Properties of Mycelial Aggregate-specific Lectin of Pleurotus cornucopiae Produced in Pichia pastoris
- Production of Fruiting-Body Lectins of Pleurotus cornucopiae in Methylotrophic Yeast Pichia pastoris
- Enzymatic Synthesis of 4-Hydroxyphenyl β-D-Oligoxylosides and Their Notable Tyrosinase Inhibitory Activity
- Enantioselecitve Elimination of Pd-H from η^3-Allylpalladium-Tol BINAP Complexes. Evidence of Syn Elimination Pathway
- Utilization of Alcohols by Rhodopseudomonas sp. No. 7 Isolated from n-Propanol-Enrichment Cultures
- Production of Functional Lectin in Pichia pastoris Directed by Cloned cDNA from Aleuria aurantia(Biochemistry & Molecular Biology)
- Molecular Structure and Properties of Lectin from Tomato Fruit
- The lin Genes for γ-Hexachlorocyclohexane Degradation in Sphingomonas sp. MM-1 Proved to Be Dispersed across Multiple Plasmids
- Molecular Characterization of a Novel Family-46 Chitosanase from Pseudomonas sp. A-01
- Complete Amino Acid Sequence of a Lectin-related 16.5-kDa Protein Isolated from Fruit Bodies of a Lectin-deficient Strain of Pleurotus cornucopiae
- Domain Construction of Cherry-tomato Lectin : Relation to Newly Found 42-kDa Protein
- Nuclear-Encoded Plastid Sigma Factor SIG6 Exclusively Contributes to Chloroplast Differentiation in Plastid Differentiation of Arabidopsis thaliana
- Effect of Methanol Concentration on Levels of Methanol Dissimilating Enzymes in Candida sp. N-16
- Purification, Characterization, and Molecular Cloning of Lectin from Winter Buds of Lysichiton camtschatcensis (L.) Schott
- Aleuria aurantia Lectin Exhibits Antifungal Activity against Mucor racemosus
- Vibration spectra and rotational isomerism of chain molecules. VII. 1-Chloro-, 1-bromo-, and 1-iodopropanes, and 1-chloro-, 1-bromo-, and 1-iodobutanes.
- Anodic oxidation of N,N-dimethyl-m-toluidine at a platinum electrode in an acetonitrile solution.
- The crystal and molecular structure of silver (I) N,N-diethyldithiocarbamate.
- The crystal and molecular structure of the copper(I) chloride complex containing p-benzoquinone as a ligand.
- Copper(I) chloride complex containing p-benzoquinone as a ligand.
- Employment of the Human Estrogen Receptor .BETA. Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters.