Isolation and Characterization of Tn5-Induced Mutants of Sphingomonas paucimobilis Defective in 2,5-Dichlorohydroquinone Degradation
スポンサーリンク
概要
- 論文の詳細を見る
Sphingomonas paucimobilis UT26 uses γ-hexachlorocyclohexane (γ-HCH) as a sole source of carbon and energy. In UT26, γ-HCH is converted to 2,5-dichlorohydroquinone (2,5-DCHQ). To clone a gene that is involved in the degradation of 2,5-DCHQ, a Tn5 mutation was introduced into UT26, and three mutants (UT102, UT103, and UT116) defective in 2,5-DCHQ degradation were visually selected by spraying γ-HCH solution on the colonies. 2,5-DCHQ and hydroquinone (HQ) degradation activities of these three mutants and UT26 were analyzed using resting cells. The three mutants had different phenotypes. It was also demonstrated that 2,5-DCHQ and HQ degradation activities were inducibly expressed in UT26 in the presence of 2,5-DCHQ.
- 社団法人日本農芸化学会の論文
- 1996-04-23
著者
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Takagi M
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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MIYAUCHI Keisuke
Department of Civil and Environmental Engineering, Tohoku Gakuin University
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NAGATA Yuji
Department of Biotechnology, University of Tokyo
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Takahashi Maki
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Miyauchi Keisuke
Department Of Bioengineering Nagaoka University Of Technology
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Takagi M
Department Of Biotechnology University Of Tokyo
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Nagata Y
Department Of Biotechnology University Of Tokyo
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Nagata Yuji
Department Of Applied And Environmental Chemistry Kitami Institute Of Technology
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SUH Seug-Kyo
Department of Environmental Management, Shinil Christian College
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FUTAMURA Akiko
Department of Biotechnology, The University of Tokyo
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Suh Seug-kyo
Department Of Environmental Management Shinil Christian College
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Futamura Akiko
Department Of Biotechnology The University Of Tokyo
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Takahi Masamichi
Department Of Biotechnology The University Of Tokyo
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Nagata Y
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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