Cloning and Characterization of a Chitinase-encoding Gene (chiA) from Aspergillus nidulans, Disruption of Which Decreases Germination Frequency and Hyphal Growth
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概要
- 論文の詳細を見る
We cloned a chitinase-encoding gene from Aspergillus nidulans by polymerase chain reaction using degenerated oligonucleotide primers designed from the conserved amino acid sequences among chitinases from yeasts and Rhizopus spp. The cloned gene, named chiA, encoded a polypeptide consisting of 660 amino acids. Disruption of chiA had no effect on hyphal or conidiophore morphology, but germination frequency and hyphal growth rate decreased substantially. Expression of chiA was investigated using Escherichia coli β-galactosidase as a reporter enzyme. The β-galactosidase activity was present during hyphal growth and increased twice as the conidiophores developed. In situ staining of β-galactosidase activity found high expression in metulae, phialides, and conidia during conidiophore development, indicating that the expression of chiA is developmentally regulated. This is the first report to isolate a chitinase gene from A. nidulans and investigate its functions using the gene disruption technique and gene fusion methods in filamentous fungi.
- 社団法人日本農芸化学会の論文
- 1998-01-23
著者
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Ohta Atsutane
Health & Bioscience Laboratories Meiji Seika Kaisha Ltd.
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Takagi M
Department Of Biotechnology The University Of Tokyo
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Ohta Akinori
Department of Agricultural Chemistry, The University of Tokyo
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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Takaya N
Univ. Tsukuba Ibaraki Jpn
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Takaya Naoki
Department Of Biotechnology The University Of Tokyo:(present Address)institute Of Applied Biochemist
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Ohta A
Dep. Of Biotechnology The Univ. Of Tokyo
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Ohta A
Univ. Tokyo Tokyo
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Ohta Akinori
Department Of Agricultural Chemistry The University Of Tokyo
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Yamazaki Daisuke
Department Of Electrical And Communication Engineering Graduate School Of Engineering Tohoku Univers
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HORIUCHI HIROYUKI
Department of Surgery, Kurume University School of Medicine
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Ogura Atsushi
Department Of Biotechnology The University Of Tokyo
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Takahashi Maki
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Horiuchi Hiroyuki
Department Of Biotechnology University Of Tokyo
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Horiuchi Hiroyuki
Earth Science Laboratory Faculty Of Education Hirosaki University
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Horiuchi Hiroyuki
Laboratory Of Cellular Genetics Department Of Biotechnology Division Of Agriculture And Agricultural
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Horiuchi Hiroyuki
Department Of Agricultural Chemistry The University Of Tokyo
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Yamazaki Daisuke
Department Of Biotechnology The University Of Tokyo
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Yamazaki Daisuke
Department Of Applied Chemistry Faculty Of Engineering Gunma University
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Takagi M
Department Of Biotechnology University Of Tokyo
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Ogura A
Meiji University
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Takahi Masamichi
Department Of Biotechnology The University Of Tokyo
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