The Effect of Fructooligosaccharides was Analyzed by cDNA Expression Arrays
スポンサーリンク
概要
- 論文の詳細を見る
We have already reported that indigestible fructooligosaccharides (FOS) increased calcium absorption in rat large intestines and that calbindin-D9k (CaBP), which is an intestine-specific calcium-binding protein, is involved in that increasing effect. In this study, not only the CaBP gene, every gene that changed expression profiles as the result of FOS feeding was identified by cDNA expression arrays. Sprague-Dawley male rats were fed an experimental diet containing 10% FOS for 10 d. To compare gene expression with rats fed a control diet, total mRNA was extracted from the colorectum and analyzed using a Rat cDNA Expression Arrays filter. This arrays filter contains probes of 588 genes, and 195 of them showed detectable changes in their expression by FOS feeding. There were six genes that increased their expression more than twice that of the control. Among them, genes related to the induction of cell growth such as Map kinase 1 and Max were included. Expressions that decreased to less than half were observed in 20 genes, such as somatostatin and prohibitin, which prohibit cell growth. These results are consistent with the other observation that FOS increases cell growth in the colorectum. This approach has revealed that cDNA array technology is an effective tool for nutritional sciences that involve the regulation of a large number of genes, especially for molecular mechanisms of regulation, by nutritional constituents.
- 日本ビタミン学会の論文
著者
-
Fukushima Akiko
Department of Neuroscience and Neurology, Osaka City University Medical School
-
Ohta Atsutane
香川栄養大学
-
Ohta Atsutane
Department Of Applied Nutrition Josai International University
-
Ohta Atsutane
Health & Bioscience Laboratories Meiji Seika Kaisha Ltd.
-
Sakai Kentaro
岡山県立大学 保健福祉学部栄養学科
-
SAKUMA Keiko
Department of Molecular Nutrition, Kagawa Nutrition University
-
SAKAI Kensuke
Health & Bioscience Laboratories, Meiji Seika Kaisha, Ltd.
-
Fukushima Akiko
Department Of Molecular Nutrition Kagawa Nutrition University
-
Fukushima Akiko
Department Of Director Nursing Fukuda Hospital
-
Fukushima Akiko
National Institute Of Advanced Industrial Science And Technology (aist) Spintronics Research Center
-
Sakai Kentaro
Dep. Of Nutrition And Health Promotion Fac. Of Human Life Sci. Hiroshima Jogakuin Univ.
-
Sakai Kensuke
Department Of Nutrition And Health Promotion Faculty Of Human Life Science Hiroshima Jogakuin Univer
-
Sakuma Keiko
Department Of Internal Medicine Tokai University School Of Medicine
-
FUKUSHIMA Akiko
Department of Biochemistry, Saitama Medical School, Moroyama, Iruma-gun
関連論文
- ＜Symposium IV＞Oligomeric Aβ is the sole culprit molecule to cause Alzheimer's disease?
- Cell Cycle Analysis of Chinese Hamster Ovary Cells Stimulated by Phosphatidic Acid in Serum-Free Culture(CELL AND TISSUE ENGINEERING)
- Comparative Study on Delivery of Phosphatidic Acid to Serum-Free Culture of Chinese Hamster Ovary Cells(ANIMAL BIOTECHNOLOGY)
- Use of Nonionic Surfactants for Effective Supply of Phosphatidic Acid in Serum-Free Culture of Chinese Hamster Ovary Cells
- Production of Recombinant Protein by Baculovirus-Infected Insect Cells in Immobilized Culture Using Porous Biomass Support Particles
- Effects of Phospholipids on Growth of Chinese Hamster Ovary Cells in Serum-Free Media
- Evidence That the Expression of the Gene for NADPH-Cytochrome P-450 Reductase is n-Alkane-inducible in Candida maltosa
- Evolutionary Position of n-Alkane-assimilating Yeast Candida maltosa Shown by Nucleotide Sequence of Small-subunit Ribosomal RNA Gene
- Phosphatidylserine Synthesis Required for the Maximal Tryptophan Transport Activity in Saccharomyces cerevisiae
- Isolation and Characterization of ECT1 Gene Gncoding CTP: Phosphoethanolamine Cytidylyltransferase of Saccharomyces cerevisiae^1
- Expression of Calbindin-D9k, VDR and Cdx-2 Messenger RNA in the Process by Which Fructooligosaccharides Increase Calcium Absorption in Rats
- The Effect of Fructooligosaccharides was Analyzed by cDNA Expression Arrays
- Electronic Structure of Ce_Nd_xO_ Probed by Soft-X-Ray Spectroscopy
- Band Structure of Sr_Ba_Nb_2O_6 Thin Film Probed by Soft-X-Ray Emission Spectroscopy
- Electronic Structure of Delafossite-Type Oxide PtCoO_2 by Resonant-Photoemission Spectroscopy
- Band Structure of TiO_2-Doped Yttria-Stabilized Zirconia Probed by Soft-X-Ray Spectroscopy
- Cloning and Characterization of a Chitinase-encoding Gene (chiA) from Aspergillus nidulans, Disruption of Which Decreases Germination Frequency and Hyphal Growth
- Cloning and Characterization of the Rhizopus niveus leul Gene and Its Use for Homologous Transformation
- Isolation and Characterization of Two Chitin Synthase Genes from Aspergillus nidulans
- Effect of Citrus Fruit (Sudachi) Juice on Absorption of Calcium from Whole Small Fish in Healthy Young Men
- Japanese Citrus Fruit (Sudachi) Juice Is Associated with Increased Bioavailability of Calcium from Whole Small Fish and Suppressed Bone Resorption in Rats
- IgE Responses in Mice Fed Moderate Protein Deficient and High Protein Diets
- Low-Protein Diet Induces Oral Tolerance to Ovalbumin in Mice
- Effects of pH Variation and NaCl on In Vitro Digestibility of Cow's Milk Proteins in Commercially Available Infant Formulas
- Construction and Some Characterization of a Yeast Artificial Chromosome Library from DNA of a Tomato Line Having Four Disease Resistance Traits
- Identification of cDNA Clones Coding for the Style Specific S_-Glycoprotein Gene Associated with Gametophytic Self-incompatibility in Tomato (Lycopersicon peruvianum)
- Partial Sequence of Acid Phosphatase-1^1 Gene (Aps-1^1) Linked to Nematode Resistance Gene (Mi) of Tomato
- Pseudohyphal Growth in a Dimorphic Yeast, Candida maltosa, after Disruption of the C-GCN4 Gene, a Homolog of Saccharomyces cerevisiae GCN4(Biochemistry & Molecular Biology)
- Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
- Evidence That the Aspergillus nidulans Class I and Class II Chitin Synthase Genes, chsC and chsA, Share Critical Roles in Hyphal Wall Integrity and Conidiophore Development^1
- Cloning and Characterization of EPD2,a Gene Required for Efficient Pseudohyphal Formation of a Dimorphic Yeast, Candida maltosa
- Isolation of a Chitin Synthase Gene (chsC) of Aspergillus nidulans
- Isolation and Characterization of Two Chitin Synthase Genes of Rhizopus oligosporus
- Low Dose Iron Supplementation with Fructooligosaccharides Improves Iron Status and Aerobic Capacity in Women under Moderate Physical Training
- Effect of Dietary Short-Chain Fructooligosaccharides on the Cecal Microflora in Gastrectomized Rats
- Fluorescent In Situ Hybridization Analysis of Cecal Microflora in Rats Simultaneously Administrated Lactobacillus rhamnosus KY-3 and Cellobiose
- Effect of Lactobacillus rhamnosus KY-3 and Cellobiose as Synbiotics on Lipid Metabolism in Rats
- The Specific Expression Patterns of Lactase, Sucrase and Calbindin-D9k in Weaning Rats Are Regulated at the Transcriptional Level
- Transcriptional Regulation of Rat Calbindin Expression during Development Determined by Bacterially Expressed Protein
- Effects of Lower Limb and Dorsolumbar Massages on Edema in Postpartum Women
- Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors
- The Class V Chitin Synthase Gene csmA Is Crucial for the Growth of the chsA chsC Double Mutant in Aspergillus nidulans
- Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae
- Misoflded Membrane-Bound Cytochrome P450 Activates KAR2 Induction through Two Distinct Mechanisms
- Null Mutation in IRE1 Gene Inhibits Overproduction of Microsomal Cytochrome P450Alk1 (CYP 52A3) and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae
- Regulation of Phospholipid Synthesis in Response to Nutritional Environments in Mitochondria of Saccharomyces cerevisiae
- Effect of amino acid mixtures on nasal allergic responses induced by toluene diisocyanate in mice
- Inflammatory myofibroblastic tumor of the uterus with prominent myxoid change
- Determination of Antihypertensive Peptides from an Izumi Shrimp Hydrolysate
- Transcriptional Regulation of Rat Calbindin Expression during Development Determined by Bacterially Expressed Protein
- IgE Responses in Mice Fed Moderate Protein Deficient and High Protein Diets
- Short-Chain Fatty Acids Increase the Level of Calbindin-D9k Messenger RNA in Caco-2 Cells