Production of Recombinant Protein by Baculovirus-Infected Insect Cells in Immobilized Culture Using Porous Biomass Support Particles

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概要

• 論文の詳細を見る

• Immobilization of insect cells using porous biomass support particles (BSPs) and production of a recombinant protein by the immobilized cells after infection with a baculovirus were investigated in a shake-flask culture. Sf9 cells were passively immobilized in reticulated polyvinyl formal (PVF) resin BSPs (2×2×2mm cubes) with matrices of 60 μm mean pore diameter in situ in shake-flasks. The cell density in the BSPs was over 5×10^7 cells/cm^3-BSP in cultures with regular replacement of the culture medium, as estimated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. After infection with a recombinant baculovirus carrying the β-galactosidase gene, immobilized cells within the BSPs showed a high specific productivity, comparable to the maximum productivity in shake-flask cultures of non-immobilized cells, as long as nutrients in the medium were not depleted. Even when immobilized cells at a high density of 5×10^7 cells/cm^3-BSP were infected with the baculovirus, efficient β-galactosidase production with a high specific productivity was possible by replacing the medium at appropriate intervals to avoid nutrient depletion.

• 社団法人日本生物工学会の論文
• 2000-01-25

著者

• 山地 秀樹

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• 山地 秀樹

  神戸大・工

• Yamaji Hideki

  Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University

• IZUMOTO EIJI

  Production Technology RD Center, Kaneka Corporation

• SAKAI KENTARO

  Division of Molecular Science, Graduate School of Science and Technology, Kobe University

• FUKUDA HIDEKI

  Division of Molecular Science, Graduate School of Science and Technology, Kobe University

• TAGAI SHIN-ICHI

  Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University

• IZUMOTO EIJI

  Process Technology & Research Laboratories, Kaneka Corporation

• Izumoto E

  Kaneka Corp. Hyogo Jpn

• Izumoto Eiji

  Production Technology Rd Center Kaneka Corporation

• Sakai Kentaro

  岡山県立大学 保健福祉学部栄養学科

• Sakai Kentaro

  Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K

• Fukuda Hideki

  Organization Of Advanced Science And Technology Kobe University

• Fukuda H

  Kobe Univ. Kobe

• Fukuda Hideki

  Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K

• Yamaji H

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Fukuda H

  Kobe Univ. Kobe Jpn

• Fukuda Hideki

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University

• Sakai K

  Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K

• Tagai Shin-ichi

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Yamaji Hideki

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Fukuda Hideo

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• Sakai Kentaro

  Dep. Of Nutrition And Health Promotion Fac. Of Human Life Sci. Hiroshima Jogakuin Univ.

• Sakai Kensuke

  Department Of Nutrition And Health Promotion Faculty Of Human Life Science Hiroshima Jogakuin Univer

• Fukuda Hideki

  Division Of Bioscience The Graduate School Of Science And Technology Kobe University

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