A Simple Method for Measuring the Complement Activities of Both Classical and Alternative Pathways by Using Rabbit γ-Globulin-Coupled Liposomes

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概要

• 論文の詳細を見る

• Rabbit γ-globulin (RGG) was covalently coupled to liposomes in which carboxyfluorescein was encapsulated as a marker. Lysis of the liposomes was observed under incubation with guinea pig complement. However, nolysis was observed with heat-inactivated complement. These results suggest that lysis of the liposomes is caused by the activation of complement with coupled RGG in particular, through the alternative parhyway of complement (APC). By addition of anti-rabbit IgG antibodies to the RGG-coupled liposomes, lysis of the liposomes was remarkably enhanced. This may be due to the additional activation of the classical pathway of complement (CPC) by antigen-antibody complexes formed on the surface of liposomes. Thus, the activities of both the pathways can be measured easilty in the same system by using RGG-coupled liposomes.

• 社団法人日本生物工学会の論文
• 1989-12-25

著者

• 山地 秀樹

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• KATOH Shigeo

  Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto Un

• Yamaji H

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Fukuda H

  Kobe Univ. Kobe Jpn

• TERASHIMA Masaaki

  Department of Biosphere Sciences, School of Human Sciences, Kobe College

• FUKUDA HIDEKI

  Engineering Research Laboratories, Kanegafuchi Chemical Industry Co., Ltd.,

• Terashima Masaaki

  Department Of Biosphere Sciences Kobe College

• Fukuda Hideo

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• Fukuda Hideki

  Engineering Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.

• Katoh S

  Graduate School Of Science And Technology Kobe University

• Katoh Shigeo

  Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University

• KISHIMURA MASAAKI

  Engineering Research Laboratories, Kanegafuchi Chemical Industry Co., Ltd.,

• YAMAJI HIDEKI

  Engineering Research Laboratories, Kanegafuchi Chemical Industry Co., Ltd.,

• SADA EIZO

  Department of Chemical Engineering, Faculty of Engineering, Kyoto University

• Terashima M

  Kyoto Univ. Kyoto Jpn

• Sada E

  Kyoto Univ. Kyoto

• Sada Eizo

  Department Of Chemical Engineering Faculty Of Engineering Kyoto Univeristy

• Kishimura Masaaki

  Engineering Research Laboratories Kaneka Corp.

• KATOH SHIGEO

  Department of Chemical Engineering, Kyoto University

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