Culture Conditions for Intracellular Lipase Production by Rhizopus chinensis and Its Immobilization within Biomass Support Particles
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概要
- 論文の詳細を見る
Acetone-dried cells of Rhizopus chinensis (with a 1,3-positional specificity lipase) were investigated for the interesterification reaction of olive oil and methyl stearate. First, the culture conditions for intracellular lipase production were examined, and then the activities of dried cells obtained from immobilization in Biomass Support Particles (BSPs) were compared with those of freely suspended cells.It was clear from cultivation of freely suspended cells that intracellular lipase activity for the interesterification reaction was enhanced significantly by the presence of oleic acid, olive oil, and tea oil, but that the presence of glucose reduced the activity.The specific activity of dried cells within BSPs increased 7-fold compared with that obtained from freely, suspended cells.The process presented here, using immobilization within BSPs, can provide cells directly as a catalyst with high activity, where cells become immobilized simply during batch operation, and no special preparation of cells is necessary. Therefore, the reaction system using dried cells immobilized within BSPs is a promising interesterification process for industial applications.
- 社団法人日本生物工学会の論文
- 1988-08-25
著者
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Fukuda H
Kobe Univ. Kobe Jpn
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FUKUDA HIDEKI
Engineering Research Laboratories, Kanegafuchi Chemical Industry Co., Ltd.,
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NAKASHIMA TOSHIMITSU
Engineering Research Laboratories, Kanegafuchi Chemical Industry Co.Ltd.
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Nakaishi Tomoyuki
Department Of Biotechnology Graduate School Of Engineering Osaka University:the Chemo-sero-therapeut
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Fukuda Hideo
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Fukuda Hideki
Engineering Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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MORIKAWA HISASHI
Engineering Research Laboratory, Kanegafuchi Chemical Industry Co., Ltd.,
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Nakashima T
Department Of Biotechnology Graduate School Of Engineering Osaka University:the Chemo-sero-therapeut
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KYOTANI SUSUMU
Engineering Research Laboratories, Kanegafuchi Chemical Industry Co., Ltd.,
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Kyotani Susumu
Engineering Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Morikawa H
National Space Development Agency Of Japan Tokyo Jpn
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Nakashima Toshimitsu
Engineering Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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MORIKAWA HISASHI
Engineering Research Laboratories, Kanegafuchi Chemical Industry Co., Ltd.,
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