Intracellular Lipase Production by Rhizopus chinensis Using Biomass Support Particles in a Circulating Bed Fermentor
スポンサーリンク
概要
- 論文の詳細を見る
To utillize Rhizopus chinensis cells directly as a catalyst for industrial interesterification of oils and fats, the mass production of acetone-dried cells immobilized Biomass Support Particles (BSPs) which show high intracellular lipase activity was examined using a circulating bed fermentor (CBF). First, the effects of aeration rate and number of BSPs were investigated in batch culture. The results revealed that the aeration rate had a signification influence on the formation of intracellular lipase. Therefore, a comparatiely low aeration rate (4 vvm) capable of attaining good circulation of BSPs in the CBF was adequate for the lipase production. The effects of meat extract (main component of medium) feeding on intracellular lipase production was also examined by constant-feed rate fed-batch cultures. The results inducated that the specific feed rate of meat extract q_s controlled in the range of 0.04-0.08 (g-meat extract)(g-cell)^<-1>(h)^<-1> might enhance intracellular lipase activity, whereas the cellular growth in BSPs was independent of the meat extract feed rate. Therefore, the fed-batch culture in which the specific feed rate of meat extract q_s was controlled at 0.05 by varying the meat extract feed rate stepwise using a microcomputer was carried out, and the intracellular lipase activity of immobilized cells could be enhanced 1.3-1.5 times that obtained in batch cultivation. The specific feed rate of meat extract q_s is an effective index for the production of intracellular lipase of Rh. chinensis.
- 公益社団法人日本生物工学会の論文
- 1989-07-25
著者
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NAKASHIMA TOSHIMITSU
Engineering Research Laboratories, Kanegafuchi Chemical Industry Co.Ltd.
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Fukuda Hideki
Engineering Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Nagai Shiro
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Nojima Yasuhiro
Engineering Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Nakashima Toshimitsu
Engineering Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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