Fat Hydrolysis and Esterification by a Lipase from Humicola lanuginosa
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概要
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The hydrolysis and esterification by a thermostable lipase from Humicola lanuginosa No. 3 were investigated. Both reactions occurred readily at temperatures between 45-50°C. Esterification by the enzyme with glycerol was observed to be specific towards fatty acids with carbon numbers of C12-C18. Laurie acid esters with different alcohols such as primary alcohols, terpene alcohols, etc., were also synthesized readily. Esterification by the enzyme was adversely affected by the water content (optimum, ca. 7%), however, the hydrolysis rate increased rapidly with increasing water content (optimum, ca. 60%). The enzyme showed increased activity in organic solvent-aqueous reaction systems. Nevertheless, hydrolysis in complete organic phase reactions was found not to be feasible. Hydrolysis at a higher temperature (50 or 55°C) in a solvent free phase was almost the same as that in organic solvent-aqueous phase reactions. The components of glycerides varied considerably during hydrolysis, whereby esterification resulted in a higher quantity of mono-and diglycerides (about 40%), compared to in the case of hydrolysis, for which the value was about 10-20%.
著者
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Nagai Shiro
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Nishio Naomichi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima Univeisity
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Omar IBRAHIM
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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