Asymmetric Reduction of Ethyl Acetoacetate to Ethyl(R)-3-Hydroxybutyrate Coupled with Nitrate Reduction by Paracoccus denitrificans
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概要
- 論文の詳細を見る
We found that the asymmetric reduction of ethyl acetoacetate(EAA)to ethyl(R)-3-hydroxybutyrate(EHB)by Paracoccus denitrificans could be induced by nitrate addition under anaerobic conditions. However, the addition of electron donors such as glucose, ethanol, and methanol together with nitrate did not stimulate EHB production from EAA. When the reaction was carried out under optimum conditions(cell concentration, 10 g-d.w.l^<-1> ; EAA, 150 mM ; NO_3^-, 100 mM), after 8 h of reaction 49 mM of EHB with an optical purity of 98.9% e.e.was produced. Furthermore, a fed-batch reaction was carried out with an intermittent addition of nitrate and EAA to reduce substrate inhibition. The linear reduction of EAA to EHB proceeded for 40 h after initiation of the reaction, and finally 124 mM of EHB with an optical purity of 88.7% e.e.was produced after 104 h.
- 社団法人日本生物工学会の論文
- 2001-04-25
著者
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Kubota Hiroshi
Department of Chemistry, Faculty of Science, Kyoto University
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Nishio N
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Nishio Naomichi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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NAKASHIMADA YUTAKA
Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University
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KAKIZONO TOSHIHIDE
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Kakizono T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kakizono Toshihide
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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TAKAYOSE AKIHIRO
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Nakashimada Y
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Nakashimada Yutaka
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kubota Hiroshi
Department Of Cardiovascular Medicine Kyushu University Graduate School Of Medical Sciences
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Nishio Naomichi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima Univeisity
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Nakashimada Yutaka
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Kubota Hiroshi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kubota Hiroshi
Department Of Appl Ed Physics Sccond Faculty Of Engineering Tokyo Univ.
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Takayose Akihiro
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima University
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